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Method for producing lycorine and lycoramine from lycoris bulb calluses

A callus, lycoris bulb technology, applied in the directions of horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as a large market gap of lycoris plants, achieve rich raw materials, small occupied space, alleviation of endangered and severe insufficient effect

Inactive Publication Date: 2017-04-19
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem that there is a relatively large gap in the market of lycoris plants for extracting raw materials of lycorine and lycoris, the purpose of the present invention is to provide a method for producing lycorine and lycolicin through Chinese lycoris bulb callus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of method utilizing Chinese lycoris bulb callus to produce lycorine and ricolamin comprises the following steps:

[0037] A) Selection and sterilization of explants: take the bulbs of 'Chinese Lycoris' as explants, soak and rinse with 2% to 3% detergent solution, and shake at 100-150 rpm for 15-20 minutes , then rinsed with clean water, disinfected with 75% alcohol, 0.15% mercuric chloride sterilized, soaked for 10 minutes, rinsed with sterile water 8 times, and set aside;

[0038] B. Inoculation of explants: Inoculate the explants prepared in step A in SH medium containing 2,4-D 2.0mg / L, in which sucrose 30g / L, agar powder 7.5g / L, pH5 .85;

[0039] C, callus induction: the explants obtained in step B are cultivated in 2,4-D 2.0-3.0mg / L, 0.5mg / L BA SH medium, wherein sucrose 30g / L, Agar powder 7.5g / L, pH5.80, 8 hours of light and 16 hours of darkness every day. Until the Chinese Lycoris bulb callus appears;

[0040] D. Callus culture: Inoculate the Chinese Ly...

Embodiment 2

[0044] A kind of method utilizing Chinese lycoris bulb callus to produce lycorine and ricolamin comprises the following steps:

[0045] A) Selection and sterilization of explants: take the bulbs of 'Chinese Lycoris' as explants, soak and rinse with 3% detergent solution, shake at 150 rpm for 20 minutes, rinse with water, and 75% alcohol disinfection for 30 seconds, 0.15% mercuric chloride sterilization treatment, soaking for 11 minutes, rinse with sterile water for 8 times, and set aside;

[0046] B. Inoculation of explants: Inoculate the explants prepared in step A in SH medium containing 2,4-D 2.0mg / L, in which sucrose 30g / L, agar powder 7.5g / L, pH5 .85;

[0047] C. Induction of callus: the explants obtained in step B are cultured in SH medium of 2,4-D 2.0mg / L, 0.5 mg / LBA, wherein sucrose 30g / L, agar powder 7.5 g / L, pH5.95, 8 hours of light and 16 hours of darkness every day. Until the Chinese Lycoris bulb callus appears;

[0048] D. Callus culture: Inoculate the Chinese...

Embodiment 3

[0052] A kind of method utilizing Chinese lycoris bulb callus to produce lycorine and ricolamin comprises the following steps:

[0053] A) Selection and sterilization of explants: take the bulbs of 'Chinese Lycoris' as explants, soak and rinse with 3% detergent solution, shake at 120 rpm for 20 minutes, rinse with water, and 75% alcohol disinfection for 30 seconds, 0.15% mercuric chloride sterilization treatment, soaking for 11 minutes, rinse with sterile water for 7 times, and set aside;

[0054] B. Inoculation of explants: Inoculate the explants prepared in step A in SH medium containing 2,4-D 3.0mg / L, wherein sucrose 30g / L, agar powder 7.5g / L, pH5 .8;

[0055] C. Induction of callus: the explants obtained in step B are cultured in SH medium of 2,4-D3.0mg / L, 0.5 mg / LBA, wherein sucrose 30g / L, agar powder 7.5 g / L, pH5.90, 8 hours of light and 16 hours of darkness every day. Until the Chinese Lycoris bulb callus appears;

[0056] D. Callus culture: Inoculate the Chinese Ly...

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Abstract

The invention provides a method for producing lycorine and lycoramine from Chinese lycoris calluses. 'Chinese lycoris' bulbs are used as explants, disinfection, sterilization and inoculation are conducted, after the bulbs are cultured for a certain period, calluses are produced at wounds of flakes, and proliferation culture is performed on the calluses continuously. The 'Chinese lycoris' calluses are dried, ground and subjected to ultrasonic extraction three times by the aid of methyl alcohol, and a methyl alcohol solution containing the lycorine and the lycoramine is obtained. With the method, the problems that Chinese lycoris resources are in shortage and chemical synthesis of the lycorine and the lycoramine is complex are solved by using the tissue culture technology. A used minimal medium takes an SH medium as a basis and is assisted by components such as 2,4-dichlorophenoxyacetic acid, L-proline, sucrose and the like; and a basis is provided for factory cell production of the lycorine and the lycoramine, the method is environment-friendly, raw materials are rich, and the problem that the market supply gap of the lycorine and the lycoramine is large can be well solved.

Description

technical field [0001] The invention relates to the production of lycorine and lycoclamine, in particular to a method for producing lycorine and lycoclamine through lycoris bulb callus. Background technique [0002] Lycoris, the chemical formula is C16H17NO4, the molecular weight is 287.30, and it is edge-shaped crystal. Melting point 275-280°C (decomposition). There is dextrorotation. Insoluble in water, hardly soluble in ethanol and ether. It is an alkaloid with emetic and expectorant effects isolated from the bulbs of Amaryllidaceae (Amaryllidaceae), and it is hydrogenated to generate dihydrolycorine, which has strong anti-amoeba dysentery Effect, and less toxic, has been used for clinical use, studies have shown that it has a protective effect on myocardial ischemia in mice; the endamine salt made of lycorine has anti-tumor effect. It has high development and utilization value. [0003] Lycoris (Lycoris), also known as lycoamine base, dihydrogalantamine. The chem...

Claims

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Application Information

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IPC IPC(8): C07D491/107C07D491/16A01H4/00
CPCA01H4/008A01H4/001C07D491/107C07D491/16
Inventor 穆红梅
Owner LIAOCHENG UNIV
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