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A kind of method for extracting longan meat polysaccharide

A technology of longan meat polysaccharide and longan meat is applied in the field of deep processing of longan, which can solve the problems of high cost, low efficiency and long time, and achieve the effects of improving yield, improving enzymatic hydrolysis efficiency and improving enzymatic hydrolysis speed.

Active Publication Date: 2018-12-25
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing longan polysaccharide extraction technology takes a long time, and the cost of separation and preparation is high and the efficiency is low

Method used

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  • A kind of method for extracting longan meat polysaccharide
  • A kind of method for extracting longan meat polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preliminary preparation:

[0031] Preparation of copper oxide-zirconia catalyst: 35g of anhydrous copper sulfate and 30g of zirconium oxychloride were added to 200g of deionized water, and a sodium hydroxide solution with a mass fraction of 40% was added dropwise while stirring to adjust the pH value to 9. Filter, take the filter residue, wash twice with deionized water, dry in the air, microwave dry for 10 minutes, and finally bake in a muffle furnace at 1100°C for 2 hours to obtain a copper oxide-zirconia catalyst.

[0032] Preparation of lye: mix 10 g of potassium hydroxide, 10 g of ammonia water with a mass fraction of 25% and 100 g of deionized water, and stir evenly to obtain lye.

[0033]Preparation of ternary compound enzyme: 50 g of acid cellulase, 20 g of fructosidase and 15 g of protease were uniformly mixed to obtain a ternary compound enzyme.

[0034] Preparation of mixed alcohol solution: 1000g of 95% ethanol aqueous solution, 100g of 70% n-propanol aq...

Embodiment 2

[0042] 1. Preliminary preparation:

[0043] Preparation of copper oxide-zirconia catalyst: 38g of anhydrous copper sulfate and 35g of zirconium oxychloride were added to 240g of deionized water, while stirring, a sodium hydroxide solution with a mass fraction of 45% was added dropwise to adjust the pH value to 10, Filter, take the filter residue, wash with deionized water three times, dry in the air, microwave dry for 13 minutes, and finally bake in a muffle furnace at 1200°C for 2.5 hours to obtain a copper oxide-zirconia catalyst.

[0044] Preparation of lye: mix 10 g of potassium hydroxide, 18 g of ammonia water with a mass fraction of 25% and 120 g of deionized water, and stir evenly to obtain lye.

[0045] Preparation of ternary compound enzyme: 65 g of acid cellulase, 23 g of fructosidase and 20 g of protease were uniformly mixed to obtain ternary compound enzyme.

[0046] Preparation of mixed alcohol solution: 1200g mass fraction of 95% ethanol aqueous solution, 100g m...

Embodiment 3

[0054] 1. Preliminary preparation:

[0055] Preparation of copper oxide-zirconia catalyst: 43g of anhydrous copper sulfate and 37g of zirconium oxychloride were added to 260g of deionized water, and a sodium hydroxide solution with a mass fraction of 44% was added dropwise while stirring to adjust the pH value to 11. Filter, take the filter residue, wash with deionized water three times, dry in the air, microwave dry for 15 minutes, and finally bake in a muffle furnace at 1150°C for 3 hours to obtain a copper oxide-zirconia catalyst.

[0056] Preparation of lye: mix 10 g of potassium hydroxide, 24 g of ammonia water with a mass fraction of 25% and 130 g of deionized water, and stir evenly to obtain lye.

[0057] Preparation of ternary compound enzyme: 66 g of acid cellulase, 30 g of fructosidase and 26 g of protease were uniformly mixed to obtain ternary compound enzyme.

[0058] Preparation of mixed alcohol solution: 1450g mass fraction of 95% ethanol aqueous solution, 100g ...

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Abstract

The invention discloses a method for extracting longan pulp polysaccharide. The method comprises the following steps of (1) slurrying and slurry treatment: mixing fresh longan pulp and purified water, pouring a mixture into a homogenizer for slurrying, adding alkali liquor and activating at -4 to 10 DEG C for 10-12 hours to obtain an activated liquid; (2) enzymolysis and concentrating treatment: adjusting the pH value of filtrate, adding a copper oxide-zirconium oxide catalyst and ternary complex enzyme to filtrate, carrying out enzymolysis under the condition of 200-500W of microwave irradiation and then carrying out ultra-filtration concentration on enzymatic hydrolysate obtained through enzymolysis; (3) centrifugal treatment: mixing a concentrated liquid and a mixed alcohol solution, and collecting an educt by adopting a centrifugal machine; and (4) spray drying treatment. An alkali liquor low-temperature activating enzyme association technology is introduced into an extraction preparation process of the longan polysaccharide; the yield of a target product is obviously improved in comparison with a conventional technology; and meanwhile, the method has the characteristics of being efficient, fast, low in cost and suitable for massive industrial production.

Description

【Technical field】 [0001] The invention relates to the field of deep processing of longan, in particular to a method for extracting longan meat polysaccharide. 【Background technique】 [0002] Longan is the fruit of Sapindaceae, also known as longan, which is a famous subtropical fruit in my country. Longan is native to the subtropical region in southern my country and northern Vietnam. It has been cultivated in my country for more than 2,000 years. It is an important evergreen longevity fruit tree in the subtropical region. It has been known as "longan" in the south and "ginseng" in the north in history. Longan fruit sells well at home and abroad and enjoys a high reputation in the international market. my country's longan cultivation area and output rank first in the world. my country's Fujian, Guangdong, Guangxi and Taiwan provinces are the main production areas, and Hainan, Yunnan, Sichuan and Guizhou are also cultivated. [0003] Longan meat polysaccharide is generally a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 何雪梅孙健李丽刘国明李昌宝郑凤锦盛金凤唐雅园李杰民零东宁
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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