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Stabilizer for low density lipoprotein cholesterol test reagent

A low-density lipoprotein and stabilizer technology, applied in the field of in vitro diagnostic reagents, can solve problems affecting clinical use, interference, and poor stability of detection reagents, and achieve the effects of good stability, accurate results, and extended validity period

Active Publication Date: 2017-01-04
威海威仕泰医疗科技有限公司
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Problems solved by technology

[0003] At present, low-density lipoprotein cholesterol detection kits mostly use the direct method-surfactant scavenging method, which is widely used clinically due to its high sensitivity, high accuracy, high precision and applicability, but because the enzyme activity will be affected The interference of PH, temperature, and chemical reagents leads to the problem of poor stability of the detection reagents, which affects clinical use. 8°C, the validity period is 12 months; the reagent is stored at 2~8°C in the dark after opening, and the validity period is 1 month under the condition of no pollution

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  • Stabilizer for low density lipoprotein cholesterol test reagent
  • Stabilizer for low density lipoprotein cholesterol test reagent
  • Stabilizer for low density lipoprotein cholesterol test reagent

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[0013] A stabilizer for low-density lipoprotein cholesterol assay reagent, characterized in that the stabilizer consists of gelatin, MgSO 4 , glycerin, betaine, trehalose, polyethylene glycol, EDTA, its weight components are: gelatin 1 ~ 10ml / L, MgSO 4 0.1~5g / L, glycerin 1~15ml / L, betaine 0.1~1mol / L, trehalose 0.1g / L~20 g / L, polyethylene glycol 0.5~2g / L, EDTA 0.5~2.0g / L , the low-density lipoprotein cholesterol detection reagent is composed of reagent 1 and reagent 2: R1: PIPES-Na 80mmol / L, cholesterol esterase 2KU / L, cholesterol oxidase 2KU / L, catalase 200LU / L, 4-aminoantipyrine 200mmol / L, trimethyl-β-cyclodextrin 5g / L, ethylene oxide octadecylamine 3g / L, potassium ferrocyanide 50μmol / L, chloride Sodium 25ml / L, Proclin300 0.05%, R2: PIPES-Na 80mmol / L, peroxidase 2KU / L, sodium chloride 0.9g / L, BSA 1.5g / L, sodium azide 2.0g / L, TritonX- 100 18g / L, the volume of the stabilizer used in the reagent is 5 ml / L, the pH of the reagent is 6.8-7.0, this pH value is cholesterol ester...

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Abstract

The invention relates to the technical field of in vitro diagnostic reagents, in particular to a stabilizer for a low density lipoprotein cholesterol test reagent. The stabilizer is characterized by being prepared from gelatin, MgSO4, glycerol, betaine, trehalose, polyethylene glycol and ethylene diamine tetraacetic acid (EDTA). The stabilizer for the low density lipoprotein cholesterol test reagent can remarkably prolong the validity period of the low density lipoprotein cholesterol test reagent to 2 years and maintain stability of the low density lipoprotein cholesterol test reagent in an opened bottle for 3 months, and obviously reduces blank absorbency; the reagent is simple in operation, accurate in results and good in stability.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a stabilizer for low-density lipoprotein cholesterol assay reagents with simple configuration, extended reagent validity period and high bottle-opening stability. Background technique [0002] It is well known that LDL cholesterol is used in the diagnosis of coronary heart disease and atherosclerosis. The lipids deposited in atherosclerotic (AS) plaques are mainly low-density lipoprotein (LDL). Among various lipids, LDL is considered to be the main pathogenic factor, while high-density lipoprotein (HDL) may Plays a protective role, the serum total cholesterol (TC) is large, which reflects the level of LDL cholesterol (LDL-CHO), but is also affected by the level of HDL-C. Therefore, in the identification of AS lipid risk factors, when TC is high, LDL is measured -C has important clinical significance. [0003] At present, low-density lipoprotein cholesterol ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/30C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/30C12Q1/44C12Q1/60C12Q2326/96
Inventor 王新刚蔡润中付勇苟晓刚宫在科
Owner 威海威仕泰医疗科技有限公司
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