Tumor blood platelet RNA quantitative detection model and method for tumor early screening

A quantitative detection method and quantitative detection technology, applied in the fields of molecular biology and medicine, can solve the problems of inaccurate diagnosis of tumors, and achieve the effects of early screening of tumors, improvement of survival rate, and good value of tumor diagnosis

Inactive Publication Date: 2017-02-15
上海厚承医学科技有限公司
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Problems solved by technology

In the existing technology, there is no corresponding mature technology to use tumor-associated platelets for more accurate diagnosis of tumors

Method used

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  • Tumor blood platelet RNA quantitative detection model and method for tumor early screening
  • Tumor blood platelet RNA quantitative detection model and method for tumor early screening
  • Tumor blood platelet RNA quantitative detection model and method for tumor early screening

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Embodiment

[0079] The above-mentioned tumor platelet RNA detection method for tumor diagnosis was used to detect the expression of target tumor platelet RNA in the plasma of 50 tumor patients and 20 healthy people (normal controls), namely CD79A (ENSG00000105369), CD81 (ENSG00000110651), SYTL1 (ENSG00000142765 ), CENPC(ENSG00000145241), TTN(ENSG00000155657), RHOH(ENSG00000168421), ZNF101(ENSG00000181896), TRABD2A(ENSG00000186854), TRAC(ENSG000002291624) △Ct value).

[0080] Concrete detection method comprises the following steps:

[0081] 1. Collection and storage of tumor platelet RNA

[0082] Collect 4ml of venous blood from the subject into an EDTA anticoagulant tube, gently invert it several times and let it stand for 10 minutes (centrifuge the plasma within 1 hour at room temperature), centrifuge at 120g at room temperature for 20 minutes, divide into two layers, and absorb the upper layer of plasma (transparent Pale yellow liquid, high blood lipid samples are milky samples), tran...

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Abstract

The invention discloses a tumor blood platelet RNA quantitative detection model for tumor early screening. The model comprises PCR (polymerase chain reaction) detection specific primers. The PCR detection specific primers include an F-end primer and an RT primer which are shown as SEQ ID NO.1, an F-end primer and a RT primer shown as SEQ ID NO.2, an F-end primer and an RT primer which are shown as SEQ ID NO.3, an F-end primer and an RT primer which are shown as SEQ ID NO.4, an F-end primer and an RT primer which are shown as SEQ ID NO.5, an F-end primer and an RT primer which are shown as SEQ ID NO.6, an F-end primer and an RT primer which are shown as SEQ ID NO.7, an F-end primer and an RT primer which are shown as SEQ ID NO.8 and an F-end primer and an RT primer which are shown as SEQ ID NO.9. The invention further discloses a tumor blood platelet RNA quantitative detection method for tumor early screening. The tumor blood platelet RNA quantitative detection model and method has the advantages that tumor early screening is realized, tumor pathological identification and clinical diagnosis are assisted, and survival rate of patients is increased.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, in particular to a tumor platelet RNA quantitative detection model and method for early screening of tumors. Background technique [0002] With the increasing incidence and mortality of cancer, cancer is not only the main cause of death in China, but also a major public health problem that seriously threatens human health and restricts economic and social development. Early diagnosis of tumors means early intervention and treatment, which is of great significance to the prognosis of patients. At present, the diagnosis of tumors mainly relies on traditional tumor tissue clinical symptoms, radiological imaging, biochemical detection, and pathology, and most of the methods are invasive, causing pain to patients, and affecting the implementation of this technology. Physicians have high demands. The non-invasive examination methods for tumors are mainly serum tumor markers, such as AFP...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q1/6851C12Q2600/158C12Q2545/113
Inventor 卞胜超
Owner 上海厚承医学科技有限公司
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