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A method for tissue culture and rapid propagation of Primula huaiji

A technology of primula moss and tissue culture, which is applied in the fields of botany equipment and methods, horticultural methods, plant regeneration, etc., and can solve the problems of tissue culture and seedling propagation of primula moss in Huaiji, slow cutting propagation speed, Low seed germination rate and other problems, to achieve the effect of resource protection and sustainable utilization, simple and affordable operation, and good growth

Active Publication Date: 2018-09-28
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Huaiji Primula is not fruitful under natural conditions, and requires insects or artificial pollination, and the seed germination rate is low; while the cutting propagation speed is slow and the reproduction coefficient is low, which will also destroy extremely precious wild resources
[0004] At present, there are no reports on the tissue culture and seedling propagation of Primula huaiji at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Disinfection of explants and induction culture of the first generation: Cut the thick young leaves of Primula huaiji as explants during the growing season, rinse with detergent for 20 minutes, rinse with running water for 30 minutes, and then place them on the ultra-clean workbench. Soak in 75% ethanol solution by volume fraction for 30s, mass fraction 0.1% HgCl 2 Disinfect the solution for 4 minutes, rinse with sterile water for 5 times, dry the surface water with sterile filter paper, cut the leaves into small pieces of 1.0cm×2.0cm, inoculate them on the primary induction medium, and cultivate them at a temperature of 25±2°C. The light intensity is 1500lx, and the light time is 12h / d. After culturing for about 30 days, calluses began to grow around the cut pieces and a few buds germinated. After continuing to cultivate for about 38 days, many young shoots germinated on the callus. The success rate of disinfection is 85%. The primary induction medium is as follow...

Embodiment 2

[0027] 1. Disinfection of explants and induction culture of the first generation: Cut the thick young leaves of Primula huaiji as explants during the growing season, rinse with detergent for 20 minutes, rinse with running water for 30 minutes, and then place them on the ultra-clean workbench. Soak in 75% ethanol solution by volume fraction for 30s, mass fraction 0.1% HgCl 2 Disinfect the solution for 5 minutes, rinse 6 times with sterile water, dry the surface water with sterile filter paper, cut the leaves into small pieces of 0.5cm×1.0cm, inoculate them on the primary induction medium, and cultivate them at a temperature of 25±2°C. The light intensity is 1500lx, and the light time is 12h / d. Around the 19th day of culture, the callus began to grow around the cutting and a little bud germinated. A large number of young shoots germinated on the callus after continuing to cultivate for about 27 days. The success rate of disinfection is 85%. The primary induction medium is as ...

Embodiment 3

[0032] 1. Disinfection of explants and induction culture of the first generation: Cut the thick young leaves of Primula huaiji as explants during the growing season, rinse with detergent for 20 minutes, rinse with running water for 30 minutes, and then place them on the ultra-clean workbench. Soak in 75% ethanol solution by volume fraction for 30s, mass fraction 0.1% HgCl 2 Disinfect the solution for 4 minutes, rinse with sterile water for 5 times, dry the surface water with sterile filter paper, cut the leaves into small pieces of 1.0cm×2.0cm, inoculate them on the primary induction medium, and cultivate them at a temperature of 25±2°C. The light intensity is 1500lx, and the light time is 12h / d. After 22 days or so of cultivation, calluses began to grow around the cut pieces and a few buds germinated. Continue to cultivate for about 30 days and germinate many tiny young shoots on the callus. The success rate of disinfection is 85%. The primary induction medium: each liter ...

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Abstract

The invention provides huaiji primulina tissue culture and a rapid propagation method. Fleshy and tender leaves of huaiji primulina are adopted as an explant; in an adventitious bud induction stage, a cluster bud propagation stage, a seedling-strengthening and rooting stage, a transplanting stage and other stages, a suitable culture medium formula and suitable culture conditions suitable for cluster bud induction, propagation, rooting, transplantation and the like of the huaiji primulina in each corresponding culture stage are selected so as to achieve the tissue culture and rapid propagation of huaiji primulina seed seedlings; a large number of high-quality seed seedlings can be obtained by culture and propagation in a relatively short period of time, and the seed seedlings have the characteristics of being high in genetic stability, fast in seedling emergence, strong, good in seed seedling quality, strong in resistance and good in growth; therefore, the high-quality seed seedlings are provided to meet the requirements of an ex-situ conservation and seed introduction research of the huaiji primulina species and resource exploitation and utilization, and resource protection and sustainable utilization of the huaiji primulina are thus achieved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and rapid propagation, in particular to a method for tissue culture and rapid propagation of Primula huaiji. Background technique [0002] Primulina huaijiensis (Primulina huaijiensis Z.L.Ning&J.Wang) is a perennial herb of the Gesneriaceae family Primulus genus. It was discovered in an isolated karst small limestone cave in Huaiji County, Guangdong Province in 2010. It was officially released in 2013. Named as Huaiji Primula lettuce published. There is only one wild population of Primula Huaiji, about 200 plants, located near a village in Huaiji County. It is a rare and endangered plant because of its small number and its habitat is easily damaged by human disturbance. In addition, its corolla lip has two membranous appendages, which is the only species in Gesneriaceae with this morphological feature. This species is of great significance for the research on the taxonomy of Primula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李冬梅胡彬宁祖林陈玲康明廖景平
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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