Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aldosterone-producing adenoma related up-regulation lncRNA and applications thereof

A technology of adrenal aldosterone adenoma and adrenal aldosterone gland, applied in the direction of DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc.

Inactive Publication Date: 2017-04-26
SOUTHERN MEDICAL UNIVERSITY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pathogenesis of adrenal aldosteronoma (APA) is still in the stage of research and exploration, and whether lncRNA is involved in the occurrence, development and regulation mechanism of APA has not yet been involved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aldosterone-producing adenoma related up-regulation lncRNA and applications thereof
  • Aldosterone-producing adenoma related up-regulation lncRNA and applications thereof
  • Aldosterone-producing adenoma related up-regulation lncRNA and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1. Strictly select 3 tissue samples from APA cases and 3 normal adrenal tissue control samples. A series of indicators such as medical history, clinical manifestations, biochemical examination, and imaging examination were used to diagnose APA cases before operation; pathological sections were used to further confirm the diagnosis of selected case specimens after operation. In addition, the selected adrenal glands excised during radical nephrectomy, which were confirmed by pathological sections as normal adrenal gland tissues, were used as control samples (see Table 1, figure 1 , figure 2 ).

[0018] Table 1: Basic information of patients

[0019]

[0020] Basic process of Agilent mRNA single-channel expression profiling chip experiment

[0021] (1) Extraction of tissue block and cellular RNA

[0022] Using a suitable extraction method, such as Trizol (Invitrogen, Gaithersburg, MD, USA), etc., the total RNA in tissue blocks or cells was extracted. and further a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Amplified lengthaaaaaaaaaa
Amplified lengthaaaaaaaaaa
Amplified lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses an aldosterone-producing adenoma related up-regulation lncRNA and applications thereof. According to the present invention, the aldosterone-producing adenoma related up-regulation lncRNA is found through the experiment; and by quantitating the expression of lncRNA, the auxiliary diagnosis of APA can be well achieved, the diagnose basis can be provided for the early diagnosis, the early surgery and the complete cure of part of refractory hypertension patients caused by primary aldosteronism (PA), and the treatment of high blood pressure can be effectively guided.

Description

technical field [0001] The present invention relates to a molecular diagnostic marker, in particular to an up-regulated lncRNA related to adrenal aldosterone adenoma and its application. Background technique [0002] In recent years, some studies have reported that the prevalence of primary aldosteronism (PA) is 5% to 15% in hypertensive patients, and 17% to 20% in patients with refractory hypertension [Mulatero P, Dluhy RG, Giacchetti G, Boscaro M, Veglio F, Stewart PM. Diagnosis of primary aldosteronism: fromscreening to subtype differentiation. Trends Endocrinol Metab. 2005;16:114–119.]. If the disease accounts for 6% of the hypertensive population, 9.6 million of the 160 million hypertensive patients in my country are caused by PA. Therefore, this has become a major issue destabilizing the prevalence and diagnosis of essential hypertension. However, it is estimated that less than 10,000 PA patients are confirmed each year in my country. If so many PAs are classified as...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12Q1/68
CPCC12N15/113C12N2310/10C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 陈英华张利朝胡卫列
Owner SOUTHERN MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com