A kind of biological emulsifier and its application

A biological emulsifier and emulsifier technology, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of slow recovery process efficiency, difficulty in fully meeting repair requirements, secondary pollution, etc., and achieve significant economic benefits and Large-scale application, good deoiling effect, and cost-saving effect

Active Publication Date: 2020-10-09
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing restoration technology and oil recovery process, there are certain limitations in physical and chemical restoration, and it is difficult to fully meet the restoration requirements, while the recovery process has problems such as slow efficiency and serious secondary pollution.

Method used

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  • A kind of biological emulsifier and its application
  • A kind of biological emulsifier and its application
  • A kind of biological emulsifier and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of emulsifying agent

[0024] 1) Preparation of crude extract

[0025] The active strain Geobacillus sp.XS2-450 was shaken at 60°C and 180rpm for 10 hours as a seed solution for fermentation, and the inoculation amount was 10% (v / v), inoculated into the fermentation medium, and the final concentration of 0.1 % palm oil is used as the inducer of producing highly active biological emulsifier, and the culture time of emulsifier fermented liquid is 24h, and the fermented liquid obtained is at 4 DEG C, centrifuged under the condition of 5000rpm for 30min to remove thalline, and the supernatant obtained is Crude extract of emulsifier. The crude extract was concentrated to 1 / 5 volume of the original supernatant, and stored at 4°C.

[0026] 2) Salting out treatment

[0027] Take 20ml of the emulsifier crude extract prepared in step 1) and add ammonium sulfate particles to make it reach the saturation of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% res...

Embodiment 3

[0037] Embodiment 3: the effect of the emulsifier prepared in embodiment 1

[0038] The present invention mainly utilizes the biological emulsifier prepared in Example 1 to treat oil sludge polluted by high viscosity and heavy hydrocarbons.

[0039] Stir the sludge to make it homogenized, adjust the ratio of sludge and bio-emulsifier to 4:5, add water 10 times that of the sludge, stir fully at 60°C to emulsify the crude oil in the sludge, and let it stand , the settled oil residue is deeply deoiled to remove residual high-viscosity components, so as to achieve the purpose of separating the oil and residue in the sludge, and finally recover all the emulsified upper crude oil. During the treatment process, the oil content of the sludge was monitored, and the oil content in the sludge was reduced from the original 26.2% to the current 1.5%, and the oil content was reduced by more than 94%. Figure 6 As shown, it meets the oil sludge disposal standard; compared with physical and ch...

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Abstract

The invention provides a biological emulsifier, which is prepared from the Bacillus licheniformis XS2-450 strain with the preservation number of CGMCC No.9430. The biological emulsifier obtained by the invention is used for treating oily sludge contaminated with high viscosity and heavy hydrocarbons. The emulsifying activity of the emulsifier obtained by the invention can reach 83.2%, and the emulsifying stability to liquid paraffin still maintains 72.8% after 30 days. At the same time, the biological emulsifier has a good deoiling effect when used in the treatment of high-viscosity and heavy hydrocarbon-contaminated sludge, which can reduce the oil content in the sludge from 26.2% to the current 1.5%, and the oil content is reduced by 94%. Above, compared with physical and chemical methods, the cost can be reduced by 30%, which shows great economic benefits and potential for large-scale application while saving costs and protecting the environment.

Description

technical field [0001] The invention belongs to the technical field of bioactive molecule preparation, and in particular relates to a bioemulsifier and its application. Background technique [0002] Bioemulsifier is a kind of amphiphile biomacromolecular compound with emulsifying activity. It is generally polymerized by sugar, lipid and protein. The molecules contain hydrophilic groups and hydrophobic groups. There are many types of bioemulsifiers with special functions and structures. It is a kind of biomacromolecular compound that exhibits important physiological and metabolic activities in the life process. Studies have shown that the emulsifying activity is closely related to the molecular structure of the emulsifier, especially the protein structure change has a great impact on the emulsifying activity. Influence, hydrophilic groups and hydrophobic groups are the basis of emulsifying activity, and the hydrophilic groups are generally polysaccharide structures, containin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/04C02F11/02C12R1/10C02F103/10
CPCC02F11/02C02F2103/10C12P1/04C12N1/205C12R2001/10
Inventor 高小龙王兴彪黄志勇董大媛
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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