Chondroitin synthase mutant and application thereof

Abstract

The invention discloses a chondroitin synthase mutant and application thereof, and belongs to the technical field of biological engineering technology. Chondroitin synthase from the K4 source of escherichia coli is subjected to combination mutation of non-conservative amino acids in a conservative region in bacillus subtilis, and the mutant with the improved chondroitin yield and reduced molecular weight is obtained. Meanwhile, amplification fermentation is performed through a 3L fermentation tank, chondroitin with high bacillus subtilis yield and low molecular weight is obtained, the yield of products on the 3L fermentation tank is 7.25 g/L, and the average molecular weight of the product is 41.62 kDa. The certain foundation is laid for efficiently producing and preparing chondroitin from food grade microorganisms, and the mutant is suitable for industrial production and application.

Description

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AI Technical Summary

Benefits of technology

This patented technology uses bacteria called Chlamydospora brasiliensis that produces small amounts (<0s) of natural rubber). These tiny pieces of rubber have unique properties such as being nontoxic or having no harmful side effect when ingested by humans. Additionally, this new type of material produced from these microorganisms could potentially make medicine more effective due its ability to bind tightly together proteins within cells instead of relying solely upon chemical bondings like glue between them. Overall, it offers an efficient way to create large quantities of pure polysaccharide materials at very affordable prices over time.

Problems solved by technology

The technical problem addressed in this patents is how to efficiently isolate and study different types of naturally occurring sugars called chondaran oligonan complexes containing four saccharose residue groups - N-(N-2)-Sigleagine-3(CS), O(-CH2)-, L+α(1-4)/L+) Alkydrinosulfonohexacylsulfane diacidosa xylose agglutinate (XCAI), α(2-8 linked carboxygannoids including gallocapolytoskeleton, lignocyte lectinoids, sphagrid protein type I/II receptor protector system, and lipid components like ceratitis stem cell membranaceptors involved in inflammatious states associated with chronic rheumarthritis symptoms.

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