Method for simultaneously detecting plurality of types of taxanes

A technology of taxanes and 10-DAB, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of many types and dosage of organic reagents, inability to detect taxanes, high detection limit concentration, etc., and achieve the lowest reduction The detection limit concentration, widening the applicable concentration range, and improving the effect of resolution

Inactive Publication Date: 2017-05-10
TIANJIN ACELBIO BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, in the process of simultaneous detection of taxanes, "Establishment of a liquid mass spectrometry method for the simultaneous detection of multiple taxanes in Chinese yew" "HPLC method for the determination of the content of taxanes in different parts of the southern yew " and other literatures use many types and dosages of organic reagents, the detection cost is relatively high, the minimum detection limit concentration is high, and low concentration taxanes cannot be detected at the same time

Method used

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  • Method for simultaneously detecting plurality of types of taxanes
  • Method for simultaneously detecting plurality of types of taxanes
  • Method for simultaneously detecting plurality of types of taxanes

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Embodiment 1

[0036] The present invention configures standard solutions of 10-DAB, BAT III and Taxol with different concentrations through ethanol, and filters them through a 20 μm filter membrane. Detection instrument: Waters ACQUITY UPLC, chromatographic column: Agilent Poroshell 120 EC-C18 2.1 x100 mm, 2.7 μm, Column temperature: 40°C, flow rate: 0.5 ml / min, sample cell temperature: 10°C, set the mobile phase gradient as shown in Table 4, and obtain the separation chromatogram of the standard under these conditions, as shown in figure 2 Shown:

[0037] Table 4 Mobile phase gradient elution condition settings:

[0038]

Embodiment 2

[0040] Carry out 10-DAB, BAT III and Taxol three kinds of taxane standard substance detections in the method described in embodiment 1, obtain relevant methodological data as shown in table 5:

[0041] Table 5 Methodological data of 10-DAB, BAT III and Taxol three taxanes:

[0042]

Embodiment 3

[0044] Compared with the current HPLC detection method, the present invention can obviously shorten the detection time and reduce the minimum detection limit concentration. The comparison results are as shown in Table 6:

[0045]Table 6 conventional HPLC method compares with the present invention:

[0046]

[0047]

[0048] The results show that when using HPLC or ES-MS to detect taxanes, the detection time is longer. The retention time of paclitaxel in the current detection method is more than 20 min. After using this method, the separation degree of taxanes is improved. The retention time of paclitaxel was shortened to 8.8 min, the efficiency was increased by more than 50%, and the minimum detection limit concentration could be reduced at the same time.

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Abstract

The invention discloses a method for simultaneously detecting a plurality of types of taxanes. The detection method comprises the following steps: (1) preparing a plurality of types of taxane samples by adopting ethanol, methanol or acetonitrile; carrying out membrane filtration or centrifuging and taking supernatant; and taking filtrate or the supernatant as a sample solution to be detected; and (2) determining the content of taxanes components in the sample solution to be detected by adopting a UPLC (Ultra Performance Liquid Chromatography) method. The UPLC method established by the invention can be used for improving the separation degree of simultaneous detection of the plurality of types of taxanes, the detection time is reduced and the lowest detection limit concentration is reduced; and the lowest detection limit concentrations of 10-DAB (10-Deacetylbaccatin), BAT III and Taxol are 0.14 microgram / milliliter, 0.13 microgram / milliliter and 0.07 microgram / milliliter respectively. Compared with a current method for simultaneously detecting the plurality of types of taxanes, the method has obvious advantages.

Description

technical field [0001] The invention belongs to the technical field of using instruments to detect chemical components, and relates to a method for simultaneously detecting multiple taxanes. Background technique [0002] Taxanes are a large class of natural products obtained from plants. They are a group of terpenoid small molecule active substances with tricyclic pentamethylpentadecene skeletons, including paclitaxel (Taxol) and their The semi-synthetic precursors 10-deacetylbaccatin III (10-DAB) and baccatin III (BAT III), etc., are specific secondary metabolites of Taxus plants. The most famous taxane substance is paclitaxel, which was approved by the US FDA in 1992 for the treatment of ovarian cancer. In 2011, the international market sales of paclitaxel preparations were 3.7 billion US dollars, and it is currently the main drug in the field of cancer treatment. With the focus on taxanes, more than 400 taxanes have been identified from various yew plants and cell cultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘永彬翟丽娟其他发明人请求不公开姓名
Owner TIANJIN ACELBIO BIOTECH
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