Method for promoting growth of probiotics by adding traditional Chinese medicine components into grain culture medium

A technology of traditional Chinese medicine components and probiotics, which is applied in microorganism-based methods, adding compounds to stimulate growth, biochemical equipment and methods, etc., can solve the problem of probiotic proliferation. There is no literature report, no report, and Cordyceps polysaccharide is rare. and other problems, to achieve the effect of increasing regulation, improving growth and viable bacteria, and promoting growth

Inactive Publication Date: 2017-05-31
吉林省浦生泰生物技术有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, research on Cordyceps polysaccharides fermented by probiotics is still rare
[0009] However, there is no literature report on the research on the proliferation effect of traditional Chinese medicine components on probiotics, and there are no reports on the microecological preparations added with traditional Chinese medicine components.

Method used

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  • Method for promoting growth of probiotics by adding traditional Chinese medicine components into grain culture medium
  • Method for promoting growth of probiotics by adding traditional Chinese medicine components into grain culture medium
  • Method for promoting growth of probiotics by adding traditional Chinese medicine components into grain culture medium

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Experimental program
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Embodiment 1

[0031] 1) Preparation of Chinese medicine component solution: Add mulberry leaf extract or Cordyceps polysaccharide (50%) to distilled water at a concentration of 100 mg / mL respectively to prepare a water-concentrated solution of Chinese medicine components, stir until dissolved, and sterilize at 115°C 30min.

[0032] 2) The configuration of the enrichment medium: add the Chinese medicine component solution prepared in step (1) to the improved MRS medium at a certain concentration, so that the final concentrations of the Chinese medicine components are 0.5 mg / mL and 1 mg / mL respectively , 5 mg / mL. The improved lactic acid bacteria culture medium (improved MRS) was prepared and sterilized at 115°C and 0.1 Mpa for 30 min, and cooled naturally after sterilization. The formula is as follows:

[0033] Element Dosage (g) Peptone 20.0 Beef Extract 10.0 Yeast extract 5.0 Diammonium hydrogen citrate [(NH 4 ) 2 HC 6 h 5 o 7 ]

4.0 Gluc...

Embodiment 2

[0038] 1) Preparation of traditional Chinese medicine component solution: add Scutellaria baicalensis, yam, kudzu root, Polygonatum odoratum, white kidney bean, grape seed, green tea, and astragalus into DMSO, stir until dissolved, and sterilize at 115°C for 30 minutes. The traditional Chinese medicine components are prepared as a DMSO concentrated solution of the traditional Chinese medicine components at a concentration of 100 mg / mL.

[0039] 2) Configuration of the enrichment medium: add the Chinese medicine component solution prepared in step (1) to the grain medium at a certain concentration, so that the final concentration of the Chinese medicine component is 0.1 mg / mL. The grain medium configuration method is as follows: 8 kinds of 80-100-mesh grain puffed powder (oat, buckwheat, sorghum, corn, barley, mung bean, black bean, soybean) are configured into a fermentation medium according to a total solid content of 8%. , in which the content of various grain components in ...

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Abstract

The invention provides a method for promoting growth of probiotics by adding traditional Chinese medicine components into a grain culture medium. The method comprises the following steps: inoculating the probiotics into an improved MRS (Methicillin-Resistant Staphylococcus) lactobacillus culture medium or a mixed grain puffed powder culture medium added with different traditional Chinese medicine components and fermenting. Each liter of the culture medium contains 5g of mulberry leaf extract or cordyceps polysaccharide or a mixture of a plurality of traditional Chinese medicines. The probiotics are lactobacillus rhamnosus or a plurality of types of mixed probiotics. According to the method provided by the invention, the fermentation speed of the probiotics in a fermentation solution and the active bacteria number of the probiotics in the fermentation solution can be remarkably improved; the mulberry leaf extract or the cordyceps polysaccharide is added; the culture medium is fermented for 12h and the active bacteria number reaches 10<9>CFU / mL; after the grain culture medium added with the traditional Chinese medicine components is fermented, the active bacteria number reaches 4.1*10<8>CFU / mL. Meanwhile, an experiment method for detecting the active bacteria number of the probiotics in a turbid fermentation system by adopting flow cytometer detection is established.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for adding traditional Chinese medicine components to a grain culture medium to promote the growth of probiotics. Background technique [0002] Probiotics are a general term for living microorganisms that are colonized in the human intestinal tract and reproductive system, can produce definite health effects and improve the host’s micro-ecological balance, and play an important role in human health. They are widely used in the production of food, health products and pharmaceuticals middle. LGG (ATCC 53103) is a patented Lactobacillus rhamnosus isolated from healthy humans by two American professors in the early 1980s. It is also one of the most researched probiotics in the world. A large number of in vitro experiments, animal experiments, human experiments and epidemiological investigations have fully proved the safety of LGG. In terms of functionality, LGG can si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12R1/225C12R1/25C12R1/245C12R1/01
CPCC12N1/38C12N1/20
Inventor 于大海房学迅杨吉雨
Owner 吉林省浦生泰生物技术有限责任公司
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