A production system and preparation process suitable for yolk antibody

Abstract

The invention belongs to the field of preparing yolk antibodies and particularly discloses a production system and a preparing technology suitable for a yolk antibody. The production system comprises an egg beater, a yolk separating instrument, a yolk storage tank, a power pump I, a power pump II, a tank body I, an external auxiliary system of the tank body I, a tank body II, an external auxiliary system of the tank body II, a filter assembly, a vacuum freezing instrument and a finished product storage tank. The preparing technology comprises the first step of disinfection and cleaning treatment, the second step of egg breaking, the third step of yolk separation, the fourth step of temporary yolk storage, the fifth step of yolk diluting and disinfection, the sixth step of yolk liquid acidification layering and antibody extraction, the seventh step of filtering, the eighth step of vacuum freezing drying, and the ninth step of yolk antibody finished product storage. In the extracting and preparing process of the technology, the loss of the effective antibody is small, the obtained antibody is high in purity and titer, and the technology can be safely and conveniently used for industrial production and preparation of the yolk antibody.

Description

technical field [0001] The invention belongs to the field of yolk antibody production, and in particular relates to a production system and preparation process suitable for yolk antibody. Background technique [0002] Yolk antibodies (egg yolk antibodies, IgY) are immunoglobulins present in poultry yolks, and refer to specific antibodies produced by laying hens after being immunized with specific antigens, transferred and stored in egg yolks. IgY is extracted from egg yolks and It has been widely recognized in the prevention and treatment of animal diseases. Because of its good therapeutic effect, high specificity, safety and no residue, no drug-resistant bacteria, large amount of preparation, simple process and other advantages, it stands out in the research boom of antibiotic substitutes. Since it was first discovered by Klemperer in 1898, it has been widely used in the prevention and treatment of livestock and poultry diseases and disease diagnosis. The U.S. Food and Dr...

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Problems solved by technology

In recent years, scholars at home and abroad have reported many preparation methods of egg yolk antibody, but due to the improper selection and use of extraction equipment, especially the lack of suitable equipment for large-scale, industrialized and standardized antibody production in the current preparation of egg yolk antibody. The antibody extraction and post-purification process lead to partial loss of antibodies and lower titers. The result is: on the one hand, it increases the cost of antibody extraction; It directly leads to poor therapeutic effect of antibodies, seriously affects the effect of antibodies, and greatly limits the application of yolk antibodies in disease prevention and treatment

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