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A kind of Escherichia coli movement rate determination method in water and aqueous solution

A technology of Escherichia coli and aqueous solution, applied in the field of instrument analysis, to achieve the effect of no pollution risk and simple operation

Active Publication Date: 2019-06-28
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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Problems solved by technology

Since the size of Escherichia coli is only 0.5-3 microns, the only way to study and measure its movement speed is by microscopic imaging technology.

Method used

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  • A kind of Escherichia coli movement rate determination method in water and aqueous solution
  • A kind of Escherichia coli movement rate determination method in water and aqueous solution

Examples

Experimental program
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Embodiment

[0020] Example Determination of the maximum movement rate of Escherichia coli under the action of gravity in 0.9% NaCl solution at 25°C

[0021] Step 1. Inject 116.56 microliters of 0.9% NaCl solution (5.8 cm in length of the liquid column) into the graduated glass detection tube (6.0 cm in length) with a micro-injector. At this time, the distance between the liquid level at the opening end of the graduated glass detection tube and the receiving electrode is 5.0 cm, that is, the effective motion length L is 5.0 cm.

[0022] Step 2. Insert the scaled glass detection tube into the capacitively coupled non-contact conductivity sensor probe, and adjust the probe bracket so that the detection tube is vertically downward.

[0023] Step 3. Adjust the laboratory temperature to a constant temperature of 25°C.

[0024] Step 4: Centrifuge and wash the Escherichia coli cultured according to conventional methods (4000r / min) for 5 times, and remove the residual culture medium therein. The...

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Abstract

The invention discloses a method for measuring the movement rate of escherichia coli in water and a water solution, and belongs to the technical field of instrument analysis. According to the method, the movement process of escherichia coli in a glass detecting tube is monitored and recorded through a capacity coupling non-contact conductivity sensor. The movement rate R (R=L / T) of escherichia coli is calculated according to the set distance (the effective length L of the scale glass detecting tube) and the consumed time duration T. The method does not need expensive instruments and is convenient and rapid to implement.

Description

technical field [0001] The invention belongs to the technical field of instrument analysis, and in particular relates to a method for measuring the movement rate of Escherichia coli in water and aqueous solution. Background technique [0002] Escherichia coli is a Gram-negative brevibacterium, which is the most dominant and most abundant bacterium in the intestinal tract of humans and many animals, with perinatal flagella. Its movement is mainly driven by the rotation of the flagella (which can be divided into swimming and crawling), so that it can actively respond to external stimuli, such as light, compounds and oxygen. [0003] Like the simplest biological behaviors such as deformation and division, the motility behavior parameters of Escherichia coli are of great significance to many researches in the fields of biology and medicine. By studying its motility, a model system for studying biological behavior can be established, thereby providing basic data for further dete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/10C12R1/19
CPCC12Q1/10
Inventor 曲克明张旭志李秋芬张艳赵俊丁东生黄梦诗杨倩倩
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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