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Method for alleviating browning of Chinese rose tissue culture seedlings

A technology of tissue culture seedlings and browning, which is applied in the field of culture medium, can solve the problems of small quantity, difficult promotion, and limited reproduction speed, etc., and achieve the effects of reducing dispersion, improving survival rate, and reducing toxicity

Inactive Publication Date: 2019-05-28
天津创世生态景观建设股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional propagation method of rose is mainly cuttings, but cuttings of some rare varieties are not easy to take root, and some newly cultivated and introduced fine varieties are difficult to promote in the short term due to the extremely small number, and the propagation speed is greatly restricted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The medium for alleviating the browning of Chinese rose tissue culture seedlings, the basic medium is MS, and is added with 5g / L agar, 0.3g / L hydrolyzed milk protein, 0.5g / L polyvinylpyrrolidone, 0.3g / L activated carbon and 0.3g / L citric acid, mix evenly, pack into 30ml / bottle, sterilize for later use.

Embodiment 2

[0016] The medium for alleviating the browning of Chinese rose tissue culture seedlings, the basic medium is MS, and is added with 6g / L agar, 0.4g / L hydrolyzed milk protein, 1.0g / L polyvinylpyrrolidone, 0.4g / L activated carbon and 0.5g / L citric acid, mix evenly and pack into 30ml / bottle, sterilize for later use.

Embodiment 3

[0018] The medium for alleviating the browning of Chinese rose tissue culture seedlings, the basic medium is MS, and is added with 7g / L agar, 0.5g / L hydrolyzed milk protein, 1.5g / L polyvinylpyrrolidone, 0.5g / L activated carbon and and 0.7g / L citric acid, mix evenly and pack into 30ml / bottles, and sterilize for later use.

[0019] The rose seedlings were inoculated on the basic medium MS and the medium prepared in Examples 1-3 respectively, and the browning of the rose seedlings and the survival of the rose seedlings on different media were observed one month later, see Table 1 for details.

[0020] Among them: the browning condition is expressed by the browning rate, and the survival condition is expressed by the survival rate.

[0021] The formula for calculating the browning rate is: browning rate=the number of browned rose seedlings in each culture bottle / the total number of rose seedlings inoculated in each culture bottle;

[0022] The formula for calculating the survival...

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Abstract

The invention relates to a culture medium, in particular to a method for alleviating browning of Chinese rose tissue culture seedlings. The culture medium is an MS basic culture medium, and added with5-7 g / L of agar, 0.3-0.5 g / L of lactoalbumin hydrolysate, 0.5-1.5 g / L of polyvinylpyrrolidone, 0.3-0.5 g / L of activated carbon and 0.3-0.7 g / L of citric acid. The culture medium can significantly alleviate browning of the Chinese rose tissue culture seedlings, and greatly increases the survival rate of the Chinese rose tissue culture seedlings; and a preparation method is simple and practicable and easy to operate.

Description

technical field [0001] The invention relates to a culture medium, in particular to a method for alleviating browning of Chinese rose tissue cultured seedlings. Background technique [0002] Rose is a perennial woody plant belonging to Rosaceae, which blooms many times a year. The flowers of rose are colorful, rich in flower shape, graceful in posture, with strong, light and different fragrance types. Rose has a long flowering period, wide adaptability, and easy cultivation. The traditional propagation method of rose is mainly cuttings, but cuttings of some rare varieties are not easy to take root, and some newly cultivated and introduced fine varieties are difficult to promote in the short term due to their extremely small number, and the propagation speed is greatly restricted. The tissue culture method provides an effective way for the rapid propagation and popularization of rose. Browning is a serious problem in the tissue culture process, which directly affects the ke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 安振于杰赵卫红刘仕源陈海涛于福满
Owner 天津创世生态景观建设股份有限公司