Application of Bacillus in degrading aromatic compounds

A technology of aromatic compounds and bacillus, applied in the field of microorganisms, can solve the problems of incomplete pollution, secondary pollution by chemical methods, changes in locations, etc., and achieve the effects of low cost, no secondary pollution, and high degradation efficiency

Inactive Publication Date: 2017-05-24
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These treatment methods have their own advantages and disadvantages, among which physical methods usually include coagulation precipitation, heating method, adsorption method, extraction method, washing method, etc., which are relatively simple to operate and more suitable for industrial wastewater with high concentration of benzene ring compounds Treatment of waste liquid and accidental pollution, but the limitation of this method is that it faces the challenge of high ene...

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  • Application of Bacillus in degrading aromatic compounds
  • Application of Bacillus in degrading aromatic compounds
  • Application of Bacillus in degrading aromatic compounds

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: bacillus L1 ( Bacillus ligniniphilus L1) Degradation effect on 3,4-dihydroxybenzoic acid

[0029] Bacillus L1 ( Bacillus ligniniphilus L1) was isolated and screened from the seabed mud of the South China Sea, and its isolation, screening and identification process are detailed in the article: (Zhu D , Tanabe SH , Xie C , Honda D , Sun J , AiL.Bacillus ligniniphilus sp. nov., an alkaline and halotolerant bacteria isolated from sediments of the South China Sea. International Journal of Systematic and Evolutionary Microbiology. DOI: 10.1099 / ijs.0.058610-0).

[0030] The detailed process descriptions such as the screening, isolation and identification of the bacteria have been published, and are materials well known to those skilled in the art.

[0031]This example investigates the degradation of Bacillus L1 to different concentrations of 3,4-dihydroxybenzoic acid. The main steps are as follows: the bacteria were enriched in the enrichment medium (tryptone...

Embodiment 2

[0037] Embodiment 2: bacillus L1 ( Bacillus ligniniphilus L1) Degradation effect of 3-methoxy-4-hydroxybenzoic acid

[0038] The main steps are the same as in Example 1, and the inoculum size of 3% (V / V) is inoculated to the sterilized initial concentration of 100, 500, 1000, 1500 and 2000 mg / L of 3-methoxy-4-hydroxyl respectively. Benzoic acid is used as the only carbon source in the inorganic salt medium, and the temperature is 28° C., and the rotation speed is 180 rpm. Such as Figure 4 Be the degradation curve of the 3-methoxyl-4-hydroxybenzoic acid of 500mg / L for Bacillus in different time periods, Figure 4 It can be seen that there is a characteristic absorption peak at 275nm, and the concentration c is linearly correlated with the absorbance A, c=352.11A+17.43, using ultrapure water as a control, the absorbance at 275nm is used as the measurement index of p-hydroxybenzoic acid concentration and calculated Degradation rate.

[0039] The result is as Figure 9 show...

Embodiment 3

[0040] Embodiment 3: bacillus L1 ( Bacillus ligniniphilus L1) degradation effect of catechol

[0041] The main steps are the same as in Example 1, and the catechol of 100, 500, 1000, 1500, and 2000 mg / L is inoculated to the sterilized initial concentration as the only carbon source inorganic In the salt medium, shake culture at a temperature of 32° C. and a rotational speed of 150 rpm. Such as Figure 6 It is the degradation curve of 500mg / L catechol in different time periods for Bacillus, Figure 6 It can be seen that there is a characteristic absorption peak at 273nm, and the concentration c is linearly related to the absorbance A, c=490.20A+24.92. Taking ultrapure water as a control, the absorbance at 273nm is used as the measurement index of catechol concentration and calculated Degradation rate.

[0042] The result is as Figure 10 shown. Under the condition of 32°C, the degradation rates of catechol with the initial concentration of 100, 500, 1000, 1500 and 2000 m...

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Abstract

The invention provides application of Bacillus in degrading aromatic compounds, belonging to the technical field of microbes. The experiment proves that the Bacillus L1 strain has the capacity for degrading 3,4-dihydroxybenzoic acid, 3-methoxy-4-hydroxybenzaldehyde, catechol and other environmental pollutants; the degradation rates for para-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 3-methoxy-4-hydroxybenzoic acid, 3-methoxy-4-hydroxybenzaldehyde, catechol and benzoic acid with the concentration of 500 mg/L can respectively reach 90% or above; and thus, the Bacillus L1 strain has wide application prospects in the aspect of environmental treatment.

Description

technical field [0001] The invention relates to an application of bacillus in degrading aromatic compounds, which belongs to the technical field of microbes. Background technique [0002] Aromatic compounds are common pollutants in the environment. They mainly come from accidental leakage and release of by-products in industrial and agricultural industries such as pesticide production, printing and dyeing, papermaking, pharmaceuticals, and petroleum, as well as through certain biochemical effects. Aromatic compounds of relatively simple structure produced later are present in the environment. These widely distributed aromatic components, after a series of microbial oxidation and reduction, make intermediate metabolites of aromatic acids often detected in polluted waters, such as benzoic acid, o-, m-, p-toluic acid, etc. . According to reports, there are thousands of aromatic compounds entering the environment in my country every year. They mostly exist in the environment i...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/34C02F103/30
CPCC02F3/34C02F2101/308C02F2101/34C02F2103/30
Inventor 朱道辰张佩佩
Owner JIANGSU UNIV
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