A kind of anti-porcine epidemic diarrhea virus s protein hybridoma cell line and its application

A technology of hybridoma cell line and porcine epidemic diarrhea, which is applied in the direction of antiviral immunoglobulin, antiviral agent, antibody, etc., and can solve the problem of time-consuming

Active Publication Date: 2020-08-04
GUANGDONG WENS DAHUANONG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ELISA is one of the most widely used detection methods at present. It is simple and easy to operate but takes a long time. As a new simple, fast, accurate and intuitive detection method, colloidal gold immunochromatography is becoming well known to everyone. Combined with monoclonal antibody technology, it has good specificity and sensitivity

Method used

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  • A kind of anti-porcine epidemic diarrhea virus s protein hybridoma cell line and its application
  • A kind of anti-porcine epidemic diarrhea virus s protein hybridoma cell line and its application
  • A kind of anti-porcine epidemic diarrhea virus s protein hybridoma cell line and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 A hybridoma cell line resistant to porcine epidemic diarrhea virus S protein

[0040] A hybridoma cell line resistant to porcine epidemic diarrhea virus S protein, named hybridoma cell line 7D3F11, preserved in the China Center for Type Culture Collection, the preservation address is Wuhan, Wuhan University, China, and the preservation number is CCTCC NO: C201660. Date: April 21, 2016.

Embodiment 2

[0041] Example 2 Monoclonal antibody secreted by hybridoma cell line 7D3F11

[0042] The monoclonal antibody is prepared by the following method:

[0043] (1) Immune mice

[0044] Take 6-8 weeks old BALB / c mice for intraperitoneal immunization with purified PEDV S protein plus an equal amount of Freund's complete adjuvant, first immunize each mouse with 50ug; 2 weeks later emulsify with Freund's incomplete adjuvant, the dose As before, the immunization was boosted twice by subcutaneous multi-point injection; 2 weeks later, intraperitoneal injection, the dose was the same as before; from 3 weeks after immunization, blood was collected from the tail of the mouse to measure the antibody titer (ELISA), and the ELISA effect was selected 10 days after the fourth immunization. The mouse with the highest valence was prepared for fusion, and the immunization was boosted once 3 days before fusion;

[0045] (2) ELISA method establishment

[0046] Using the purified PEDV S protein as...

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Abstract

The invention relates to a hybridoma cell line for resisting porcine epidemic diarrhea virus S proteins. The hybridoma cell line is named a hybridoma cell line 7D3F11 and is preserved in the China Center for Type Culture Collection, wherein the preservation address is at Wuhan University in Wuhan, the People's Republic of China, and the preservation number is CCTCC No. C201660. The hybridoma cell line 7D3F11 for resisting the porcine epidemic diarrhea virus S proteins, prepared by adopting a biotechnology means, establishes a basis for detection of PEDV infection.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a hybridoma cell strain resistant to porcine epidemic diarrhea virus S protein and application thereof. Background technique [0002] Porcine epidemic diarrhea (Porcine epidemic diarrhea, PED) was first reported in the UK, followed by Belgium, Germany, Canada, Japan, Switzerland and other countries. The disease was discovered in my country in 1976 and reported successively. Porcine epidemic diarrhea (PED) is caused by porcine epidemic diarrhea virus (PEDV), and is a highly contagious disease characterized by pig vomiting and severe diarrhea and dehydration as the main clinical symptoms. Pigs of different ages and different breeds are susceptible, and the incidence of suckling piglets, weaned piglets and fattening pigs can reach 100%, and the mortality rate is as high as 95%. [0003] PEDV belongs to the genus Coronaviridae of the family Coronaviridae of the order Nid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/10A61K39/42A61P31/14
CPCA61K39/00C07K16/10
Inventor 陈瑞爱王亚欣赵大伟唐满华
Owner GUANGDONG WENS DAHUANONG BIOTECH
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