A general dna extraction method and kit for molecular identification of animal traditional Chinese medicine

Abstract

The invention discloses a general DNA extraction method for identification of animal traditional Chinese medicine molecules. The method comprises the following steps: a step 1, lysate PE is prepared; a step 2, pretreatment of an animal traditional Chinese medicine sample is carried out; a step 3, extraction of an animal traditional Chinese medicine DNA is carried out; the step 3, 1, the sample after pretreatment in the step 2 and lysate PE prepared in the step 1 are mixed; the step 3, 2, broad spectrum protease is added, phenol chloroform is used for extracting in order to remove protein and other impurities; the step 3, 3, a precipitating agent is used in order to obtain the animal traditional Chinese medicine DNA. The invention also discloses a general DNA extraction kit for identification of animal traditional Chinese medicine molecules. The DNA extraction method and the kit are used for extracting DNA of animal medicines of various pharmacy parts except shell kind, secretion kind, and partial processed goods; obtained DNA satisfies subsequent experiment requirements of traditional Chinese medicinal material molecule identification, and promotion and application of Chinese medicinal material molecule identification are guaranteed.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a general DNA extraction method and a kit for molecular identification of animal traditional Chinese medicines. Background technique [0002] Animal traditional Chinese medicine is an important part of traditional Chinese medicine. According to the results of the third national survey of traditional Chinese medicine resources, there are 1581 kinds of medicinal animals in my country. At present, 77 kinds of medicinal animals and 50 kinds of animal medicines are included in the 2015 edition of "Chinese Pharmacopoeia" (Part One). Animal medicine has the characteristics of "strong activity, high curative effect, and great benefit", and is often used clinically to treat difficult and miscellaneous diseases, acute and severe diseases. Most animal medicines come from complex sources, mostly taken from a certain part of the animal, and there are often many broken organs and tissues, whi...

AI Technical Summary

Problems solved by technology

At present, kits are often used for animal drug DNA extraction, and different kits need to be selected according to the different parts of the drug: snakes, geckos, earthworms and other medicinal materials are rich in muscle tissue, and the use of general-purpose animal tissue DNA extraction kits can obtain higher DNA extraction kits. High-quality DNA; due to the low DNA content of medicinal materials such as horns and bone shells, special DNAout kits are required to extract DNA; however, the use of kits to extract DNA from complex animal drugs is often limited, such as mulberry octopus, frog oil, etc. The DNA of medicinal materials is difficult to obtain, and is limited to the identification of the original species

At the same time, the inconsistent DNA extraction methods for animal drugs hinder the application of automated DNA extraction for animal drugs, and limit the development of molecular identification technology for Chinese patent medicines containing mixed animal drug ingredients.

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