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A method for detecting chlorophyll a content in tea using confocal Raman microscopy

A technology of confocal microscopy and chlorophyll, which is applied in Raman scattering, material excitation analysis, etc., can solve the problems of unstable chlorophyll properties, destructiveness, cumbersome detection steps, and reduced accuracy of chlorophyll, achieving good economic benefits, The effect of shortening the detection time, simple structure and principle

Active Publication Date: 2019-04-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional detection methods are destructive, the detection steps are cumbersome, and online detection cannot be realized
At the same time, the properties of chlorophyll in tea are not very stable, and are easily changed by light, oxygen, and heat during the experiment. The accuracy of traditional chemical methods for determining chlorophyll in tea is easily affected by experimental conditions and experimental operations.

Method used

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  • A method for detecting chlorophyll a content in tea using confocal Raman microscopy
  • A method for detecting chlorophyll a content in tea using confocal Raman microscopy
  • A method for detecting chlorophyll a content in tea using confocal Raman microscopy

Examples

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Embodiment 1

[0060] The sample stage structure provided by the present invention is as follows image 3 As shown, it includes: a slide glass 2, a cover glass 1 and a stage 3. Both the slide glass 2 and the cover glass 1 are made of quartz.

[0061] The surface of the slide glass 2 is used to place tea samples. The slide glass 2 is rectangular. Two long sides of the slide glass 2 are fixed with a slider 7 respectively. Each slider 7 is fixed with a spring pin and two sliders 7 The position of the spring pin on the corresponding. A silicon chip 5 is embedded on the glass slide 2.

[0062] The cover glass 1 is rectangular, the long side of the cover glass 1 is hinged with one of the sliders 7, and the cover glass 1 is turned over to compress the tea sample. The cover glass 1 is used to keep the surface of the tea leaf flat, and when detecting different points on the tea sample, try to minimize the variation of the object distance to ensure that the Raman detection lens can be accurately positio...

Embodiment 2

[0065] Take 315 Longjing 43 leaves. For each Longjing 43 leaves, randomly cut the leaves on both sides of the veins to obtain two tea samples. The mass of each tea sample is about 0.1g. After the cutting is completed, the actual measurement and records Ww. One part is placed directly in the bag and labeled as Raman spectroscopy material A. The other part is cut into a centrifuge tube and labeled. Add 10mL of 95% alcohol solution and store it in a dark room for about 24h , As ultraviolet spectrophotometric material B. For the same tea leaf, Raman spectroscopy material A and ultraviolet spectrophotometric material B have the same label.

[0066] The Raman spectroscopy material A is fixed on the Raman stage described in Example 1, using a Renishaw confocal laser Raman microscope (Renishaw in Via-Reflex 532 / XYZ), excitation wavelength 532nm; laser intensity 50mW; Integration time 1s; Raman spectrum detection wavelength range is 579.175~3061.95cm -1 ; Resolution 0.2nm; Choose object...

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Abstract

The invention discloses a method of utilizing confocal microscopy for Raman detection of chlorophyll a content in tea leaves. The method includes following steps: 1), putting a tea leaf sample on a sample stage, adopting a Raman spectrometer to acquire Raman spectrum information of the tea leaf sample, and performing full-waveband integral correction; 2), utilizing Raman spectrum intensity at 15 feature wave numbers to build a linear model; 3), collecting correcting spectrum intensity of the tea leaf sample at the 15 feature wave numbers, and utilizing the linear model to predict the chlorophyll a content in the tea leaves to realize nondestructive and quick detection of the chlorophyll a content in the tea leaves. By the method, quick, nondestructive, low-cost and environment-friendly detection of the chlorophyll a content in the tea leaves can be realized quickly and effectively, and complex pretreatment and chemical analysis of the tea leaf sample are not needed, so that operation steps are simplified greatly, detection time is shortened, information of chlorophyll a in the tea leaves is acquired quickly, and detection accuracy is improved.

Description

Technical field [0001] The invention relates to the technical field of chlorophyll a content detection, in particular to a method for detecting the chlorophyll a content in tea by using confocal microscopy Raman. Background technique [0002] The functional shape of plant leaves directly reflects the physiological growth and genetic characteristics of plants, and its changing trend affects the material and energy exchange between the plant and the surrounding environment and the survival strategies formed by the plant adapting to environmental changes. Leaves are the main place for plants to carry out photosynthesis, and photosynthesis is the energy conversion process of light energy into chemical energy, which is the basis for the formation of crop yield and quality. [0003] [Study on the functional traits and photosynthetic characteristics of different leaf positions of tea plants] pointed out that leaves are important organs of plant photosynthesis. Solar energy is first absorb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 李晓丽许凯雯何勇周瑞清张裕莹魏玉震
Owner ZHEJIANG UNIV