Modification method for leukocyte filtering membrane

Abstract

The invention relates to a modification method for a leukocyte filtering membrane. The modification method includes the following steps that S1, a hydrophobic resin filtering membrane is put in first modification mixed liquid for first-time impregnation for 0.5-2 h at the temperature of 20-40 DEG C and the pressure of 1-10 MPa, the hydrophobic resin filtering membrane is taken out, washed and then dried for 6-10 h at the temperature of 50-60 DEG C, and a first modified filtering membrane is obtained; S2, the first modified filtering membrane is put in second modification mixed liquid for second-time impregnation for 0.5-2 h at the temperature of 20-40 DEG C, the first modified filtering membrane is taken out, washed and then dried for 6-10 h at the temperature of 50-60 DEG C, and the modified filtering membrane is obtained. The modified filtering membrane obtained through the method has good wettability, stability and compatibility, blood platelets in fresh blood and blood preserved at normal temperature are activated to capture leukocyte, it is achieved that the leukocyte and the blood platelets are filtered out at the same time, and the filtering effect is good.

Description

technical field

[0001] The invention belongs to the technical field of leukocyte filtration, and in particular relates to a method for modifying a leukocyte filtration membrane. Background technique

[0002] Unprocessed whole blood or blood component preparations contain a large number of white blood cells, which will cause many side effects when transfused into the patient's body, so it is of great significance to filter out white blood cells. The leukocyte filtration method uses non-woven fabrics or porous materials with intercommunicating spaces as a filter membrane to filter out leukocytes, and is widely used in the filtration of leukocytes.

[0003] The leukocyte filter membrane is generally made of a non-woven filter membrane made of very fine fiber materials. The ideal filter membrane should have the following characteristics: ultra-fine fiber diameter, small pore size of the filter material, large porosity, and excellent adsorption and wetting properties on the surfa...

Examples

Embodiment 1

[0026] Embodiment 1 The first modified mixed solution

[0027] The first modified mixed solution is obtained by adding polyvinylpyrrolidone and fatty alcohol polyoxyethylene ether into water at a mass ratio of 3:1 and stirring, and the mass fraction is 4%.

Embodiment 2

[0028] Example 2 The first modified mixed solution

[0029] The first modified mixed solution is obtained by adding polyvinylpyrrolidone and fatty alcohol polyoxyethylene ether into water at a mass ratio of 4:1 and stirring, and the mass fraction is 5%.

Embodiment 3

[0032] Example 3 The second modified mixed solution

[0033] The second modified mixed solution is obtained by adding amidated silk fibroin and polyurethane into water at a mass ratio of 0.8:1 and stirring, and the mass fraction is 8%.

[0034] The preparation of amidated modified silk fibroin comprises the following steps: dissolve silk fibroin in water to make a solution with a concentration of 10 mg / mL, add 1 mol / L aqueous urea solution at a volume ratio of 1:5, mix well, and then use Dilute hydrochloric acid solution to adjust the pH value to 4.75, then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride of 2 times the weight of silk fibroin, react at room temperature for 1h, and Add dilute hydrochloric acid solution appropriately during the process to keep the pH of the reaction solution at 4.75. After the reaction, add acetic acid buffer solution with a pH of 4.75 to decompose excess 1-(3-dimethylaminopropyl)-3-ethyl carbon diimine hydrochloride, and then di...

technical field

[0001] The invention belongs to the technical field of leukocyte filtration, and in particular relates to a method for modifying a leukocyte filtration membrane. Background technique

[0002] Unprocessed whole blood or blood component preparations contain a large number of white blood cells, which will cause many side effects when transfused into the patient's body, so it is of great significance to filter out white blood cells. The leukocyte filtration method uses non-woven fabrics or porous materials with intercommunicating spaces as a filter membrane to filter out leukocytes, and is widely used in the filtration of leukocytes.

[0003] The leukocyte filter membrane is generally made of a non-woven filter membrane made of very fine fiber materials. The ideal filter membrane should have the following characteristics: ultra-fine fiber diameter, small pore size of the filter material, large porosity, and excellent adsorption and wetting properties on the surfa...

Examples

Embodiment 1

[0026] Embodiment 1 The first modified mixed solution

[0027] The first modified mixed solution is obtained by adding polyvinylpyrrolidone and fatty alcohol polyoxyethylene ether into water at a mass ratio of 3:1 and stirring, and the mass fraction is 4%.

Embodiment 2

[0028] Example 2 The first modified mixed solution

[0029] The first modified mixed solution is obtained by adding polyvinylpyrrolidone and fatty alcohol polyoxyethylene ether into water at a mass ratio of 4:1 and stirring, and the mass fraction is 5%.

Embodiment 3

[0032] Example 3 The second modified mixed solution

[0033] The second modified mixed solution is obtained by adding amidated silk fibroin and polyurethane into water at a mass ratio of 0.8:1 and stirring, and the mass fraction is 8%.

[0034] The preparation of amidated modified silk fibroin comprises the following steps: dissolve silk fibroin in water to make a solution with a concentration of 10 mg / mL, add 1 mol / L aqueous urea solution at a volume ratio of 1:5, mix well, and then use Dilute hydrochloric acid solution to adjust the pH value to 4.75, then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride of 2 times the weight of silk fibroin, react at room temperature for 1h, and Add dilute hydrochloric acid solution appropriately during the process to keep the pH of the reaction solution at 4.75. After the reaction, add acetic acid buffer solution with a pH of 4.75 to decompose excess 1-(3-dimethylaminopropyl)-3-ethyl carbon diimine hydrochloride, and then di...