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A composition for detecting donkey-hide gelatin content in donkey-hide gelatin cake and its detection method

A technology of donkey-hide gelatin cake and content, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of quantitative detection of donkey skin source components, discounts on accuracy and reliability, and supervision of Ejiao products in the unfavorable market.

Active Publication Date: 2019-11-29
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The two reported methods are unable to carry out more accurate quantitative detection of donkey-hide gelatin-derived components, but to determine the authenticity of donkey-hide gelatin by detecting whether donkey-hide gelatin contains donkey components and excluding other animal-derived components; but the donkey-hide gelatin Adulteration may be varied, so there will be a variety of animal-derived ingredients that need to be excluded. There are important flaws in using the exclusion method to identify the authenticity of donkey-hide gelatin, and its accuracy and reliability will be greatly reduced. This situation is very unfavorable to the market for donkey-hide gelatin. regulation of products

Method used

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  • A composition for detecting donkey-hide gelatin content in donkey-hide gelatin cake and its detection method
  • A composition for detecting donkey-hide gelatin content in donkey-hide gelatin cake and its detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1 Materials and Reagents

[0034] Materials: All kinds of pure glue, including donkey-hide gelatin, horsehide glue, yellow gelatin, pigskin glue, sheepskin glue, fish skin glue (the reference substance detection matrix), donkey skin, horse hide, cowhide, pigskin, sheepskin, Obtained by decocting fish skin.

[0035] Donkey-hide gelatin cake: made by adding rice wine, rock sugar, black sesame, and walnut kernels to the above-mentioned pure glue, including a donkey-hide gelatin cake sample made of donkey-hide gelatin (the donkey-hide gelatin accounts for about 23% of the total weight of the donkey-hide gelatin cake), horse hide The fake donkey-hide gelatin cake made from gelatin (the content of donkey-hide gelatin is 0%), the fake donkey-hide gelatin cake made from yellow gelatin (the donkey-hide gelatin content is 0%), the fake donkey-hide gelatin cake made from pig skin glue (the donkey-hide gelatin content is 0%) The content is 0%), and the fake donkey-hide gelatin cak...

Embodiment 2

[0059] 1 Materials and Reagents

[0060] Material:

[0061] The mixed gum samples were prepared by adding different qualities of donkey-hide gelatin to the pure gum sample in Example 1, including yellow gelatin containing 15% donkey-hide gelatin, yellow gelatin containing 30% donkey-hide gelatin, and yellow gelatin containing 60% donkey-hide gelatin.

[0062] Donkey-hide gelatin cake: made by adding rice wine, rock sugar, black sesame, and walnut kernels to the above-mentioned mixed gum samples, including donkey-hide gelatin cake samples made from yellow gelatin with 15% donkey-hide gelatin (the donkey-hide gelatin accounts for about 3.5% of the total weight of the donkey-hide gelatin cake) ), a donkey-hide gelatin cake sample made with 30% donkey-hide gelatin yellow gelatin (the donkey-hide gelatin accounts for about 6.9% of the total weight of the donkey-hide gelatin cake), a donkey-hide gelatin cake sample made with 60% donkey-hide gelatin yellow gelatin (the donkey-hide ge...

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Abstract

The invention discloses a composition and detection method for detecting the content of donkey-hide gelatin in donkey-hide gelatin cakes. The composition is composed of a polypeptide I, a polypeptide II, a polypeptide III and a reference substance detection matrix; the amino acid sequence of the polypeptide I is shown as SEQ ID NO. 1; the amino acid sequence of the polypeptide II is shown as SEQ ID NO. 2; the amino acid sequence of the polypeptide III is shown as SEQ ID NO. 3; the reference substance detection matrix is fish skin glue. The composition can be used for detecting the content of donkey skin source components in the donkey-hide gelatin cakes, so that whether the donkey-hide gelatin is faked or not is judged. A detection value of the polypeptide II is subtracted from the detection value of the polypeptide I and detection deviation is corrected by utilizing the detection value of the polypeptide III, so that the detection of the content of the donkey skin source components in the donkey-hide gelatin cakes is realized. The method has the advantages of accuracy in content detection and simplicity in operation and has a wide application prospect.

Description

technical field [0001] The invention relates to a composition of donkey-hide gelatin content in donkey-hide gelatin cake and a detection method thereof, belonging to the technical field of medicine and food detection. Background technique [0002] As the saying goes: "Tonic in autumn and winter, to fight tigers in the coming year." Donkey-hide gelatin is a dual-purpose food material, which has the functions of nourishing yin and blood, and is a good tonic for autumn and winter. The main eating method of donkey-hide gelatin is to boil donkey-hide gelatin with rice wine, add black sesame seeds, walnut kernels, etc., and process it into donkey-hide gelatin cake for consumption. However, as the price of donkey-hide gelatin has risen year by year, many lawbreakers have appeared in the market. When producing donkey-hide gelatin, some or all of them are cooked with various other animal skins, including horse hide, mule hide, cowhide, pig skin, etc., instead of donkey hide. Some a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/042
Inventor 周祥山郝向慧郭尚伟段小波徐云鹏王静王玉娇石永坚郑海荣
Owner SHAN DONG DONG E E JIAO
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