Preparation for immunosensor simultaneously using two methods for mutual verification and application thereof
An immunosensor and solution technology, applied in the field of new functional materials and biosensing detection, can solve the problems of reducing and increasing sensor sensitivity, and achieve the effects of increasing adsorption capacity, increasing stability and sensitivity, and good adsorption performance
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Embodiment 1
[0025] Example 1 A method for preparing an immunosensor that uses two methods to verify each other at the same time
[0026] (1) Polish the glassy carbon electrode with 1.0, 0.3, 0.05 µm alumina powder in turn, clean it with ultrapure water, and then place the electrode in 5 mmol / L potassium ferricyanide solution at -0.2~0.6 Scan at V potential, so that the peak potential difference is less than 110mV;
[0027] (2) 6 μL of cobalt sulfide / graphene oxide composite solution functionalized with 1 mg / mL gold nanoparticles was added dropwise to the surface of the electrode, and dried at room temperature;
[0028] (3) Continue to add 6 µL of prostate-specific antigen capture antibody solution with a concentration of 8 to 12 µg / mL dropwise onto the surface of the modified electrode, dry at 4 ºC, and rinse with ultrapure water;
[0029] (4) Block non-specific active sites with 6 μL bovine serum albumin solution with a mass fraction of 0.1%, dry at 4 ºC, and rinse with ultrapure water;...
Embodiment 2
[0032] Example 2 A method for preparing an immunosensor that uses two methods to verify each other at the same time
[0033] (1) Polish the glassy carbon electrode with 1.0, 0.3, 0.05 µm alumina powder in turn, clean it with ultrapure water, and then place the electrode in 5 mmol / L potassium ferricyanide solution at -0.2~0.6 Scan at V potential, so that the peak potential difference is less than 110mV;
[0034] (2) 6 μL of cobalt sulfide / graphene oxide composite solution functionalized with gold nanoparticles at a concentration of 1.2 mg / mL was added dropwise to the surface of the electrode, and dried at room temperature;
[0035] (3) Continue to add 6 µL of prostate-specific antigen capture antibody solution with a concentration of 10 µg / mL dropwise onto the surface of the modified electrode, dry it at 4°C, and rinse with ultrapure water;
[0036] (4) Block non-specific active sites with 6 μL bovine serum albumin solution with a mass fraction of 0.12%, dry at 4°C, and rinse ...
Embodiment 3
[0039] Example 3 Preparation of Cobalt Sulfide / Graphene Oxide Composite Functionalized by Gold Nanoparticles
[0040] (1) Preparation of gold nanoparticles
[0041] 100 mL, mass fraction 0.01 % HAuCl 4 The solution was heated to boiling, then 1.5 mL, mass fraction 1% trisodium citrate solution was added and maintained for 30 minutes, after about 1 minute the boiling solution turned wine red, cooled to room temperature for later use;
[0042] (2) Preparation of cobalt sulfide / graphene oxide composite
[0043] First, dissolve 0.4 mmol cobalt acetate and 1.6 mmol L-cysteine in 60 mL distilled water and stir for 30 min. Next, 100 mg of graphene oxide was added, stirred for 30 min, then transferred to a high-pressure reactor, and reacted for 10 h at 200 °C. After the reaction was completed, it was cooled to room temperature and washed three times with deionized water at a speed of 8000 °C. rpm, centrifuged for 5 min, dried in a vacuum oven at 50 ºC for 12 h, and the obtained b...
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