Tumor-associated antigen XAGE-1b short peptide and application thereof

Abstract

The invention discloses a tumor-associated antigen XAGE-1b short peptide and an application thereof. The sequence of the short peptide is selected from SEQ ID NO: 2-SEQ ID NO: 13. Cytotoxic T lymphocytes (CTLs) induced by the XAGE-1b antigen peptides do not generate immune response to testicular cells, and only kill tumor cell. The induced CTL clones have good tumor cell specific killing effect. Meanwhile, in the process of establishing the CTLs, the screened XAGE-1b antigen peptides are found to have appropriate affinity with HLA on DC cells and can effectively stimulate and induce the generation of specific CTLs, and it is proved that the XAGE-1b antigen peptides have good potential of a polypeptide vaccine and a DC vaccine. XAGE-1 b antigen peptide specific TCR genes are cloned, a virus carrier is constructed, the genes are transduced to CD8+T cells from peripheral circulation to obtain TCR gene modified T cell (TCR-T) clones, and also the specific killing effect on tumor cells similar to parental CTLs is obtained, and thus the tumor-associated antigen XAGE-1b short peptide has good clinical conversion and practical application prospect.

Description

technical field [0001] The invention relates to tumor-associated antigen peptides and applications thereof, in particular to tumor-associated antigen XAGE-1b short peptides and applications thereof. Background technique [0002] As we all know, T cell adoptive therapy for tumors is currently the most eye-catching technology, especially the application of receptor-engineered T cells is becoming an exciting cancer treatment method, and it also has broad application prospects for viral infections and autoimmune diseases. [0003] The human immune surveillance system plays an important role in the process of resisting tumor growth. The immune surveillance system achieves the purpose of inhibiting tumor growth by coordinating various types of immune cells, among which cytotoxic T cells (cytotoxic Tlymphocytes, CTLs) play an extremely important role and are the most important. Key killer effector cells. tumor antigen-specific CD8 + T cells can specifically recognize and directly...

AI Technical Summary

Problems solved by technology

In the technical scheme disclosed by CN102428102A, an attempt is made to intercept a part of the XAGE-1b antigen to induce humoral immunity or cellular immunity to the tumor, but the result is not satisfactory, and the length of the polypeptide is still not shorter than 16AA

[0013] Short peptides may also have the problem of poor specificity. How to obtain short peptides with good specificity and good immunogenicity is a very challenging task

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