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Immobilized Bacillus having phosphate dissolving and heavy metal passivation functions and preparation method and application thereof

The technology of Bacillus cereus and Bacillus cereus is applied in the field of cadmium-contaminated soil bioremediation, and achieves the effects of low cost, good restoration and restoration effect, and increasing effective nutrient content.

Inactive Publication Date: 2017-04-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since most of the current microbial remediation products focus on the removal of heavy metals, few people have studied multifunctional strains, especially microbial preparations that can both promote growth and passivate heavy metals, which can increase production while removing heavy metals. Agricultural production safety is of great significance

Method used

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  • Immobilized Bacillus having phosphate dissolving and heavy metal passivation functions and preparation method and application thereof
  • Immobilized Bacillus having phosphate dissolving and heavy metal passivation functions and preparation method and application thereof
  • Immobilized Bacillus having phosphate dissolving and heavy metal passivation functions and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of Immobilized Bacillus with Phosphorus Solubilizing and Passivation Heavy Metal Functions

[0045] Activate Bacillus cereus, inoculate 1% of it into 30mL LB medium (beef extract peptone medium), shake at 150-180rpm at 30±2°C for 12-16h, prepare fermentation broth, and weigh 0.1g biological Charcoal is added to the fermentation broth, shaken at 30±2°C, 150-180rpm for 24h, taken out, centrifuged at 7000rpm for 5min, washed 2-3 times with sterile water, and the precipitate is collected to obtain immobilized spores with functions of decomposing phosphorus and passivating heavy metals bacilli.

Embodiment 2

[0046] Example 2 Adsorption Characterization of Immobilized Bacillus with Functions of Phosphorus Solubilization and Passivation of Heavy Metals

[0047] The biomass adsorbed on biochar was measured by lipophosphorus method, and Bacillus cereus was labeled with GFP, and whether the surface of biochar emitted green fluorescence was observed under a fluorescence microscope to characterize the situation of Bacillus cereus adsorbed on biochar.

[0048] 1. Determination of biochar by lipophosphorus method

[0049] (1) Use a 200-mesh nylon screen to separate the biochar carrier immobilized by microorganisms from the bacterial suspension and transfer it to a 100mL conical flask, and quickly add 10mL of chloroform, 20mL of methanol, and distilled water to the conical flask with a stopper. 8mL (volume ratio: 1:2:0.8) constitutes the extraction mixture of biolipid phosphorus, put the Erlenmeyer flask back into the constant temperature shaker and shake vigorously for 10min, take it out a...

Embodiment 3

[0077] Example 3 Phosphorus dissolving ability of the immobilized bacillus with functions of dissolving phosphorus and passivating heavy metals to inorganic phosphorus medium

[0078] Weigh 0.1 g of the prepared immobilized bacillus with the functions of dissolving phosphorus and passivating heavy metals and add it to the inorganic phosphorus liquid medium (calcium phosphate, iron phosphate and aluminum phosphate medium, replace calcium phosphate with iron phosphate and aluminum phosphate as the only Phosphorus source. Inorganic phosphorus liquid medium formula: glucose 10g, calcium phosphate 5g, magnesium chloride 5g, magnesium sulfate 0.25g, potassium chloride 0.2g, ammonium sulfate 0.1g, distilled water 1 000mL, pH 7.0), and wax-only samples Bacillus B19 treatment, only biochar treatment and blank control, 30±2°C, 150-180rpm shaker for 72h, take out 7000rpm and centrifuge for 5min, take the supernatant to measure the amount of phosphorus dissolved, and study its ability to d...

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Abstract

The invention discloses an immobilized Bacillus having phosphate dissolving and heavy metal passivation functions and a preparation method and an application thereof, which belong to the technical field of cadmium pollution soil biological restoration. The immobilized Bacillus comprises biological carbon and Bacillus cereus B19, can be used for restoring the soil with severe cadmium pollution, and has better restoration effect. The composite microbe fertilizer contains the immobilized Bacillus, an organic fertilizer and an inorganic nutrient having the phosphate dissolving and heavy metal passivation functions. The composite microbe fertilizer has the advantages that cost is low, soil available nutrient content is increased, yield increase is achieved, the soil with cadmium pollution is effectively repaired, introduction of heavy metal cadmium in human body with crops can be effectively controlled, agricultural product safety is guaranteed; in addition, the composite microbe fertilizer cannot change a farmland utilization mode without secondary pollution, is conveniently used, and is economic and feasible.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of cadmium-contaminated soil, and in particular relates to an immobilized bacillus with functions of dissolving phosphorus and passivating heavy metals, and a preparation method and application thereof. Background technique [0002] Cadmium (Cd) is a heavy metal element with strong biological toxicity. It has strong chemical activity, high mobility, and long-lasting toxicity in the environment. It is easy to endanger human health through the enrichment of the food chain, and can induce liver, kidney, and bone lesions. Also carcinogenic. There are physical remediation, chemical remediation and biological remediation methods for remediation of soil contaminated by heavy metals such as cadmium. Physical restoration mainly includes foreign soil, soil leaching and electric restoration. Although the restoration effect is good, the restoration cost is high and it is difficult to promote it on a l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C05G3/04B09C1/10C12R1/085C05G3/80
CPCB09C1/10C05G3/80C12N11/14
Inventor 蒲强徐会娟李永涛彭桂香蔡燕飞陈豪
Owner SOUTH CHINA AGRI UNIV
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