Method for simultaneously detecting trichlorfon and moncrotophos through molecularly imprinted solid phase extraction and liquid chromatography

Abstract

The invention relates to a method for simultaneously detecting trichlorfon and moncrotophos through molecularly imprinted solid phase extraction and liquid chromatography. A high-adsorption-performance molecularly imprinted polymer MIL@MIP is prepared; a hollow molecularly imprinted solid phase extraction column is filled with the high-adsorption-performance molecularly imprinted polymer MIL@MIP, and liquid chromatogram is introduced for analysis; the concentration of a trichlorfon solution and the concentration of a moncrotophos solution serve as the horizontal coordinates respectively, the area of a chromatographic peak serves as the longitudinal coordinate, and a trichlorfon standard curve and a moncrotophos standard curve are drawn respectively; the content of trichlorfon and the content of moncrotophos in an analyte are calculated according to the drawn standard curves. A novel metal framework material MIL-101 with porosity and a large specific surface area serves as a support carrier, and adsorption performance of the prepared polymer is greatly improved; besides, the metal framework material has high selectivity to both trichlorfon and moncrotophos and can take the place of a single polymer to be used in solid-phase extraction; the prepared molecularly imprinted polymer prepared through the method can be repeatedly used for about 20 times, and cost is greatly reduced.

Description

technical field [0001] The invention relates to a method for simultaneous separation and detection of trichlorfon and monocrotophos based on molecular imprinted solid-phase extraction technology, which belongs to the technical field of food safety detection, in particular to a rapid and sensitive detection method for trichlorfon and monocrotophos. Background technique [0002] Trichlorphon (trichlorphon, O, O-dimethyl-(2,2,2-trichloro-1-hydroxyethyl) phosphate) is an organophosphorus spectrum insecticide, Monocrotophos (O , O-dimethyl-2-methylcarbamoyl-1-methylvinyl phosphate) is an efficient systemic organophosphate insecticide. [0003] Both of these two pesticides have the characteristics of high efficiency, low toxicity, low residue, and good water solubility, so they are widely used in agriculture, forestry, horticulture, animal husbandry, and hygiene. Also because of its wide application and increasingly serious abuse, its damage to animal nervous system, carcinogenic...

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects that the traditional single polymer can only have specific adsorption performance and low selective adsorption performance for one kind of pesticide, synthesize the molecularly imprinted polymer with mixed template and improve the selective adsorption performance of the polymer, and provide A new method for simultaneous detection of trichlorfon and monocrotophos

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