Method for feeding hirudo nipponia adults and sustainably extracting hirudin and special equipment

A Japanese medical leech and hirudin technology, applied in chemical instruments and methods, from leech inhibitors, protease inhibitors, etc., can solve a large number of problems, achieve the effects of increasing single output, significant economic benefits, and saving resources

Inactive Publication Date: 2016-12-07
重庆志微生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of extracting hirudin from Japanese medical leech is to kill the Japanese medical leech directly and extract it directly from the worm body; a large amount of leech adult resources are required

Method used

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  • Method for feeding hirudo nipponia adults and sustainably extracting hirudin and special equipment
  • Method for feeding hirudo nipponia adults and sustainably extracting hirudin and special equipment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Structure and usage of embodiment 1 special equipment

[0023] The special equipment for the sustainable extraction of hirudin from Japanese medical leech adults is composed of an outer cylinder 1, an inner cylinder 2 and an ultraviolet light source 3; wherein, the outer cylinder 1 is a box-shaped or barrel-shaped structure with an upper opening, and the outer cylinder 1 A support frame 1-1 is provided above, and a valve 1-2 for draining water is provided at the bottom of the outer cylinder 1; the inner cylinder 2 is arranged inside the outer cylinder 1, and the inner cylinder 2 is a box-shaped structure or a barrel-shaped structure with an upper opening, The bottom of the inner cylinder 2 is composed of a filter bottom plate 2-1, the filter bottom plate 2-1 is a sieve plate or screen mesh with an aperture of 20-100 mesh, the inner cylinder 2 is connected with a hook 2-2; the outer light source 3 is arranged on the outer cylinder 1 and the inner The top of the opening a...

Embodiment 2

[0032] The influence of embodiment 2 different emetic agents on Japanese medicine hirudin extraction amount

[0033] Weigh 30kg of adult Japanese leeches, and divide them into 3 groups, respectively named group A, group B and group C, 10kg in each group; first fast the fed adult leeches at 22-25°C for 15 Day, then adopt special equipment under 25-29 ℃ environmental conditions, refer to the method described in Example 1 to extract hirudin. Group A was fed with an emetic with a weight ratio of zinc sulfate, acetic acid and water of 2:5:100; Group A was fed with an emetic with a weight ratio of zinc sulfate, 95% alcohol and water with a weight ratio of 2:5:100; Group C was fed with An emetic with a weight ratio of zinc sulfate and water of 2:100. After each group collects the hirudin extract, add starch and chitosan in a weight ratio of 10:2 to form a stabilizer, and the addition amount is 100 g for each group. Immediately after spray drying, the crude extract of hirudin was ob...

Embodiment 3

[0034] The influence of embodiment 3 different inducing liquid Japanese medicine Hirudin extract amount

[0035] (1) Preparation of induction solution

[0036] Weigh 1000g of fresh eel, cut into pieces and pulverize, put into 5000ml of water, filter through 100 mesh, collect the water, and add 200g of glucose to the water to prepare the eel induction solution;

[0037] Weigh 1000 g of animal liver, cut into pieces and pulverize, put into 5000 ml of water, filter through 100 mesh, collect the water, and add 200 g of glucose to the water to prepare viscera induction solution;

[0038] Weigh 1000g of fresh and alive earthworms, cut into pieces and pulverize, drop into 5000ml water, filter through 100 mesh, collect the water liquid, and add 200g glucose to the water liquid to prepare the earthworm induction liquid;

[0039] (2) Investigation of induction solution

[0040] Weigh 30kg of adult Japanese leeches, and divide them into 3 groups, respectively named group I, group II an...

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PUM

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Abstract

The invention provides a method for feeding hirudo nipponia adults and sustainably extracting hirudin. The method specifically comprises the following steps: 1) satiating hirudo nipponia with induced liquids, and cleaning and cleaning again with saline of which the concentration is 0.6 to 0.8%; 2) adding vomiting liquids according to a weight ratio of the hirudo nipponia to the vomiting liquids of 1: 1; 3) carrying out ultraviolet-irradiation induction on the hirudo nipponia for 2 to 3 times, wherein the induction is carried out for 10 to 15 minutes for each time and at an interval of 10 to 15 minutes for next time; 6) after the irradiation induction is finished, spraying the saline of which the concentration is 0.6 to 0.8% to clean the hirudo nipponia, and collecting cleaning liquids as hirudin extracting solutions. The invention provides the method for sustainably providing the hirudin to save sources of the hirudo nipponia. Furthermore, the extraction vomitive agents provided by the invention can increase the single-yield of the hirudin, so that the method for feeding the hirudo nipponia adults and sustainably extracting the hirudin has significant economic benefits.

Description

technical field [0001] The invention relates to a breeding technology of traditional Chinese medicine animal medicinal materials, and more specifically relates to a method for feeding Japanese medical leech adults and sustainable extraction of hirudin and special equipment. technical background [0002] Japanese medical leech (scientific name: Hirudo nipponia) is an animal of the genus Hirudo in the family Hirudoceae. Distributed in Japan, the Far East of Russia, Mongolia, and the northeastern provinces of mainland China, Inner Mongolia, west to Sichuan, Gansu, south to Guangdong and other places, generally inhabit paddy fields and their connected ditches, ponds and swamps and In particular, retting fields and farmland fields with perennial water accumulation or poor drainage have the largest number. [0003] Hirudin is the most active and most researched active ingredient extracted from Leech or Japanese medical leech and its salivary glands. It is a small molecule compose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/815A01K67/033
CPCC07K14/815A01K67/033
Inventor 李强远杨波杨佐富范桂英刘丽霞
Owner 重庆志微生物技术有限公司
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