A myasthenia gravis detection kit and application using a combination of non-coding lnc-cxcl1 and coding gene cxcl1 as a detection or diagnostic screening marker

A myasthenia gravis, gene-encoding technology, applied in the biological field, can solve problems such as expression and biological functions that have not been reported

Active Publication Date: 2018-02-23
XIANGYA HOSPITAL CENT SOUTH UNIV
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Problems solved by technology

[0004] The gene encoding cxcl1 is CXCR2 + Chemokine for family cells such as neutrophils; non-coding Lnc-CXCL1 is a non-expressed distinct transcript encoding cxcl1 whose expression and biological function have not been reported

Method used

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  • A myasthenia gravis detection kit and application using a combination of non-coding lnc-cxcl1 and coding gene cxcl1 as a detection or diagnostic screening marker
  • A myasthenia gravis detection kit and application using a combination of non-coding lnc-cxcl1 and coding gene cxcl1 as a detection or diagnostic screening marker
  • A myasthenia gravis detection kit and application using a combination of non-coding lnc-cxcl1 and coding gene cxcl1 as a detection or diagnostic screening marker

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Embodiment Construction

[0030] The conventional reagents used in the examples are all commercially available, and the primer pairs described in the examples are as follows:

[0031] A. The specific PCR primer pair for real-time quantification of non-coding lnc-CXCL1 is as follows:

[0032] Forward primer: 5'-AGTCAGTGAGTCTCTCTCTTCCCT-3' (SEQ ID NO.1);

[0033] Reverse primer: 5'-CACCAGTGAGCTTCCTCCTC-3' (SEQ ID NO.2);

[0034] B. The specific PCR primer pair for real-time quantification of the coding gene cxcl1 is as follows:

[0035] Forward primer: 5'-GCGCGCAGCAGGAGCGT-3' (SEQ ID NO.3);

[0036] Reverse primer: 5'-CACCAGTGAGCTTCCTCCTC-3' (SEQ ID NO.4);

[0037] The real-time quantitative specific PCR primer pair of C, GAPDH is as follows:

[0038] Forward primer: 5'-GAGTCAACGGATTTGGTCGT-3' (SEQ ID NO.5);

[0039] Reverse primer: 5'-TTGATTTTGGAGGGATCTCG-3' (SEQ ID NO.6).

[0040] 1. RNA extraction method:

[0041] (1) Take 5ml of peripheral cubital vein blood sample with EDTA anticoagulant bloo...

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Abstract

The invention discloses a myasthenia gravis detection reagent kit with the combination of non-coded lnc-CXCL1 and a coded gene cxcl1 as a detection or diagnosis screening marker and application. By detecting the expression levels of lnc-CXCL1 and the coded gene cxcl1 in peripheral blood specimens of patients suffering from myasthenia gravis in a relative quantification mode, the reagent kit achieves the effects of screening patients suffering from myasthenia gravis, carrying out QMG on clinical symptoms, and judging hormone therapy.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a myasthenia gravis detection kit and its application using the combination of non-coding lnc-CXCL1 and coding gene cxcl1 as detection or diagnostic screening markers. Background technique [0002] Myasthenia gravis (MG) is the most common autoimmune disease among neuromuscular junction-related diseases. Its typical clinical manifestations are fluctuating systemic skeletal muscle fatigue and weakness, including the involvement of respiratory muscles. Its prevalence in the world is about 40-180 / 1 million, and the annual incidence is about 4-12 / 1 million. In recent years, with the improvement of detection technology, new antibodies have been discovered continuously, and the incidence of MG On the rise, especially in late-onset MG. According to the different parts involved, the age of onset and the type of serum antibody, MG can be divided into different subtypes. Although ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6837C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 罗朝辉刘晓芳徐立群罗梦川杨欢肖波
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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