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Culture medium capable of alleviating browning of blueberry tissue cultured seedlings and preparation method thereof

A technology of tissue culture seedlings and medium, applied in the field of culture medium, can solve the problems of tissue culture seedling death, poisoning of explants, unfavorable rapid propagation of blueberry seedlings, etc., to reduce the formation of brown matter, reduce toxicity, and facilitate operation Effect

Inactive Publication Date: 2016-12-07
JILIN PROVINCE PULAN HIGH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of tissue culture and rapid propagation, because the cells on the wound surface of the explants are injured, the intracellular phenolic compounds are exuded, and brown quinones are formed under the action of phenoloxidase, which accumulate and gradually diffuse in the medium. Inhibit the activity of other enzymes, poison the whole explant, and cause the death of tissue culture seedlings, which is not conducive to the rapid reproduction of blueberry seedlings, and is one of the decisive factors that limit the success of blueberry tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The medium for alleviating the browning of blueberry tissue culture seedlings, the basic medium is WPM, and added with 4.5g / L agar, 0.5g / L hydrolyzed milk protein, 1.0g / L polyvinylpyrrolidone, 0.8g / L vitamin C, 0.8g / L glutathione and 0.6g / L citric acid.

[0019] Its preparation method is:

[0020] Add agar, hydrolyzed milk protein, polyvinylpyrrolidone, vitamin C, glutathione and citric acid into the basic medium, mix evenly, subpackage, and sterilize for later use.

[0021] The packaging specification is 25mL / bottle.

Embodiment 2

[0023] The medium for alleviating the browning of blueberry tissue culture seedlings, the basic medium is WPM, and is added with 3g / L agar, 0.2g / L hydrolyzed milk protein, 0.5g / L polyvinylpyrrolidone, 0.5g / L vitamin C , 0.5g / L glutathione and 0.2g / L citric acid.

[0024] Its preparation method is:

[0025] Add agar, hydrolyzed milk protein, polyvinylpyrrolidone, vitamin C, glutathione and citric acid into the basic medium, mix evenly, subpackage, and sterilize for later use.

[0026] The packaging specification is 20mL / bottle.

Embodiment 3

[0028] The medium for alleviating the browning of blueberry tissue culture seedlings, the basic medium is WPM, and is added with 6g / L agar, 0.8g / L hydrolyzed milk protein, 1.5g / L polyvinylpyrrolidone, 1.2g / L vitamin C , 1.2g / L glutathione and 0.8g / L citric acid.

[0029] Its preparation method is:

[0030] Add agar, hydrolyzed milk protein, polyvinylpyrrolidone, vitamin C, glutathione and citric acid into the basic medium, mix evenly, subpackage, and sterilize for later use.

[0031] The packaging specification is 30mL / bottle.

[0032] The blueberry seedlings were inoculated onto the basic medium WPM and the medium prepared in Examples 1-3 respectively, and the browning of the blueberry seedlings and the survival of the blueberry seedlings on different media were observed after 5 weeks, as shown in Table 1 for details.

[0033] Among them: the browning condition is expressed by the browning rate, and the survival condition is expressed by the survival rate.

[0034] The for...

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PUM

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Abstract

The invention relates to a culture medium, and specifically relates to a culture medium capable of alleviating the browning of blueberry tissue cultured seedlings and a preparation method thereof. The basic culture medium of the culture medium is WPM. The culture medium also comprises the following components: agar (3-6 g / L), lactalbumin hydrolysate (0.2-0.8 g / L), polyvinylpyrrolidone (0.5-1.5 g / L), vitamin C (0.5-1.2 g / L), glutathione (0.5-1.2 g / L), and critic acid (0.2-0.8 g / L). The provided culture medium can prominently alleviate the browning of blueberry tissue cultured seedlings, and greatly increases the survival rate of blueberry tissue cultured seedlings. The preparation method is simple, and is easy to carry out and operate.

Description

technical field [0001] The invention relates to a medium, in particular to a medium for alleviating the browning of blueberry tissue culture seedlings and a preparation method thereof. Background technique [0002] Blueberry is a small berry with blue fruit, delicate pulp and tiny seeds. It has the reputation of "King of Berries" and "Queen of Fruits". The average weight of blueberry fruit is 0.5-2.5g, and the maximum weight is 5g. The edible rate is 100%. It is sweet and sour, and has a pleasant aroma. It is a good product for fresh food. Blueberries are not only rich in conventional nutrients, such as protein, vitamins, minerals and trace elements, but also extremely rich in anthocyanins, flavonoids and polysaccharides, which can relieve visual fatigue and promote visual purple in retinal cells. Quality regeneration, prevention of myopia, improvement and enhancement of vision and other effects. [0003] In addition to relieving visual fatigue, blueberries also have many ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 吴林徐振彪屈新宇
Owner JILIN PROVINCE PULAN HIGH TECH CO LTD