Externally-applied medicine for treating cutaneous appendages and skin pruritic disease and preparation method and application thereof
A technology for pruritic skin diseases and external use of drugs, applied in the field of biopharmaceuticals, can solve the problems of long course of treatment, single treatment, toxic and side effects, etc., and achieve the effects of rapid curative effect, improvement of immune system, and skin repair.
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[0028] Example 1
[0029] 1. Material:
[0030] The methods used in this embodiment are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used, unless otherwise specified, are all commercially available products.
[0031] 2. Method
[0032] 2.1 Preparation of NK cell stock solution
[0033] Step 1: PBMC extraction:
[0034] Centrifuge the fresh blood at 740g for 10 minutes to obtain the upper layer of light yellow plasma and the lower layer of blood cells. Mix the lower layer of blood cells with saline in a volume ratio of 1:1, take a 50ml centrifuge tube, and spread the diluted blood cell suspension on 15ml of lymphocyte separation solution Centrifuge at 400g for 30 minutes. The middle white membranous cell layer was collected, washed with physiological saline, centrifuged at 400g for 10 minutes, the supernatant was discarded, and the washing was repeated once. Finally, resuspend the cell pellet with an appropriate...
Example Embodiment
[0065] Example 2
[0066] This embodiment provides a SNK solution, by weight, the SNK solution is composed of 70.0% of NK cell stock solution, 5.0% of glycerin, 0.03% of ethyl p-hydroxybenzoate, 0.08% of borax, 1.5% of peppermint oil and an appropriate amount of sterile Water composition, wherein the preparation method of the NK cell stock solution is the same as in Example 1.
[0067] Select 3 patients with acne vulgaris and 2 patients with pruritus, spray the above-mentioned SNK liquid directly on the affected area for treatment, use it 2-3 times a day, and count the clinical treatment results.
[0068] The results showed that the above-mentioned SNK solution has a significant therapeutic effect on acne vulgaris. The symptoms of severe patients are significantly reduced within 7 days and can be cured after 2 weeks of continuous use; it has a certain therapeutic effect on pruritus, and it can be cured within 2 weeks for mild patients and 2 for severe patients Symptoms improved sign...
Example Embodiment
[0069] Example 3
[0070] This embodiment provides a SNK cream, by weight, the SNK cream is composed of 60.0% of NK cell stock solution, 0.5% of carbomer 941, 5.0% of glycerin, 0.5% of aloe vera powder, and 0.5% of ethyl p-hydroxybenzoate. , Hyaluronic acid 0.3%, peppermint oil 1.5% and appropriate amount of water.
[0071] Select 2 patients with seborrheic dermatitis, apply the above-mentioned SNK ointment for smear treatment, use it 2-3 times a day, and count the clinical treatment results.
[0072] The results showed that after 2 weeks of use, the symptoms of the two patients with seborrheic dermatitis were significantly improved, indicating that the SNK cream has a certain therapeutic effect on seborrheic dermatitis.
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