Separation method of autotrophic bacteria and heterotrophic bacteria in activated sludge of sewage treatment plant

A sewage treatment plant, activated sludge technology, applied in the direction of biological water/sewage treatment, water/sludge/sewage treatment, separation of microorganisms, etc., to achieve the effect of convenient operation and high accuracy

Inactive Publication Date: 2017-05-24
BEIJING UNIV OF TECH
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Problems solved by technology

Then, molecular biology techniques were used to measure the microbial community structure of the two types of bacteria, and to analyze the functions of microorga

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[0038] 1. Stable isotope-labeled substrate culture activated sludge

[0039] (1) Prepare 2 kinds of substrates, respectively No. 1 substrate, containing 12 C-labeled sodium carbonate; matrix No. 2, containing 13 C-labeled sodium carbonate;

[0040] (2) Take the sludge mixed solution of the activated sludge system, wash and centrifuge three times with distilled water, and settle the sludge after centrifugation to half of the volume before elutriation;

[0041] (3) Take two 150mL Erlenmeyer flasks, add 50mL of elutriated sludge to each, and add No. 1 matrix respectively as the control group; add No. 2 matrix as the experimental group, keep the temperature at 25°C, and stir for 3 cycles , 10 hours per cycle. After the first two cycles, the centrifuged sludge was washed, and the volume of the sludge was adjusted to 50 mL, and the corresponding substrate was added to continue the next cycle of cultivation.

[0042] 2. Extraction of total DNA

[0043] Genomic DNA was extracted ...

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Abstract

The invention provides a separation method of autotrophic bacteria and heterotrophic bacteria in activated sludge of a sewage treatment plant, which belongs to the technical field of biological sewage treatment. According to the method, DNAs of the autotrophic bacteria and the heterotrophic bacteria with different demands for types of carbon sources in the activated sludge of the sewage treatment plant can be separated, and the characteristics of the two types of bacteria can be analyzed at a gene level. The activated sludge of the sewage treatment plant is cultivated in situ by adopting 13C-marked inorganic matters or organic matters as carbon sources, and DNA solutions at different levels are obtained by adopting a superspeed density gradient centrifugation method of cesium chloride with high stability and good separation effect. The densities of the solutions at all levels after separation are cut down from big to small correspondingly in the bottom-to-top direction of a centrifugal tube. The characteristics of microbial communities of DNA samples at 5-9 levels are analyzed by adopting a real-time quantitative PCR (polymerase chain reaction) technology and a high throughput sequencing technology. The invention provides the economic, simple, convenient and reliable separation method of the autotrophic bacteria and the heterotrophic bacteria in the activated sludge of the sewage treatment plant.

Description

technical field [0001] The invention relates to a method for separating autotrophic bacteria and heterotrophic bacteria in activated sludge of a sewage treatment plant, belongs to the technical field of sewage biological treatment, and is used for separating autotrophic bacteria and heterotrophic bacteria in sewage biological treatment systems. Background technique [0002] Sewage biological denitrification technology is widely used in the field of sewage treatment due to its economical and high-efficiency characteristics. Traditional sewage biological denitrification includes nitrification process and denitrification process. The nitrification process refers to the biological process in which free ammonia and ammonium salts are oxidized to nitrite and nitrate under aerobic conditions. The nitrification process is completed by specific chemoautotrophic bacteria. The carbon source required for biomass synthesis of chemoautotrophs comes from dissolved CO 2 , the energy come...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12Q1/68C02F3/34
CPCC02F3/34C02F2203/002C12N1/02C12Q1/689C12Q1/6895
Inventor 曾薇郭瑜李宁彭永臻
Owner BEIJING UNIV OF TECH
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