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Sample separation and transfer device and sample analysis method

A technology of sample and transfer film, which is used in measurement devices, analysis materials, material analysis by electromagnetic means, etc., to achieve high-efficiency effects of immunoblotting experiments

Inactive Publication Date: 2017-02-22
SHARP LIFE SCI CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, the prior art as described above has a problem that separation, transfer, and subsequent processing of the specimen cannot be performed automatically.

Method used

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  • Sample separation and transfer device and sample analysis method
  • Sample separation and transfer device and sample analysis method
  • Sample separation and transfer device and sample analysis method

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Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0027] When one embodiment (Embodiment 1) of the present invention is described based on the drawings, it will be as follows.

[0028] First, refer to Figure 1 ~ Figure 4 A schematic configuration of the sample separation and transfer device 100 according to Embodiment 1 will be described. figure 1 It is a perspective view schematically showing the configuration of the sample separation and transfer device 100 . figure 2 is a cross-sectional view schematically showing the configuration of the sample separation and transfer device 100 . image 3 It is a perspective view showing the positions of the nozzles in the sample separation and transfer device 100 . Figure 4 It is a schematic diagram for explaining the function of the spacer in the sample separation and transfer device 100 .

[0029] Such as figure 1 , figure 2 As shown, the sample separation transfer device 100 separates the test object by electrophoresis, discharges the separated test object from the discharge...

Embodiment approach 2

[0106] if based on Figure 5 Another embodiment (Embodiment 2) of the present invention will be described as follows. In addition, for convenience of description, components having the same functions as those described in Embodiment 1 are denoted by the same reference numerals, and description thereof will be omitted.

[0107] The difference between the second embodiment and the first embodiment is the shape of the nozzles 14 a and 14 b and the configuration of the bottom surface of the anode buffer tank 30 . Other points are the same as those in Embodiment 1.

[0108] (nozzle)

[0109] In Embodiment 2, such as Figure 5 As shown, for the nozzles 14a, 14b, the ends with the openings 15a, 15b are bent into an L along the side and bottom surface in the anode buffer tank 30, and the openings 15a, 15b are opposite to the bottom surface of the anode buffer tank 30 Open in the vertical direction.

[0110] Thereby, collection|recovery of the waste liquid (used liquid) in the ano...

Embodiment approach 3

[0115] if based on Figure 6 Another embodiment (Embodiment 3) of the present invention will be described as follows. In addition, for convenience of description, components having the same functions as those described in Embodiment 1 are denoted by the same reference numerals, and description thereof will be omitted.

[0116] The difference between Embodiment 3 and Embodiment 1 is the material and structure of the bottom surface of the anode buffer tank 30 . Other points are the same as those in Embodiment 1.

[0117] (bottom surface of anode buffer tank)

[0118] In Embodiment 3, such as Figure 6 As shown, a hydrophilic region (partial region) 71 and a hydrophobic region (other region) 72 exist on the bottom surface of the anode buffer tank 30 . More specifically, the hydrophilic region 71 exists in the second region 36 . That is, the bottom surface of the anode buffer tank 30 (specifically, the second region 36 ) includes a hydrophilic region 71 and a hydrophobic regi...

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Abstract

Provided is a novel technology for automatically separating and transferring a specimen and carrying out subsequent processing. The present invention is a sample separation and transfer device (100) that separates a specimen through electrophoresis, discharges the separated specimen from a discharge part (50a) inside a buffer tank (30), and transfers the separated specimen to a transfer film (1) by bringing the transfer film (1) into contact with the discharge part (50a) and moving the transfer film (1), wherein the device is provided with liquid feed pumps (11a, 11b) for exchanging the liquid filling the buffer tank (30).

Description

technical field [0001] The present invention relates to electrophoresis technology, in particular, to a sample separation transfer device for separating a subject by electrophoresis, transferring the separated subject to a transfer membrane, and performing subsequent processing. Background technique [0002] After the completion of the Human Genome Project, until now, the relationship between various diseases and biological macromolecules has gradually become clear. In particular, proteins, which are one of biological macromolecules, are directly related to the functions of cells, organs, and viscera of organisms, and there are many problems caused by differences in amino acid arrangement and three-dimensional structure, chemical modifications such as sugar chains, and phosphorylation. The possibility of disease in this situation is starting to become clear. [0003] In such a situation, many proteome analyzes are performed. The proteome refers to the entirety of proteins ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/44708G01N27/44739G01N2035/0487G01N27/4473G01N1/30G01N1/31G01N27/44747
Inventor 后藤真一木下英树
Owner SHARP LIFE SCI CORP