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Doxorubicin hydrochloride magnetic nanoparticles and preparation method thereof

A technology of doxorubicin hydrochloride and magnetic nanoparticles, which is applied in the direction of pharmaceutical formulations, organic active ingredients, and medical preparations of non-active ingredients, etc., can solve problems such as inability to achieve drug delivery, high antibody prices, and immune exclusion reactions. Increased encapsulation efficiency and drug loading, stable properties, and high drug loading

Active Publication Date: 2017-01-04
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most antibodies are confined to the cell membrane, unable to achieve intracellular delivery of drugs, and at the same time easily cause immune exclusion reactions in the body, coupled with the high price of antibodies, greatly restricting the application of antibodies

Method used

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  • Doxorubicin hydrochloride magnetic nanoparticles and preparation method thereof
  • Doxorubicin hydrochloride magnetic nanoparticles and preparation method thereof
  • Doxorubicin hydrochloride magnetic nanoparticles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Optimization of preparation conditions for magnetic drug-loaded nanoparticles

[0044] 1. Investigation of preparation method

[0045] Nanoparticles can be prepared by rotary evaporation, dialysis and rotary evaporation dialysis. The rotary evaporation method is to drop the solution mixed with the drug and nanomaterials into water, and stir at a certain speed to obtain a micellar solution. In the dialysis method, the drug and nano-blocks are directly loaded into the dialysis bag, and the nano-micelle solution is obtained through dialysis. The rotary evaporation dialysis method is to combine the two, the rotary evaporation method is the same as the above, the solution obtained by the rotary evaporation is put into a dialysis bag, and dialyzed in water to obtain a micellar solution. The advantage of the rotary evaporation method is that the reaction is more thorough and purer nanoparticles are obtained.

[0046] The nanoparticles prepared by different method...

Embodiment 2

[0064] Embodiment 2: Preparation of magnetic drug-loaded nanoparticles

[0065] Accurately weigh 20mg of polymer PEG-PLA, dissolve in 4ml dimethylformamide to make solution A; accurately weigh 10mg of doxorubicin hydrochloride (protect from light), dissolve in 4ml dimethylformamide to make solution B; Add 20ul triethylamine to solution B, deionize doxorubicin hydrochloride, 30-40W, ultrasonication for 10min; accurately weigh 10mg of ferric oxide powder, dissolve in 4ml tetrahydrofuran, and ultrasonicate at 200W for 30min to make solution C; Pour the solution into a beaker, and under stirring with a magnetic stirrer, add the deionized B solution dropwise to the A solution at a rate of 20-30 drops / min; Mix the mycin and the nano-block evenly, and the organic solvent volatilizes; slowly add the C solution dropwise to the above-mentioned drug-loaded nano-micelle, and stir evenly with a glass rod; put the prepared magnetic drug-loaded nano-micelle solution into a dialysis bag for d...

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Abstract

The invention discloses a preparation method of doxorubicin hydrochloride magnetic nanoparticles. The preparation method comprises the following steps: (1) dissolving nano block polymer polyethylene glycol-polylactic acid in dimethyl formamide to prepare a solution A; (2) dissolving doxorubicin hydrochloride in dimethyl formamide to prepare a solution B; (3) adding triethylamine into the solution B, and performing the deionization ultrasonic dissolving on the doxorubicin hydrochloride; (4) dissolving ferroferric oxide powder in tetrahydrofuran, and ultrasonically dispersing to obtain a solution C; (5) dropwise adding the deionized solution B into the solution A under a stirring condition; (6) stirring and uniformly mixing the doxorubicin hydrochloride and nano blocks, and volatilizing organic solvent to obtain medicine carrying micelle; (7) dropwise adding the solution C into the medicine carrying nano micelle obtained in the step (6), and uniformly stirring; (8) dialyzing to obtain magnetic medicine carrying nano micelle; and (9) adding a freeze drying protective agent into the nano micelle to obtain the magnetic medicine carrying nanoparticles. The method is simple in operation procedures and easy in operation.

Description

technical field [0001] The invention relates to a doxorubicin hydrochloride magnetic nano particle and a preparation method thereof. Background technique [0002] Doxorubicin hydrochloride is an anthracycline anti-cancer drug commonly used in clinical practice. Its mechanism of action is mainly that doxorubicin hydrochloride can be embedded in RNA and DNA molecules to inhibit the synthesis of nucleic acids, thereby producing anti-tumor effects. Doxorubicin hydrochloride can produce a wide range of biochemical effects on the body. Doxorubicin hydrochloride is widely used in the treatment of acute leukemia, sarcoma, non-Hodgkin's lymphoma, Multiple myeloma, breast, ovarian and cervical cancer. Doxorubicin hydrochloride increases HCL on the surface of doxorubicin to change the water solubility of doxorubicin, but its molecular structure is unstable, and it is prone to changes such as hydrolysis and photolysis, which reduces its curative effect. And after intravenous injectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K41/00A61K31/704A61K47/34A61K47/02A61K47/10A61P35/00
CPCA61K9/0009A61K9/1075A61K31/704A61K41/00A61K47/02A61K47/10A61K47/34
Inventor 鲁在君宋坤孔北华姚舒苑存忠
Owner SHANDONG UNIV QILU HOSPITAL
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