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Fluorescence enhancement structure and system based on U-shaped metal array structure

A fluorescence enhancement, metal array technology, applied in the field of optoelectronics, can solve the problems of complex preparation, fluorescent molecule pollution, and the enhancement effect of fluorescence radiation needs to be improved, and achieves the effect of simple structure preparation process, ensuring no pollution, and promoting application and development.

Inactive Publication Date: 2019-01-18
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, these methods are complicated to prepare to a certain extent, the introduction of other substances will pollute the fluorescent molecules themselves, and the enhancement effect of fluorescent radiation needs to be improved.

Method used

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  • Fluorescence enhancement structure and system based on U-shaped metal array structure
  • Fluorescence enhancement structure and system based on U-shaped metal array structure
  • Fluorescence enhancement structure and system based on U-shaped metal array structure

Examples

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Embodiment 1

[0031] The preparation process of the fluorescence-enhanced structure in Example 1 is as follows:

[0032] 1) Evaporate a layer of 30nm thick gold film on the glass substrate, and the thickness value is optimized;

[0033] 2) Obtain a U-shaped metal structure arranged in a periodic array by focusing ion beam etching on the gold-plated film. Such as figure 1 As shown, the optimized parameters of a single U-shaped metal structure are: the length of the base is s1=150nm, the length of the left and right arms s2=140nm, the width of the left and right arms w=45nm, the depth of the arms d=70nm, the thickness of the U-shaped metal structure, that is, gold The film thickness h=40nm, the lateral period is 230nm, and the vertical period is 240nm. For the convenience of microscope observation, the engraved U-shaped metal structure array ranges from 16 μm×17 μm, such as figure 2 shown.

[0034] 3) Mix 20mg / mL polyvinyl alcohol aqueous solution and 2mmol / L Rhodamine 800 aqueous soluti...

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Abstract

The invention discloses a U-shaped metal array structure based fluorescence enhancement structure and system. A fluorescence enhancement method by utilizing the U-shaped metal structure capable of generating high-order magnetic resonance is provided for the first time, a glass substrate is plated with a metal film to prepare the U-shaped metal array structure, and the high-order magnetic resonance excited by the structure is optimized, so that the resonance peak is close to the radiation band of the fluorescent molecule, and the radiation enhancement of fluorescent molecules on the surface of the structure is realized. The intensity of the fluorescence radiation near the resonant wavelength can be enhanced to be several times of the intensity of the fluorescence radiation on the surface of the substrate, and the fluorescence radiation effect is greatly enhanced, so that a great promotion role can be played on development of the fluorescence enhancement technology.

Description

technical field [0001] The invention belongs to the field of optoelectronics, in particular to a fluorescence enhancement structure and system based on a U-shaped metal array structure. Background technique [0002] In the field of optoelectronics, the use of metal surface plasmon resonance can significantly enhance the fluorescence radiation intensity of fluorescent molecules distributed near the surface. This method of fluorescence enhancement is widely used in optical sensing, biological imaging, fluorescent detection labeling and other fields. At present, the more commonly used surface fluorescence enhancement technology uses a special surface shape, metal particle clusters to generate strong surface plasmon resonance, or a variety of chemical groups to modify the surface, change the physical or chemical properties of the surface, and make the fluorescence radiation Increased strength. However, the preparation of these methods is complicated to a certain extent, the in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/648
Inventor 李强罗丝仇旻
Owner ZHEJIANG UNIV
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