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Stabilizing agent for high-density lipoprotein cholesterol detection reagent

A high-density lipoprotein and detection reagent technology, which is applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc., can solve problems affecting clinical use and poor stability of detection reagents, and achieve good stability, accurate results, and reduced Effect of Blank Absorbance Values

Active Publication Date: 2016-12-07
威海威仕泰医疗科技有限公司
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Problems solved by technology

[0003] At present, high-density lipoprotein cholesterol detection kits mostly use the direct method-selective inhibition method, which is widely used clinically due to its high sensitivity, high accuracy, high precision and applicability, but because the enzyme activity will be affected by pH, The interference of temperature and chemical reagents leads to the problem of poor stability of the detection reagents, which affects clinical use. The storage conditions and period of the high-density lipoprotein cholesterol detection reagents currently on the market are as follows: unopened reagents should be stored in the dark at 2~8°C , the validity period is 12 months; after opening the reagent, store it away from light at 2~8°C, and the validity period is 1 month under the condition of no pollution

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[0013] A stabilizer for high-density lipoprotein cholesterol detection reagent, characterized in that the stabilizer consists of gelatin, MgSO 4 , glycerin, betaine, trehalose, polyethylene glycol, EDTA, polyoxyethylene alkylene tribenzyl phenyl ether, the composition ratio of each component is: gelatin 1 ~ 10ml / L, MgSO 4 0.1~5g / L, glycerin 1~15ml / L, betaine 0.1~1mol / L, trehalose 0.1g / L~20 g / L, polyethylene glycol 0.5~2g / L, EDTA 0.5~2.0g / L , polyoxyethylene alkylene tribenzyl phenyl ether 1 ~ 5g / L, the high-density lipoprotein cholesterol detection reagent is composed of reagent 1 and reagent 2, wherein: R1: PIPES-Na 80mmol / L, cholesteryl ester Enzyme 2KU / L, cholesterol oxidase 2.5KU / L, catalase 120LU / L, 4-aminoantipyrine 240mmol / L, α-cyclodextrin 1g / L, tween-80 3ml / L, glucose sulfate Glycan 0.5g / L, Proclin300 0.05%, R2: PIPES-Na 80mmol / L, peroxidase 2KU / L, sodium chloride 0.9g / L, BSA1.5g / L, sodium azide 2.0g / L, Polyoxyethylene alkylene tribenzyl phenyl ether 1g / L, the vol...

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Abstract

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a stabilizing agent for a high-density lipoprotein cholesterol detection reagent. The stabilizing agent is characterized by being prepared from gelatin, MgSO4, glycerol, glycine betaine, trehalose, polyethylene glycol, EDTA and polyoxyethylene alkylene tribenzyl phenyl ether. According to the stabilizing agent for the high-density lipoprotein cholesterol detection reagent, the valid period of the high-density lipoprotein cholesterol detection reagent can be significantly prolonged to two years, the stability can be kept for three months after a bottle containing the stabilizing agent is opened, a blank absorbency value is obviously decreased, reagent operation is easy, a result is accurate, and the stability is good.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a stabilizer for high-density lipoprotein cholesterol detection reagents with simple configuration, extended reagent validity period and high bottle-opening stability. Background technique [0002] As we all know, the main physiological function of high-density lipoprotein cholesterol is to transport phospholipids and cholesterol. It is an anti-atherosclerotic lipoprotein and a protective factor for coronary heart disease. The content of high-density lipoprotein cholesterol (HDL-C) is related to the arterial The degree of luminal stenosis is significantly negatively correlated. Clinically, the analysis of the ratio of different types of lipoproteins is used as the differential diagnosis of different types of hyperlipoproteinemia. Increased high-density lipoprotein cholesterol is commonly seen in: drinking, long-term physical activity, etc., high-density lipopr...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/44C12Q1/30C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/30C12Q1/44C12Q2326/96
Inventor 王新刚蔡润中付勇苟晓刚宫在科
Owner 威海威仕泰医疗科技有限公司
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