Tumor blood platelet RNA quantitative detection model and method for tumor early screening
A quantitative detection method and quantitative detection technology, applied in the fields of molecular biology and medicine, can solve the problems of inaccurate diagnosis of tumors, and achieve the effects of early screening of tumors, improvement of survival rate, and good value of tumor diagnosis
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[0079] The above-mentioned tumor platelet RNA detection method for tumor diagnosis was used to detect the expression of target tumor platelet RNA in the plasma of 50 tumor patients and 20 healthy people (normal controls), namely CD79A (ENSG00000105369), CD81 (ENSG00000110651), SYTL1 (ENSG00000142765 ), CENPC(ENSG00000145241), TTN(ENSG00000155657), RHOH(ENSG00000168421), ZNF101(ENSG00000181896), TRABD2A(ENSG00000186854), TRAC(ENSG000002291624) △Ct value).
[0080] Concrete detection method comprises the following steps:
[0081] 1. Collection and storage of tumor platelet RNA
[0082] Collect 4ml of venous blood from the subject into an EDTA anticoagulant tube, gently invert it several times and let it stand for 10 minutes (centrifuge the plasma within 1 hour at room temperature), centrifuge at 120g at room temperature for 20 minutes, divide into two layers, and absorb the upper layer of plasma (transparent Pale yellow liquid, high blood lipid samples are milky samples), tran...
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