Lyceum barbarum polysaccharide extract, extraction method and application of lycium barbarum polysaccharide extract in preparation of blood fat reducing drugs
A technology of Lycium barbarum polysaccharide and extraction method, which is applied in the directions of drug combination, medical formula, plant raw material, etc., can solve the problems of low purity and low polysaccharide activity, and achieve the effect of good blood lipid lowering application effect.
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Embodiment 1
[0025] The extraction method of wolfberry polysaccharide extract comprises the following steps:
[0026] Step 1, in parts by weight, take 200 parts of dried wolfberry, add to 100 parts of petroleum ether, stir evenly, let stand for 10 minutes, filter with suction, dry the solid in a 40°C decompression drying oven for 1 hour, Obtain the first wolfberry powder;
[0027] In the second step, the first wolfberry powder is subjected to 0.5Mpa high-pressure treatment in the range of 20°C to obtain the second wolfberry powder;
[0028] Step 3, add the second wolfberry powder into distilled water, control the ratio of material to liquid to 1g / 30ml, then add 0.1% enzyme to the material liquid, carry out enzymatic hydrolysis and extraction, and the enzyme is selected from alkaline pectate cleavage The mixture of enzyme, alkaline cellulase, alkaline xylanase and laccase according to the weight ratio of 2:3:2:0.5, the temperature range of enzymolysis and extraction at 60°C, the time is 2h...
Embodiment 2
[0033] The extraction method of wolfberry polysaccharide extract comprises the following steps:
[0034] Step 1, in parts by weight, take 220 parts of dried wolfberry, add to 120 parts of petroleum ether, stir evenly, let stand for 20 minutes, filter with suction, dry the solid in a 60°C decompression drying oven for 3 hours, Obtain the first wolfberry powder;
[0035] In the second step, the first wolfberry powder is subjected to 1Mpa high-pressure treatment in the range of 40°C to obtain the second wolfberry powder;
[0036] Step 3, add the second wolfberry powder into distilled water, control the ratio of material to liquid to 1g / 35ml, and then add 0.2% enzyme to the material solution for enzymatic hydrolysis and extraction. The enzyme is selected from alkaline pectate cleavage The mixture of enzyme, alkaline cellulase, alkaline xylanase and laccase according to the weight ratio of 2:3:2:0.5, the temperature range of enzymolysis and extraction at 70°C is 4h, and it is coll...
Embodiment 3
[0041] The extraction method of wolfberry polysaccharide extract comprises the following steps:
[0042] Step 1, in parts by weight, take 210 parts of dried wolfberry, add to 110 parts of petroleum ether, stir evenly, let stand for 15 minutes, filter with suction, dry the solid in a 45°C vacuum drying oven for 2 hours, Obtain the first wolfberry powder;
[0043] In the second step, the first wolfberry powder is subjected to 0.8Mpa high-pressure treatment in the range of 30°C to obtain the second wolfberry powder;
[0044] Step 3, add the second wolfberry powder into distilled water, control the ratio of material to liquid to 1g / 32ml, then add 0.15% enzyme to the material solution, perform enzymatic hydrolysis and extraction, and the enzyme is selected from alkaline pectate cleavage The mixture of enzyme, alkaline cellulase, alkaline xylanase and laccase according to the weight ratio of 2:3:2:0.5, the temperature range of enzymolysis and extraction at 65°C, the time is 3h, col...
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