52 results about "Non conservative" patented technology

Novel antagonists of CXCR3-binding CXC chemokines, and in particular of human CXCL11, can be obtained by generating mutants of such chemokines in which the binding to glycosaminoglycans (GAGs) is impaired due to non-conservative substitutions of amino acids involved in this interaction. Compounds prepared in accordance with the present invention can be used to block the activity of CXCR3-binding CXC chemokines on CXCR3-expressing cells, thereby providing therapeutic compositions for use in the treatment or prevention of diseases related to excessive activated T cells migration, such as graft rejection and autoimmune diseases, and of diseases needing an increase of vascularization, such as ischemic heart disease.

According to one or more aspects, systems and techniques for hydrogen fueling with integrity checks are provided herein. Communicated parameters measured by on-board sensors of a vehicle may be cross-referenced against calculated parameters measured by sensors of a fueling station. For example, communicated parameters relating to a compressed hydrogen storage system (CHSS) tank of a vehicle to be fueled may be received at different time intervals. Calculated parameters may be calculated based on a mass of hydrogen fuel dispensed by a hydrogen fueling station from a reference point to one of the time intervals and densities of the CHSS tank of the vehicle at respective time intervals. An error may be calculated between the communicated parameters and the calculated parameters. A fueling mode, such as a conservative fueling mode or a non-conservative fueling mode, may be determined based on the calculated error.

In some embodiments, the illustrative method defines an engine cycle comprising several state changes that allow for a net gain of energy from an underlying source force field. The potential for a net energy gain via the method results from the discovery that a Casimir force system can be rendered non-conservative. This is done by appropriately altering one or more of a variety of physical factors that affect the Casimir force, or by altering any of a variety of environmental factors that affect such physical factors. In various embodiments, the extracted energy is stored, used to power energy-consuming devices or used to actuate a micromechanical device. In one embodiment, the method is implemented using an energy extraction apparatus that comprises two spaced Casimir force-generating boundaries that are operatively coupled to an energy transformation system. The energy transformation system includes a first device that is operable to alter at least one physical factor of the system. The energy transformation system also includes a second device that is operable to change the distance between the two Casimir force-generating boundaries, and further operable to maintain the distance between the boundaries while the first device alters the physical factor.

Computer-based computational tools for use in determining spatial charge distributions for biological systems that include one or more biological membranes are provided. At least one of the biological membrane includes at least two regions having different electrical properties, e.g., the biological membrane can include a pore having a higher conductivity than the surrounding bulk membrane. In other cases, the membrane can include non-active and active regions, with conservative fields acting at the non-active regions and a combination of conservative and non-conservative fields acting at the active regions. The non-conservative fields can, for example, originate from differences in ionic concentrations of the type which generate Nernst potential differences across membranes. Using the computer-based computational tools, charge distributions not previously known to exist have been discovered, e.g., ring-shaped charge distributions in the vicinity of an active pore.

Heteroclitic analogs of class I epitopes are prepared by providing conservative, semi-conservative, or non-conservative amino acid substitutions at positions 3 and / or 4 and / or 5 and / or 6 and / or 7 and / or 8 and / or 9 and / or 10 of these epitopes. The analogs are useful in eliciting immune responses with respect to the corresponding wild-type epitopes.

A high-precision magnetic suspension accelerometer for measuring the linear acceleration of a spacecraft is provided, comprising a magnetically shielded vacuum chamber system, a magnetic displacement sensing system, a magnetic suspension control system and a small magnetic proof mass. A optical coherence displacement detection technique is utilized for precisely measuring the position and the posture of the small magnetic proof mass in real time, and a magnetic suspension control technique is utilized for precisely controlling the position and the posture of the small magnetic proof mass to be brought back to the origin, so as to keep the small magnetic proof mass in the center of the systemic inner chamber. When the spacecraft is subject to a non-conservative force, the magnitude and direction of the acceleration can be precisely measured via the measurement of currents in the position control coils due to the acceleration of the spacecraft proportional to the currents of the position control coils. The accelerometer of the invention can avoid the technical bottleneck of high-precision machining, is easy to be produced and can achieve more high-precision measurement of the acceleration vector.

In some embodiments, the illustrative method defines an engine cycle comprising several state changes that allow for a net gain of energy from an underlying source force field. The potential for a net energy gain via the method results from the discovery that a Casimir force system can be rendered non-conservative. This is done by appropriately altering one or more of a variety of physical factors that affect the Casimir force, or by altering any of a variety of environmental factors that affect such physical factors. In various embodiments, the extracted energy is stored, used to power energy-consuming devices or used to actuate a micromechanical device. In one embodiment, the method is implemented using an energy extraction apparatus that comprises two spaced Casimir force-generating boundaries that are operatively coupled to an energy transformation system. The energy transformation system includes a first device that is operable to alter at least one physical factor of the system. The energy transformation system also includes a second device that is operable to change the distance between the two Casimir force-generating boundaries, and further operable to maintain the distance between the boundaries while the first device alters the physical factor.

The invention discloses a method for obtaining drain basin unit lines by utilizing complete two-dimensional shallow water equation sets. According to the method, a hydrological problem is solved by utilizing a hydrodynamic way. The method comprises the following steps of: firstly discretizing a two-dimensional shallow water equation set to establish a high-resolution two-dimensional shallow watermathematic model, so as to ensure that the two-dimensional shallow water model is capable of correctly simulating slope runoff processes; carrying out elevation assignment and roughness factor assignment by adoption of a triangular unstructured grid division calculation area; in order to avoid the problem of non-conservative drainage basin outlet water yield caused by drainage basin depression impounding, setting continuous time interval net rainfall processes as model input conditions; setting a drainage basin outlet as a free falling outflow condition; carrying out two-dimensional shallow water mathematic model calculation after setting a drainage basin initial state; after the calculation is ended, enabling a flow process at the drainage basin outlet as an S curve of the drainage basin;and obtaining a time interval unit line of the drainage basin by utilizing a conversion formula. The method is capable of covering the shortages of existing hydrological methods, and providing a newway for flood confluence analysis and calculation of regions without hydrological data.

The present invention relates to the application of the biological adsorption and biological oxidation ability of both conventional wastewater treatment biological selection technology and, if desired, chemically-enhanced wastewater treatment biological selection technology to remove pollutants from precipitation-generated flows collected by a combined sanitary sewer system, a separate sanitary sewer system, and / or a combination of both systems for: (1) the biological adsorption and oxidation of suspended and dissolved pollutants as measured by standard methods tests for carbonaceous biochemical oxygen demand, and / or (2) the biological adsorption of all other dissolved non-conservative and conservative pollutants as measured by all other standard methods tests for such pollutants.

The invention is directed to a method of treating movement disorders by the modulation of neuronal transmission using time-variant non-conservative magnetic fields. The invention is also directed to a method for treating dystonias.

Nucleic acid sequences encoding improved Herpes Simplex Virus Thymidine Kinases are provided, including their use in diagnostic and therapeutic applications. The thymidine kinases may be mutated using conservative mutations, non-conservative mutations, or both. Also provided are gene therapeutic systems, including viral and retroviral particles.

The invention discloses a method for thermal radiation force modeling of a navigation satellite complex model. The method comprises the steps of establishing a data information base of surface components generating thermal radiation force by obtaining a surface three-dimensional model of a to-be-analyzed satellite; analyzing thermal radiation force of the working conditions of the components under a body coordinate system, and calculating the thermal radiation force and thermal radiation force acceleration by vector synthesis of the thermal radiation force of the surface components so as to establish a mathematical model of the thermal radiation force and the thermal radiation force acceleration. The high-precision thermal radiation force model can be established through the modeling method, and the thermal radiation force serving as non-conservative force can improve a kinetic model of the high-precision satellite and further improve orbit determination precision and orbit prediction precision.

The invention discloses DNA polymerase for improving the synthesis efficiency of catalytic DNA and belongs to the technical field of biological engineering. The DNA polymerase is prepared by the following steps of: firstly mutating the 241-245th amino acids KSKIP of Dhb into RVRKS, or mutating the 250th-site L into V, or mutating the 221th-site A into S, or mutating the 76-site M into I, then fusing Sso7d by flexible linker at the N end of a mutant and constructing out Dbh with improvement of the nucleotide doping efficiency, i.e., four positive mutants such as SdhbM76I, Sdbh A221S, Sdbh KSKIP(241-245)RVRKS and Sdbh L250V. Simultaneously, the invention also provides a method for enhancing the continuous synthesis capability by non-conservative sites of a mutant enzyme.

Novel antagonists of CXCR3-binding CXC chemokines, and in particular of human CXCL11, can be obtained by generating mutants of such chemokines in which the binding to glycosaminoglycans (GAGs) is impaired due to non-conservative substitutions of amino acids involved in this interaction. Compounds prepared in accordance with the present invention can be used to block the activity of CXCR3-binding CXC chemokines on CXCR3-expressing cells, thereby providing therapeutic compositions for use in the treatment or prevention of diseases related to excessive activated T cells migration, such as graft rejection and autoimmune diseases, and of diseases needing an increase of vascularization, such as ischemic heart disease.

The present disclosure provides humanized CC49 monoclonal antibodies that bind TAG-72 with high binding affinity and that are minimally immunogenic. In one embodiment, a humanized CC49 antibody includes a non-conservative amino acid substitution in a light chain complementarity determining region 3 of the CC49 antibody. In a further embodiment, the humanized CC49 antibody includes a non-conservative substitution of a first residue in a light chain complementarity determining region 3 and a substitution of a second residue in a complementarity determining region of the humanized CC49 antibody. In several of the embodiments, methods are disclosed for the use of a humanized CC49 antibody.

The present invention provides an isolated tyrosyl tRNA synthetase (TyrRS) polypeptide variant which comprises (a) a Rossmann fold region or a portion thereof, preferably including an alpha5 coil; and (b) an anticodon recognition domain or portion thereof, preferably including an alpha14 coil. Preferably, the alpha5 coil and the alpha14 coil have a greater spatial separation in the tertiary structure of the variant compared to the corresponding spatial separation in native human TyrRS. The variant preferably comprises an amino acid residue sequence identity of at least about 50 % compared to the amino acid residue sequence of human TyrRS (SEQ ID NO: 3), includes at least one non-conservative amino acid residue substitution relative to the amino acid residue sequence of human TyrRS, and preferably presents an exposed ELR motif in the alpha5 coil on an external portion of the tertiary structure of the polypeptide. A preferred TyrRS protein variant comprises the amino acid residue sequence of SEQ ID NO: 4 or a portion thereof. The proteins and protein fragments of the invention are angiogenic and are useful for stimulating angiogenesis in mammalian tissues.

Nucleic acid sequences encoding improved Herpes Simplex Virus Thymidine Kinases are provided, including their use in diagnostic and therapeutic applications. The thymidine kinases may be mutated using conservative mutations, non-conservative mutations, or both. Also provided are gene therapeutic systems, including viral and retroviral particles.

The invention discloses a water age measurement method for a semi-closed water area. The method comprises the following steps that: firstly, establishing the hydrodynamic force mathematical model of awater area to be measured; through the hydrodynamic force mathematical model, calculating the water depth and flow velocity information of each water quality point of the water area to be measured; constructing a convective diffusion mathematical model, and setting the boundary condition of the convective diffusion mathematical model; according to the hydrodynamic force mathematical model, calculating to obtain the water level and flow velocity information of each position of the area to be measured and the boundary condition of the convective diffusion mathematical model; calculating the concentration values of a conservative substance p and a non-conservative substance r of each moment of each water quality point of the area to be measured through the convective diffusion mathematical model; according to the concentration values of a conservative substance p and a non-conservative substance r of each moment of each water quality point of the area to be measured, calculating the instantaneous water age of each corresponding moment of each water quality point of the area to be measured; and finally, according to the instantaneous water age of each corresponding moment of each water quality point of the area to be measured, calculating a time average water age in each time period of each water quality point of the water area to be measured. By use of the method, the water age of the semi-closed water area can be efficiently, conveniently and accurately calculated.

Novel antagonists of the chemokine CXCL8 (also known as Interleukin-8) can be obtained by generating mutants having specific combinations of non-conservative substitutions of basic amino acids located in the C-terminal region. Compounds prepared in accordance with the present invention can be used to block CXCL8 activity in vivo, thereby providing therapeutic compositions for use in the treatment or prevention of CXCL8-related diseases.

Mutants of specific CC-chemokines containing a non-conservative substitution in a conserved consensus sequence act as CC-chemokine antagonists, and can be effectively used in the treatment of autoimmune and inflammatory diseases, cancers, and viral or bacterial infections. Particularly preferred are the RANTES / CCL5 mutants having the amino acid sequence of SEQ ID NO: 1, 2, 3, or 4.

The present invention relates to a plant having an improved sugar content in fruit compared with its wild type plant, which has a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein. The present invention also relates to a parthenocarpic plant having a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein.

The invention discloses a method for obtaining drain basin unit lines by utilizing complete two-dimensional shallow water equation sets. According to the method, a hydrological problem is solved by utilizing a hydrodynamic way. The method comprises the following steps of: firstly discretizing a two-dimensional shallow water equation set to establish a high-resolution two-dimensional shallow watermathematic model, so as to ensure that the two-dimensional shallow water model is capable of correctly simulating slope runoff processes; carrying out elevation assignment and roughness factor assignment by adoption of a triangular unstructured grid division calculation area; in order to avoid the problem of non-conservative drainage basin outlet water yield caused by drainage basin depression impounding, setting continuous time interval net rainfall processes as model input conditions; setting a drainage basin outlet as a free falling outflow condition; carrying out two-dimensional shallow water mathematic model calculation after setting a drainage basin initial state; after the calculation is ended, enabling a flow process at the drainage basin outlet as an S curve of the drainage basin;and obtaining a time interval unit line of the drainage basin by utilizing a conversion formula. The method is capable of covering the shortages of existing hydrological methods, and providing a newway for flood confluence analysis and calculation of regions without hydrological data.

The oral efficacy of C—C chemokines containing a dibasic site in the 40's conserved cationic sequence, such as RANTES and MIP-1β, is improved by substituting at least one of the residues in the dibasic site in a non-conservative manner.

The invention relates to a method for changing the conservative internal force as gravity into non-conservative internal force, and relative device. It can solve the problem that the conservative internal force can not make power. Based on the Newton theory, the mechanical energy conservation theory, and the law of conservation of momentum, via mechanical energy storage, transmission, inverse releasing and layering, it changes the conservative internal force into the non-conservative internal force which can make power. Wherein, the inventive device, is a disc element (2) rotating around a axle (1), whose two surfaces have several uniformly distributed grooves (3) which are inclined from inner to outer (or from outer to inner) and from upper to lower; and each groove comprises a ball (or cylinder) (4) which can roll freely; and one end of each groove has a kinetic energy storing device (5) formed by a spring, a piston and a stop pin. The invention can be used to drive the machine with lower rotational speed and drive the generator.

Mutants of specific CC-chemokines containing a non-conservative substitution in a conserved consensus sequence act as CC-chemokine antagonists, and can be effectively used in the treatment of autoimmune and inflammatory diseases, cancers, and viral or bacterial infections. Particularly preferred are the RANTES / CCL5 mutants having the amino acid sequence of SEQ ID NO: 1, 2, 3, or 4.

The invention relates to the field of rice utilization, in particular to a molecular marking method used for rice cytoplasm identification. The molecular marking method comprises the steps that CW, Lead, Nipponbare and 9311 mitochondria genomes are analyzed and compared, and non-conservative MS sequences are screened out; corresponding molecular marking PCR primers are designed according to the MS sequences; total DNA of different kinds of rice is extracted; the total DNA is used as a template, PCR amplification is conducted with the molecular marking PCR primers as primers and amplification products are obtained; electrophoresis detection is conducted on the amplification products, the polymorphism primers causing amplification differences are screened out and target strips obtained by amplifying the polymorphism primers are obtained; the target strips obtained by amplifying the polymorphism primers are counted, and a cluster graph is established so as to conduct genetic relationship analysis. The molecular marking method used for rice cytoplasm identification is simple and convenient to operate, simple in needed equipment and reliable and stable in result.

The present invention relates to the field of Biotechnology, particularly to a method based on the introduction of a single mutation in the genes encoding the human IL-2 and the muteins derived thereof that results in increased secretion levels in different hosts without affecting their biological functions. In particular, these mutations are based on a non-conservative change in the amino acid located in position 35 in the primary sequence of human IL-2, preferably the substitutions are K35E, K35D and K35Q. Another object of the present invention are the expression systems used to obtain both the recombinant human IL-2 and the muteins derived thereof using the method described in this invention. The above-mentioned method is useful to improve the production efficiency of the recombinant human IL-2 and the muteins derived thereof both at laboratory and industrial scales. The proteins obtained using this method can be used for therapeutic purposes as well as in the in vitro expansion of T cells for adoptive transfer therapies.

According to one or more aspects, systems and techniques for hydrogen fueling with integrity checks are provided herein. Communicated parameters measured by on-board sensors of a vehicle may be cross-referenced against calculated parameters measured by sensors of a fueling station. For example, communicated parameters relating to a compressed hydrogen storage system (CHSS) tank of a vehicle to be fueled may be received at different time intervals. Calculated parameters may be calculated based on a mass of hydrogen fuel dispensed by a hydrogen fueling station from a reference point to one of the time intervals and densities of the CHSS tank of the vehicle at respective time intervals. An error may be calculated between the communicated parameters and the calculated parameters. A fueling mode, such as a conservative fueling mode or a non-conservative fueling mode, may be determined based on the calculated error.

Computer-based computational tools for use in determining spatial charge distributions for biological systems that include one or more biological membranes are provided. At least one of the biological membrane includes at least two regions having different electrical properties, e.g., the biological membrane can include a pore having a higher conductivity than the surrounding bulk membrane. In other cases, the membrane can include non-active and active regions, with conservative fields acting at the non-active regions and a combination of conservative and non-conservative fields acting at the active regions. The non-conservative fields can, for example, originate from differences in ionic concentrations of the type which generate Nernst potential differences across membranes. Using the computer-based computational tools, charge distributions not previously known to exist have been discovered, e.g., ring-shaped charge distributions in the vicinity of an active pore.