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A kind of tissue culture rapid propagation method of seedling medicine Teng

A technology of tissue culture and rapid propagation of A. japonica, which is applied in the field of plant tissue culture, can solve the problems of no patent application for tissue culture and rapid propagation of s. , low cost, strong technical effect

Inactive Publication Date: 2018-08-17
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the tissue culture technology of A. japonica at home and abroad, and there is no application for a patent for tissue culture and rapid propagation of A. chinensis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Explant collection: In the wild, select the middle and upper part of the plant of the black bone vine with no obvious diseases, and the sunny and substantial cane with joints as explants. After collection, water retention and moisturizing treatment should be carried out immediately and brought back to the laboratory in time. .

[0021] (2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 15 seconds, rinsed with sterile water for 5 seconds After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 15 minutes, wash it with sterile water for 5 times, dry it with sterile filter paper, and cut it into 1.5-2.5cm vines with knots. The stems are inoculated into the induction medium, placed at 25° C. for 30 days in full dark culture to induce the formation of adventi...

Embodiment 2

[0027] (1) Explant collection: In the wild, select the middle and upper part of the plant of the black bone vine with no obvious diseases, and the sunny and substantial cane with joints as explants. After collection, water retention and moisturizing treatment should be carried out immediately and brought back to the laboratory in time. .

[0028] (2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 17 seconds, rinsed with sterile water for 6 After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 18 minutes, wash it with sterile water for 6 times, dry it with sterile filter paper, and cut it into 1.5-2.5cm vines with knots. The stems are inoculated into the induction medium, placed at 27°C for 33 days in full dark culture to induce the formation of adventitious buds...

Embodiment 3

[0034] (1) Explant collection: In the wild, select the middle and upper part of the plant of the black bone vine with no obvious diseases, and the sunny and substantial cane with joints as explants. After collection, water retention and moisturizing treatment should be carried out immediately and brought back to the laboratory in time. .

[0035](2) Explant induction: when step (1) is collected back to the laboratory, the explants are first rinsed under tap water overnight, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 20 seconds, rinsed with sterile water for 7 After the first time, dry the water with sterile filter paper, sterilize it in 0.1% mercuric chloride solution for 20 minutes, wash it with sterile water for 7 times, dry it with sterile filter paper, and cut it into 1.5-2.5cm vines with knots. The stems are inoculated into the induction medium, placed at 28° C. for 35 days in full dark culture to induce the formation of adventitious bud...

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PUM

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Abstract

The invention relates to a plant tissue culture method in an agricultural biotechnology, in particular to a tissue culture and rapid propagation method of a Miao medicine periploca forrestii schltr. The method sequentially comprises the following steps of acquiring an explant of the periploca forrestii schltr; inducing the explant; performing multiplication culturing of adventitious buds; rooting culturing the adventitious buds; transplanting test-tube plantlets, wherein an induction medium for explant inducing comprises 3.0 to 5.0mg / L of MS culture medium, 1.5 to 2.0mg / L of KT, 20 to 30g / L of 6-BA, 3.5 to 4.0g / L of saccharose, 0.5 to 1.0 g / L of agar, and activated carbon with the pH being ranged from 5.4 to 5.8. According to the method provided by the invention, the transplanting survival rate of the Miao medicine periploca forrestii schltr achieves not less than 95 percent.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of the seedling medicine Acanthus fragrans. Background technique [0002] Miao medicine Periploca forrestil Schltr, also known as black keel, Diangangliu, etc., is a vine-like shrub of the genus Periploca in the Ascariaceae family. It is widely used by the Miao people to treat closed soft tissue injuries, rheumatism and rheumatoid diseases. It is rich in C21 steroids, cardiac glycosides, terpenes and other substances. As a kind of Miao medicine, black bone vine has a long history. As early as in the Western Han Dynasty Liu Xiang's "Shi Shuo Xin Yu Shuo Yuan Bian Wu": "I heard that the ancient doctor was a doctor, called Miao Fu, with pure medical skills and prescriptions. It is unique and praised by the world", and there is a description in the "Maguan County Chronicles and Customs" in the Qing Dy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁钧淞杨业容莫昭展韦敏
Owner YULIN NORMAL UNIVERSITY
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