Repair method of nitrogen-fixing Anabaena cells damaged by als inhibitor herbicides

A remediation method and technology of Anabaena, applied in the biological field, can solve the problems of nitrogen-fixing Anabaena lethality, soil barrenness, extinction, etc., and achieve the effects of maintaining ecological balance, protecting ecological resources, and promoting synthesis.

Inactive Publication Date: 2019-12-10
SHANGHAI JIAO TONG UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The role of nitrogen-fixing Anabaena in farmland is obvious. However, nitrogen-fixing Anabaena has a photosynthetic system and target ALS enzyme similar to higher plants. ALS inhibitor herbicides can control the nitrogen-fixing fish in farmland while controlling weeds. Algae produce lethal damage, making it face the danger of extinction, which is also one of the main factors that make the soil barren
But so far, there is no report on the method of repairing the damage of Anabaena nitrogen-fixing algae by ALS inhibitor herbicides in the world. As a result, under the extensive use of ALS inhibitor herbicides, Nitrogen-fixing Anabaena passively bears a large amount of damage. casualties, even death

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Repair method of nitrogen-fixing Anabaena cells damaged by als inhibitor herbicides
  • Repair method of nitrogen-fixing Anabaena cells damaged by als inhibitor herbicides
  • Repair method of nitrogen-fixing Anabaena cells damaged by als inhibitor herbicides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Implementation period: April 2016 to May 2016

[0030] Test plan: There are 8 treatments in this test, the blank control CK treatment group (without adding bensulfuron-methyl and restorative agent), the drug-added control treatment group (adding 0.1mg / L bensulfuron-methyl, without adding restorative agent), other On the 3rd day after adding 0.1mg / L bensulfuron-methyl to the 6 treatment groups, MgSO was added at a volume ratio of 1:100 4 Concentration of 10, 20, 30, 40, 50, 60g / L repair agent, other ingredients are the same, KNO 3 The concentration is 20g / L, the molybdic acid is 0.05g / L, and the sucrose is 15g / L. Each treatment was repeated 3 times.

[0031] 1) When 0.1 mg / L bensulfuron-methyl was added to treat the nitrogen-fixing Anabaena cell culture medium on the 3rd day, the 6 treatment groups were added MgSO at a volume ratio of 1:100 4 Concentration of 10, 20, 30, 40, 50, 60g / L repair agent, other ingredients are the same, KNO 3 The concentration is 20g / L, the...

Embodiment 2

[0036] Implementation time: May 2016 to July 2016

[0037] Test plan: There are 8 treatments in this test, the blank control CK treatment group (without adding bensulfuron-methyl and restorative agent), the drug-added control treatment group (adding 0.1mg / L bensulfuron-methyl, without adding restorative agent), other On the 3rd day after adding 0.1mg / L bensulfuron-methyl to the 6 treatment groups, KNO was added at a volume ratio of 1:100 3 Concentration of 10, 20, 30, 40, 50, 60g / L repair agent, other ingredients are the same, MgSO 4 The concentration is 30g / L, the molybdic acid is 0.05g / L, and the sucrose is 15g / L. Each treatment was repeated 3 times.

[0038] 1) When 0.1mg / L bensulfuron-methyl was added to treat the nitrogen-fixing Anabaena cell culture medium on the 3rd day, the 6 treatment groups were added KNO at a volume ratio of 1:100 3 Concentration of 10, 20, 30, 40, 50, 60g / L repair agent, other ingredients are the same, MgSO 4 The concentration is 30g / L, the mol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a repair method for Anabaena azotica cells damaged by ALS inhibitor-based weeding agents. The repair method comprises that a repair agent comprising MgSO4, KNO3, molybdic acid and sucrose is prepared; and within the use of the ALS inhibitor-based weeding agent for 1-3 days, the repair agent is added to the damaged Anabaena azotica cell fluid according to a volume ratio of 1:100, and the culture is continuously performed for 7 days so as to promote the synthesis of the Anabaena azotica cell photosynthetic pigments, improve the activity of the anti-oxidation enzyme system, and restore the growing vitality. According to the present invention, by reducing the adverse effect of the ALS inhibitor-based weeding agent on the Anabaena azotica, the damaged Anabaena azotica cells can be effectively repaired, the synthesis of the Anabaena azotica cell photosynthetic pigments can be promoted, the activity of the anti-oxidation enzyme system can be improved, the growing vitality can be restored, the effects of soil structure improving, pesticide consumption reducing, ecological balance maintaining and the like can be provided, and the purposes of ecological resource protection and sustainable management can be achieved.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for repairing nitrogen-fixing anabaena cells damaged by ALS inhibitor herbicides. Background technique [0002] In recent years, with the extensive use of agrochemicals such as herbicides and nitrogen fertilizers, while bringing efficient agricultural production, it has also caused great harm to the agricultural ecological environment. Agricultural non-point source pollution has become an urgent problem to be solved worldwide. Surface water pollution and eutrophication caused by nitrogen-containing fertilizers and herbicides will have a large number of negative impacts on the human body and the ecological environment. In addition, the extensive use of herbicides and nitrogen fertilizers has seriously compacted the soil, which not only affects the yield of crops, but also affects important livelihood issues such as the country and the people's security. my country's 1.8 billion mu of ara...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38
Inventor 沈健英廖谨之郭利利
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap