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Method for simultaneously detecting trichlorfon and moncrotophos through molecularly imprinted solid phase extraction and liquid chromatography

A technology of molecular imprinting and liquid chromatography, which is applied in the simultaneous separation and detection of trichlorfon and monocrotophos, and the rapid and sensitive detection of trichlorfon and monocrotophos, can solve the problems of specific adsorption performance, selective adsorption performance and low performance, and achieve high stability performance, improved adsorption performance, and long service life

Active Publication Date: 2017-03-08
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects that the traditional single polymer can only have specific adsorption performance and low selective adsorption performance for one kind of pesticide, synthesize the molecularly imprinted polymer with mixed template and improve the selective adsorption performance of the polymer, and provide A new method for simultaneous detection of trichlorfon and monocrotophos

Method used

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  • Method for simultaneously detecting trichlorfon and moncrotophos through molecularly imprinted solid phase extraction and liquid chromatography

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Embodiment 1

[0024] 1. Preparation of Molecularly Imprinted Polymer MIL@MIP with High Adsorption Performance

[0025] Add 3mL chloroform solution, 0.5mmol trichlorfon, 0.75mmol monocrotophos and 4mmol methacrylic acid (MAA) into a 100mL stoppered round bottom flask and stir for 30min; then add 10.0mg MIL-101 to fully react for 60min, then add 10mmol ethylene glycol dimethacrylate (EDMA) and 50mg azobisisobutyronitrile (AIBN) initiated thermal polymerization for 24h. A mixed solution of 120mL methanol and 40mL glacial acetic acid was used as the extraction solvent, and the molecularly imprinted polymer MIL@MIP was obtained after continuous elution for 48h.

[0026] 2. Molecularly imprinted solid phase extraction-high performance liquid chromatography process

[0027] Accurately weigh 200 mg of the molecularly imprinted polymer MIL@MIP prepared in step 1) and load it into an empty solid-phase extraction column. Rinse and activate the packed extraction column with methanol and ultrapure wat...

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Abstract

The invention relates to a method for simultaneously detecting trichlorfon and moncrotophos through molecularly imprinted solid phase extraction and liquid chromatography. A high-adsorption-performance molecularly imprinted polymer MIL@MIP is prepared; a hollow molecularly imprinted solid phase extraction column is filled with the high-adsorption-performance molecularly imprinted polymer MIL@MIP, and liquid chromatogram is introduced for analysis; the concentration of a trichlorfon solution and the concentration of a moncrotophos solution serve as the horizontal coordinates respectively, the area of a chromatographic peak serves as the longitudinal coordinate, and a trichlorfon standard curve and a moncrotophos standard curve are drawn respectively; the content of trichlorfon and the content of moncrotophos in an analyte are calculated according to the drawn standard curves. A novel metal framework material MIL-101 with porosity and a large specific surface area serves as a support carrier, and adsorption performance of the prepared polymer is greatly improved; besides, the metal framework material has high selectivity to both trichlorfon and moncrotophos and can take the place of a single polymer to be used in solid-phase extraction; the prepared molecularly imprinted polymer prepared through the method can be repeatedly used for about 20 times, and cost is greatly reduced.

Description

technical field [0001] The invention relates to a method for simultaneous separation and detection of trichlorfon and monocrotophos based on molecular imprinted solid-phase extraction technology, which belongs to the technical field of food safety detection, in particular to a rapid and sensitive detection method for trichlorfon and monocrotophos. Background technique [0002] Trichlorphon (trichlorphon, O, O-dimethyl-(2,2,2-trichloro-1-hydroxyethyl) phosphate) is an organophosphorus spectrum insecticide, Monocrotophos (O , O-dimethyl-2-methylcarbamoyl-1-methylvinyl phosphate) is an efficient systemic organophosphate insecticide. [0003] Both of these two pesticides have the characteristics of high efficiency, low toxicity, low residue, and good water solubility, so they are widely used in agriculture, forestry, horticulture, animal husbandry, and hygiene. Also because of its wide application and increasingly serious abuse, its damage to animal nervous system, carcinogenic...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 徐志祥张昕孔非凡李德青
Owner SHANDONG AGRICULTURAL UNIVERSITY
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