Composition for identifying authenticity of donkey-hide gelatin and compound preparation thereof, kit and detection method thereof

A compound preparation and authenticity identification technology, applied in the field of medical testing, can solve the problems of unfavorable market donkey-hide gelatin product supervision, accuracy and reliability discount, quantitative detection of donkey skin source components, etc.

Active Publication Date: 2017-05-24
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The two reported methods are unable to carry out more accurate quantitative detection of donkey-hide gelatin-derived components, but to determine the authenticity of donkey-hide gelatin by detecting whether donkey-hide gelatin contains donkey components and excluding other animal-derived components; but the donkey-hide gelatin Adulteration may be

Method used

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  • Composition for identifying authenticity of donkey-hide gelatin and compound preparation thereof, kit and detection method thereof
  • Composition for identifying authenticity of donkey-hide gelatin and compound preparation thereof, kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1 Materials and Reagents

[0038] Materials: All kinds of pure glue, including donkey-hide gelatin, horsehide glue, yellow gelatin, pigskin glue, sheepskin glue, fish skin glue (the reference substance detection matrix) respectively use donkey hide, horsehide, cowhide, pigskin, sheepskin, fish skin Obtained by decocting the skin.

[0039] Peptide I, Peptide II, and Peptide III are synthesized by biological companies.

[0040] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure)

[0041] 2 detection method

[0042] 1) Preparation of matrix solution:

[0043] Weigh 0.50g of the reference substance detection matrix into a 250ml volumetric flask, add a small amount of 1% (w / w) NH 4 HCO 3 solution (pH8.0), ultrasonically dissolve the sample completely, and use 1% (w / w) NH 4 HCO 3 Solution (pH8.0) diluted to the mark, shake well.

[0044] 2) Preparation of reference substance mother liquor:

[0045] Weigh 0.05g of the reference substance detection ...

Embodiment 2

[0062] 1 Materials and Reagents

[0063] Material:

[0064] The mixed gum samples were prepared by adding different qualities of donkey-hide gelatin to the pure gum sample in Example 1, including yellow gelatin containing 15% donkey-hide gelatin, yellow gelatin containing 30% donkey-hide gelatin, and yellow gelatin containing 60% donkey-hide gelatin.

[0065] Samples of donkey-hide gelatin compound preparations: extract the concentrated solution from the plant medicinal materials in the production process of compound donkey-hide gelatin pulp, add the above-mentioned mixed gums respectively, add 1.0 g of the mixed gum samples to every 9 g of the plant medicinal materials extracted concentrated solutions, and make 3 compound gum-like traditional Chinese medicine preparations, respectively They are named as preparation 1, preparation 2 and preparation 3, and the content of donkey skin source components are 1.5%, 3.0% and 6.0% respectively.

[0066] Polypeptide I, polypeptide II,...

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Abstract

The invention discloses a composition for identifying the authenticity of donkey-hide gelatin and a compound preparation thereof, a kit and a detection method thereof. The composition is prepared from a polypeptide I, a polypeptide II, a polypeptide III and a reference substance detection matrix, and accordingly the authenticity of the donkey-hide gelatin is judged by utilizing donkey-hide source ingredient contents in the donkey-hide gelatin and the compound preparation thereof. An amino acid sequence of the polypeptide I is shown as SEQIDNO.1, an amino acid sequence of the polypeptide II is shown as SEQIDNO.2, an amino acid sequence of the polypeptide III is shown as SEQIDNO.3, and the reference substance detection matrix is fish skin glue. The detection of the donkey-hide source ingredient contents in the donkey-hide gelatin and the compound preparation thereof is achieved by subtracting a polypeptide II detection value from a polypeptide I detection value and utilizing a polypeptide III detection value to correct detection deviations. The method has the advantages of being accurate in content detection and simple in operation and has a wide application prospect in the field of donkey-hide gelatin product quality monitoring.

Description

technical field [0001] The invention relates to a composition, a kit and a detection method for authenticating donkey-hide gelatin and its compound preparations. The invention belongs to the technical field of medical detection. Background technique [0002] Ejiao, as a traditional precious Chinese medicine, has been eaten for thousands of years. It has the functions of nourishing blood, nourishing yin, moistening dryness and stopping bleeding. The Chinese Pharmacopoeia stipulates that donkey-hide gelatin is a solid gelatin obtained by decocting and concentrating the dried skin or fresh skin of Equus asinus L., an equine animal donkey. Pure donkey-hide gelatin must come from 100% donkey skin to ensure the efficacy of the product. From the perspective of raw material taxonomy, donkeys belong to the genus Equus of the order Perissodactyla, including African wild asses, Tibetan wild asses, Central Asian wild asses, and domestic asses. Domestic donkeys raised in China include...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 秦玉峰郝向慧郭尚伟段小波徐云鹏王静王玉娇石永坚周祥山孙方玉
Owner SHAN DONG DONG E E JIAO
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