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Far-field super-resolution imaging system assisted by hollow dielectric microspheres

A super-resolution imaging and medium technology, applied in the field of imaging systems, can solve the problems of resolution limit limitation, etc., and achieve the effects of small focal spot, high imaging resolution, superior focusing and imaging characteristics

Active Publication Date: 2019-01-11
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to its own structural characteristics, no matter how its size and material change, its resolution limit is also limited.

Method used

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  • Far-field super-resolution imaging system assisted by hollow dielectric microspheres
  • Far-field super-resolution imaging system assisted by hollow dielectric microspheres
  • Far-field super-resolution imaging system assisted by hollow dielectric microspheres

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Embodiment Construction

[0016] The far-field super-resolution imaging system assisted by hollow dielectric microspheres uses the central hollow part to modulate the converging light beam, so as to obtain better focusing effect and imaging resolution. The dielectric microsphere lens can increase the numerical aperture of the microscopic objective lens, so as to obtain higher resolution than the ordinary microscopic objective lens. The hollow dielectric microsphere lens is equivalent to the centrally blocked dielectric microsphere lens, which can filter out the low-frequency components in the light and increase the ratio of high-frequency components, so that a smaller focal spot and higher imaging resolution can be obtained.

[0017] Such as figure 1 Schematic diagram of the structure of the hollow dielectric microsphere lens shown. The central part of the lens is a sphere with a radius of r , the filling material is air, and its refractive index is n 0 , the outer layer is a spherical shell with a...

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Abstract

The invention relates to a hollow microsphere for far-field auxiliary super resolution imaging system which the hollow micro-sphere is positioned on the surface of detecting samples, then dispose the sample in the focal plane of microscope objective, when micro-nano structure of the surface on the sample passes through the co-axial optical path which is sequentially formed by the hollow microsphere-lens, microscope objective and tube lens. The imaging detector acquires the images of the test sample, and reflects an imaging resolution which is exceeded the diffraction limitation. Compared with a conventional microsphere, hollow microsphere-lens is characterized by superior focus capacity, better imaging performance and smaller focal spots under the same condition of light and microscope objective. The hollow micro-sphere-lens provides with higher quality imaging resolution when the system is imaging.

Description

technical field [0001] The invention relates to an imaging system, in particular to a far-field super-resolution imaging system assisted by hollow medium microspheres. Background technique [0002] Due to the limitation of the diffraction limit of ordinary optical microscopes, its imaging resolution depends on the incident wavelength and the numerical aperture of the microscope objective lens, which is usually not less than half of the incident light wavelength, that is, 200 nanometers. When the characteristic size of the imaging target is less than 200 nanometers, such as some organelles and viruses in biological samples, the optical microscope is helpless. In order to break through the diffraction limit and obtain clear images of nanoscale structures, people use fluorescence microscopy to dye the samples to be observed, and then use the photosensitive switch characteristics of dye fluorescent molecules to obtain the fine structure of biological samples. However, this meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B27/58G02B21/36
CPCG02B21/361G02B27/58
Inventor 凌进中严锦雯贾星伟张跃华隋国荣张大伟庄松林
Owner UNIV OF SHANGHAI FOR SCI & TECH