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Method for extracting active protein from moringa oleifera seeds

A technology of active protein and extraction method, which is applied in the field of active protein extraction from Moringa oleifera seeds, can solve the problems of unsatisfactory pretreatment, complex process, and the extraction rate of active protein needs to be improved, so as to improve antioxidant properties and extract High rate and excellent effect

Inactive Publication Date: 2017-05-24
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The proteins and polypeptides prepared by the prior art have high biological activity, non-toxicity and good thermal stability, but the process is complex and the pretreatment is not ideal, and the extraction rate of active protein needs to be improved

Method used

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Examples

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Effect test

Embodiment 1

[0019] A method for extracting active protein from Indian moringa seeds, the steps are as follows:

[0020] 1) Pretreatment: Dry the Moringa seeds and peel them to obtain white spherical inner seeds, soak them in deionized water, dry them, ultrafinely pulverize them, and sieve them; deionized water can fully dissolve the peeled inner seeds Impurities are separated from internal molecules, avoiding the impact of impurities on the extraction in subsequent steps, and ensuring that there is no interference from impurities during the extraction process.

[0021] 2) Degreasing: add degreasing agent and active agent to the inner seed powder, stir, let it stand, filter to get a lumpy powder, then air-dry the powder and sieve; the oil in the inner seed will affect the extraction rate of active protein, so the degreasing agent is used for the extraction of active protein. The inner seed powder is degreased, and the added active agent can ensure the activity of the active protein in the ...

Embodiment 2

[0031] A method for extracting active protein from Indian moringa seeds, the steps are as follows:

[0032] 1) Pretreatment: Dry the Moringa seeds in the sun and peel them to obtain white spherical inner seeds. The inner seeds are soaked in deionized water for preferably 30 minutes, taken out, and then dried in a desiccator, which is equipped with a desiccant. The moisture in the Moringa oleifera seed powder is vaporized and escaped by heating to obtain the dried Moringa oleifera seed powder, which is then superfinely pulverized and 50-150 mesh sieved to obtain the Moringa oleifera seed powder, which is convenient for superfine pulverization and processing. Subsequent degreasing process, ultrafine grinding, sieving; deionized water can fully separate the soluble impurities on the surface of the peeled inner seeds from the inner seeds, avoiding the influence of impurities on the extraction in the subsequent steps, and ensuring that there is no interference of impurities in the e...

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Abstract

The invention provides a method for extracting active protein from moringa oleifera seeds. The method comprises steps as follows: pretreatment: the moringa oleifera seeds are dried in the sun and peeled off, white spherical inner seeds are obtained, soaked with deionized water, dried, subjected to superfine grinding and sieved; degreasing: a degreasing agent and an active agent are added to inner seed powder, the materials are stirred, left to stand and filtered, blocky powder is obtained, and the powder is air-dried and then is sieved; extraction of active protein: a Tris-HCl buffer solution is added to the sieved powder, a mixed solution is stirred and subjected to water-bath heating, an enzymolysis agent and a cosolvent are added, the solution is stirred continuously and centrifuged, a supernatant is taken, a precipitation agent is added to the supernatant, the mixture is left to stand and filtered, and active protein in the moringa oleifera seeds is obtained; the active protein is freeze-dried for storage. Efficient degreasing is realized through improvement of a pretreatment process, the extraction rate of active protein is remarkably increased, and the effect of the extracted active protein is excellent.

Description

technical field [0001] The invention belongs to the field of active protein extraction, in particular to a method for extracting active protein from Indian moringa seeds. Background technique [0002] Moringa seeds belong to the Moringaceae family of the cauliflower order, also known as the drumstick tree. Perennial tropical deciduous tree. In addition to a small amount of Moringa seeds being used for planting, a large amount of them are used as raw materials for production. They have good application value in food, medicine, health care, cosmetics and other fields, because they are rich in Moringa seed oil, which can reduce blood lipids. , blood pressure, weight loss, regulating the stomach, protecting the liver, abstaining from alcohol, and enhancing human immunity have significant curative effects. [0003] The existing technology provides a variety of active protein extraction methods, for example, the Chinese invention patent document A method for extracting natural a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06
Inventor 孙坤来陈子阳陈常健宋丽婉宣丹维董严杨帆王斌陈荫赵玉勤
Owner ZHEJIANG OCEAN UNIV
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