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Creatinine picric acid method detection kit being strong in anti-interference capability

A detection kit and picric acid technology, applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of long analysis time, high analysis cost and poor repeatability of test results. and other problems to achieve the effect of improving stability and reducing positive interference

Active Publication Date: 2017-04-05
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages: A small amount of residual protein will be adsorbed on the inner wall of the capillary, resulting in poor repeatability of the test results
Disadvantages: high analysis cost, expensive liquid chromatograph price and daily maintenance cost, long analysis time

Method used

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  • Creatinine picric acid method detection kit being strong in anti-interference capability
  • Creatinine picric acid method detection kit being strong in anti-interference capability
  • Creatinine picric acid method detection kit being strong in anti-interference capability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A detection kit for creatinine picric acid method with strong anti-interference ability, including reagent R1 and reagent R2:

[0040] 1) The composition of its R1 is:

[0041] Disodium phosphate 78mmol / L

[0042] Sodium carbonate 0.1mmol / L

[0043] 2,4-Dinitrophenylhydrazine 5mmol / L

[0044] Sodium azide 0.5 g / L

[0045] Potassium ferricyanide 100 μl / L

[0046] Sodium hypochlorite 4mmol / L

[0047] Diethylamine 0.05%

[0048] Sodium hydroxide 0.2mol / L

[0049] 2) The components of reagent R2 are:

[0050] Picric acid 17.2mmol / L

[0051] Triton-100 2ml / L.

[0052] 3) The method of using the reagents in this example:

[0053] The creatinine detection reagent described in this example is used in an automatic biochemical analyzer with dual reagent functions, such as Hitachi 7180 automatic analyzer, etc., and is determined by the endpoint method. Place R1 and R2 on the corresponding reagent positions according to the ratio of 3:1, and place distilled water, standa...

Embodiment 2

[0058] Interference test: take fresh mixed serum, divide it into 2 equal parts, then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then, the reagents obtained in Example 1 were used to compare with the common and recognized uric acid (UA) reagents in the market to measure the content of UA in serum. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0059] surface 2 Example reagent anti-interference performance comparison

[0060]

[0061] As can be seen from Table 2, the reagent of Example 1 has a concentration of ascorbic acid ≤ 1704 μmol / L, α-ketoglutarate 20 mmol / L, bilirubin ≤ 684 μmol / L, hemoglobin ≤ 10 g / L, triglyceride ≤ 22.6 mmol / L There was no significant interference with the test results. However, the reagents of the control group were ...

Embodiment 3

[0063] correlation experiment

[0064] Using the formula of Example 1 to prepare reagents, and compared with the common creatinine kit approved by the State Food and Drug Administration in the market, 20 clinical serum samples were tested at the same time, and the test results are shown in Table 3. And obtained the correlation curve of the two reagents (such as figure 1As shown), the test results show that the correlation coefficient of the two kits is 0.999, indicating that there is a great correlation between the two.

[0065] surface 3 Example 1 The test results are compared with the common and recognized creatinine assay kits in the market

[0066]

[0067] The calibrators and quality controls used in the test were:

[0068] Calibrator: Landox composite standard (926UN), in which the content of CREA is 133 μmol / L.

[0069] Quality control product: Landau composite quality control (952UN), in which the target value of CREA is 121 μmol / L, and the target value r...

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Abstract

The invention provides a creatinine picric acid method detection kit being strong in anti-interference capability and relates to the technical field of creatinine detection (picric acid method), and especially realtes to a creatinine detection reagent. A reagent R1 includes disodium phosphate, sodium carbonate, 2,4-dinitrophenylhydrazine, potassium ferricyanide, sodium hypochlorite and sodium hydroxide, and sodium azide and diethylamine as preservatives. The reagent R1 contains the potassium ferricyanide and 2,4-dinitrophenylhydrazine, so that the anti-interference capability of the reagent is significantly improved. A reagent R2 includes the picric acid and triton-100.

Description

technical field [0001] The invention relates to the technical field of creatinine detection, in particular to a creatinine detection kit with strong anti-interference ability. Background technique [0002] Creatinine is formed by the irreversible conversion of phosphocreatine in muscle. Serum creatinine ( CREA ) is a commonly used test item in clinical practice, and it is also one of the emergency renal function test items. Serum creatinine is an effective indicator for evaluating glomerular filtration rate (GFR) and diagnosing acute and chronic renal failure. Creatinine is easily soluble in water (80.1 g / L, 16 ℃). Under normal circumstances, the content of creatinine in the human body is basically stable, generally maintained at 3-14 mg / L. When the creatinine content is higher than the normal range, it is filtered through the glomerulus and excreted with the urine without being reabsorbed by the glomerulus. When the kidney function is damaged, the normal excretion of cr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 谭柏清李家朋甘宜梧王春艳王绮
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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