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Modified zeolite functional material for fixing benzene degrading bacteria and preparation method thereof

A technology for modifying zeolite and functional materials, which is applied in the directions of immobilization on or in inorganic carriers, chemical instruments and methods, water/sludge/sewage treatment, etc. , long life, high mechanical strength effect

Inactive Publication Date: 2017-05-24
光合强化(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immobilizing benzene-degrading bacteria is one of the most effective means to restore benzene-polluted environment. However, there is still a lack of functional materials for immobilizing benzene-degrading bacteria. Therefore, the development of modified zeolite functional materials for immobilizing benzene-degrading bacteria is of great importance It is of great significance to control and restore the environment polluted by benzene

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Add 45g of zeolite to 250mL of 15% ammonium dihydrogen phosphate solution, stir at 1000r / min for 50 minutes, filter to remove the liquid, wash with 500mL of deionized water and place in a drying oven at 102°C Stand for 45 minutes to obtain substance A;

[0031] (2) Add 8.6g of tetrahydropyrone into 400mL of absolute ethanol, stir for 8 minutes under the condition of 1000r / min, shake well and divide into 2 equal parts to obtain mixed solution M1 and mixed solution M2;

[0032] (3) Add 4.8 grams of 5-bromothiophene-2-carbaldehyde and 5.3 grams of 2,5-dichloro-3-bromopyridine into 150 mL of isopropanol, stir at 1000 r / min for 8 minutes, shake well and divide into 2 equal parts to obtain mixed solution N1 and mixed solution N2;

[0033] (4) Add the mixed solution M1 and 8.6 grams of tetrabromothiophene into the mixed solution N1, and stir for 8 minutes at 1000 r / min to obtain the mixed solution O1;

[0034] (5) Add substance A to the mixed solution O1, shake it in a s...

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Abstract

The invention discloses a modified zeolite functional material for fixing benzene degrading bacteria. Zeolite is modified by a mixed solution prepared from ammonium dihydrogen phosphate, tetrahydro-4H-pyran-4-one, 5-bromothiophen-2-formaldehyde, 2,5-dichloro-3-bromopyridine, tetrabromothiophene and 2-acetylene thiophene to obtain a substance B; the substance B is modified by a mixed solution prepared from 3-chloropyridazine, acetaldehyde phenylhydrazone, acetone oxime, guanidine nitrate and indole-6-carboxaldehyde to obtain a substance C; the substance C is modified by a mixed solution prepared from 3-formylthiophene, di-tert-amylbenzoquinone and 4-amino-3-hydroxy-1-naphthalene sulfonic acid to obtain a substance D; and the substance D is modified by a mixed solution prepared from propyl sulfate, 2-aminoanthraquinone and nitrourea to obtain a substance, namely the modified zeolite functional material for fixing the benzene degrading bacteria.

Description

technical field [0001] The invention belongs to the field of research and development and preparation of functional materials of environmental pollution control technology, and in particular relates to a modified zeolite functional material for immobilizing benzene-degrading bacteria and a preparation method thereof. Background technique [0002] Immobilized microorganism technology is a biotechnology developed from immobilized enzyme technology in biochemical industry in the 1960s. By chemical or physical means, free cells are positioned in a limited space area to keep them active and can be used repeatedly. In the late 1970s, with the increasingly serious water pollution, this technology was applied to the biological treatment of industrial wastewater due to its unique advantages. Compared with the traditional suspension biological treatment method, immobilized microorganism technology has the ability to improve the microbial Cell concentration, maintaining high-efficiency...

Claims

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Application Information

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IPC IPC(8): C12N11/14C02F3/34C02F101/32
CPCC12N11/14C02F3/34C02F2101/322
Inventor 不公告发明人
Owner 光合强化(北京)生物科技有限公司
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