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Composite-modified electrode for measuring concentration of 5'-Guanylic acid disodium salt and application thereof

A technology of disodium guanylate and modified electrodes, which is applied to the electrochemical variables of materials, measuring devices, and analytical materials, etc., can solve the problems of poor repeatability, low accuracy, and high detection cost of carbon paste electrodes, and achieve enhanced stability and repeatability, enhanced electrode conductivity, and excellent catalytic performance

Inactive Publication Date: 2017-01-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the traditional detection methods of 5'-GMP mainly include ultraviolet spectrophotometry, capillary electrophoresis, ion exchange chromatography and ion-pair reverse high performance liquid chromatography, etc., but these methods are either not accurate or costly , the steps are cumbersome
Electrochemical methods can also be used to detect 5'-GMP, and are favored by people because of their advantages such as easy operation, good selectivity, and high sensitivity. Most of the research focuses on carbon paste electrodes and glassy carbon electrodes, but carbon paste electrodes always appear Due to the disadvantages of poor repeatability and cumbersome fabrication, the research on glassy carbon electrodes is mostly limited to a single modified material, so it is necessary to prepare a new stable composite modified electrode.

Method used

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  • Composite-modified electrode for measuring concentration of 5'-Guanylic acid disodium salt and application thereof
  • Composite-modified electrode for measuring concentration of 5'-Guanylic acid disodium salt and application thereof
  • Composite-modified electrode for measuring concentration of 5'-Guanylic acid disodium salt and application thereof

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Experimental program
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Effect test

Embodiment 1

[0023] Prepare the gold nano / carboxylated multi-walled carbon nanotube composite modified electrode (Au / PABSA / GCE) for the determination of the concentration of disodium guanylate, comprising the following steps:

[0024] (1) Glassy carbon electrode pretreatment: use α-Al with a particle size of 300nm and 50nm in sequence on the suede 2 o 3 Polish the glassy carbon electrode to the mirror surface with the paste polishing liquid of polishing powder. The electrode was ultrasonically washed in ionized water for 1 min, and then the cleaned glassy carbon electrode was placed in 0.5 mol / L dilute sulfuric acid solution, and cyclic voltammetry activation was performed at a sweep rate of 100 mV / s in the potential range of -0.5 to 1.2 V until the response Finally, rinse the glassy carbon electrode with deionized water and dry it for later use.

[0025] (2) Before modifying the glassy carbon electrode, the electrode needs to be at 1mmol / L K 3 [Fe(CN) 6 ] solution (containing 0.1mol / L...

Embodiment 2

[0038] Prepare the gold nano / carboxylated multi-walled carbon nanotube composite modified electrode (Au / PABSA / GCE) for the determination of the concentration of disodium guanylate, comprising the following steps:

[0039] (1) Glassy carbon electrode pretreatment: use α-Al with a particle size of 300nm and 50nm in sequence on the suede 2 o 3 Polish the glassy carbon electrode to the mirror surface with paste polishing liquid, then apply absolute ethanol and deionized water to ultrasonically wash the electrode for 90s, and then place the cleaned glassy carbon electrode in 0.6mol / L dilute sulfuric acid solution, in- The 0.6-1.3V potential range was activated by cyclic voltammetry at a scan rate of 120mV / s until the response was stable. Finally, the glassy carbon electrode was rinsed with deionized water and dried in the air for later use.

[0040] (2) The pretreated glassy carbon electrode was placed in 1.5mmol / L chloroauric acid (containing 0.05mol / L potassium nitrate) solution...

Embodiment 3

[0044] Prepare the gold nano / carboxylated multi-walled carbon nanotube composite modified electrode (Au / PABSA / GCE) for the determination of the concentration of disodium guanylate, comprising the following steps:

[0045] (1) Glassy carbon electrode pretreatment: use α-Al with a particle size of 300nm and 50nm in sequence on the suede 2 o 3 Polish the glassy carbon electrode to the mirror surface with the paste polishing solution, then use absolute ethanol and deionized water to ultrasonically wash the electrode for 2 minutes, and then place the cleaned glassy carbon electrode in 0.7mol / L dilute sulfuric acid solution, in- The 0.6-1.5V potential range was activated by cyclic voltammetry at a scan rate of 140mV / s until the response was stable. Finally, the glassy carbon electrode was rinsed with deionized water and dried in the air for later use.

[0046] (2) The pretreated glassy carbon electrode was placed in 2mmol / L chloroauric acid (containing 0.15mol / L potassium nitrate) ...

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Abstract

The invention discloses a composite-modified electrode for measuring concentration of 5'-Guanylic acid disodium salt and an application thereof. First, a noble metal nanoparticle modified electrode is prepared through an electrochemical deposition method, and then carbon nanomaterial suspension liquid which is uniformly dispersed in ultrasonic mode is loaded on the surface of the noble metal nanoparticle modified electrode by using a dispensing method so as to prepare the noble metal / nanocomposite modified electrode. The composite-modified electrode for measuring the concentration of the 5'-Guanylic acid disodium salt shows excellent electrocatalytic activity for the 5'-Guanylic acid disodium salt, and has the advantages of being high in sensitivity, good in stability and the like, and simultaneously the preparation process of the electrode is convenient and easy to implement, and the detection process of the electrode is simple and efficient.

Description

technical field [0001] The invention belongs to the technical field of food analysis and detection, and in particular relates to a composite material modified electrode for measuring the concentration of disodium 5'-guanylate and its application. Background technique [0002] Food has a variety of flavors. In addition to the four basic tastes of sweetness, bitterness, saltiness and sourness, there is also a fifth basic taste - umami. Umami is a common flavor in many foods. Umami agents have a good flavor-enhancing effect on vegetables, meat, poultry, milk, aquatic products and even wine. Disodium 5'-guanylate (5'-GMP) is a common umami nucleotide, often used as a food additive, and it has a strong synergistic effect when combined with sodium glutamate. [0003] At present, the traditional detection methods of 5'-GMP mainly include ultraviolet spectrophotometry, capillary electrophoresis, ion exchange chromatography and ion-pair reverse high performance liquid chromatography...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 韦真博张伟林王俊王永维
Owner ZHEJIANG UNIV
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