Polyglutamic acid/sodium alginate adhesive hydrogel and preparation method thereof

A technology of sodium alginate and polyglutamic acid, applied in the field of polyglutamic acid/sodium alginate adhesive hydrogel and its preparation, can solve the problem of weakening material tissue adhesion, surrounding tissue toxicity, and limiting catechu Problems such as the application of phenol modified materials have achieved good application prospects and good biocompatibility

Active Publication Date: 2019-04-02
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Adhesive catechol residues are introduced into biocompatible polymer chains by chemical synthesis or modification, and then oxidative coupling of catechols is used to construct adhesive hydrogels into a However, this preparation method has obvious defects, such as the addition of sodium periodate will cause toxicity to surrounding tissues, and by-products will be generated during the oxidation process
Part of the catechol residues will be lost during the oxidative cross-linking process, which weakens the tissue adhesion of the material and limits the application of catechol-modified materials in tissue engineering

Method used

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  • Polyglutamic acid/sodium alginate adhesive hydrogel and preparation method thereof

Examples

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Embodiment 1

[0021] 1. Preparation of oxidized sodium alginate: Weigh 5.0g of sodium alginate into a 250mL beaker, add 150mL of deionized water, stir magnetically and heat to 60°C. After it is completely dissolved, cool to room temperature. Add 1.08 g of sodium periodate which is 0.2 times the amount of sodium alginate structural unit. After reacting for 3 hours under completely dark conditions, add 650ul ethylene glycol to terminate the reaction, stir for 20min and then settle in 2000mL ethanol to obtain a cotton-like solid, then dissolve the obtained solid with 200mL deionized water, and dialyze in deionized water for 3 days, freeze-dried to obtain oxidized sodium alginate with an oxidation degree of 19.4%.

[0022] 2. Preparation of catechol-modified oxidized sodium alginate: Weigh 0.2 g of oxidized sodium alginate prepared in step 1 into a 100 mL beaker, add 40 mL of deionized water, and add appropriate amount of EDC / NHS after fully dissolving the system. Adjust the pH value to about ...

Embodiment 2

[0027] The present embodiment is basically the same as the first embodiment, except that the amount of dopamine hydrochloride in step 2 is reduced from 0.6 g to 0.4 g, and the amount of other reagents and operating conditions remain unchanged. The modification rate of the obtained catechol-modified oxidized sodium alginate was 9%, the elastic modulus of the obtained gel was 1500Pa-1600Pa, and the bonding strength test: the lap shear modulus of cartilage slices was 28KPa.

Embodiment 3

[0029] This example is basically the same as Example 1, except that during the preparation of the poly-L-glutamic acid precursor solution in step 4, no FeCl was added 3 solution. Only 40 mg of ALG-CHO-DP solid was added to 800 uL deionized water to prepare a 5% solution, and the dosage and operating conditions of other reagents remained unchanged. The elastic modulus of the obtained gel was 1500Pa, and the bond strength test: the lap shear modulus of cartilage slices was 28KPa.

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Abstract

The invention relates to a polyglutamic acid / sodium alginate adhesive hydrogel and a preparation method thereof. Catechol groups with a tissue adhesion property are introduced in a poly(L-glutamic acid) / sodium alginate Schiff base gel system, and a first cross-linked network system is established through Schiff base crosslinking between hydrazide poly(L-glutamic acid) and oxidized sodium alginate; with the complexation of ferric ions as a second cross-linked network system, hydrogel is formed. Thus, catechol residues are retained as many as possible; meanwhile, a certain amount of ferric iron is mixed into the hydrazide poly(L-glutamic acid) component, the tissue adhesion of the catechol groups is exerted to the maximum, and tissue toxicity and other problems caused by conventional catechol oxidation crosslinking are avoided. The adopted materials have good biocompatibility, and various defects caused by oxidation crosslinking performed with oxidant are avoided; besides, adhesive elements are retained to the maximum, and the hydrogel has good application prospects in the tissue engineering field.

Description

technical field [0001] The invention relates to a polyglutamic acid / sodium alginate adhesive hydrogel and a preparation method thereof. Background technique [0002] Marine mussels can adhere to the surface of many organic and inorganic materials through silk. This special bioadhesion system has high strength, water resistance and self-healing functions. Its main component is rich in catechol structure Adhesin. Hydrogel, a kind of swellable 3D material, has received extensive attention in the field of tissue engineering in recent years. Adhesive catechol residues are introduced into biocompatible polymer chains by chemical synthesis or modification, and then oxidative coupling of catechols is used to construct adhesive hydrogels into a However, this preparation method has obvious defects, such as the addition of sodium periodate will cause toxicity to surrounding tissues, and by-products will be generated during the oxidation process. Part of the catechol residues will be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08G81/00C08G69/48C08G69/10C08B37/04
CPCC08B37/0084C08G69/10C08G69/48C08G81/00C08J3/075C08J2387/00
Inventor 颜世峰李星简宇航尹静波
Owner SHANGHAI UNIV
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