A preparation method of hippocampal polypeptide enzymatic hydrolyzate
The technology of polypeptide enzymolysis solution and hippocampus is applied in the field of preparation of hippocampal polypeptide enzymolysis solution, which can solve the problems of undisclosed preparation method of hippocampal polypeptide enzymolysis solution, waste of resources, inability to fully utilize nutrients, etc. Avoid residue and contamination, the effect of small molecular weight
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Embodiment 1
[0028] A preparation method of hippocampal polypeptide enzymatic hydrolyzate, comprising the following steps:
[0029] (1) Degreasing of hippocampal bone powder: Freeze-dry the raw material of hippocampus at -40°C for 55 hours, pulverize it with a drug grinder and pass it through a 60-mesh sieve, take the filtrate to obtain hippocampal bone powder, and then use CO 2 Degreasing by supercritical extraction technology to obtain defatted hippocampus bone powder;
[0030] (2) One-time enzymatic hydrolysis: put hippocampal defatted bone powder and water into a glass container at a ratio of 50g / L, place the container in a water bath at 55°C for 10 minutes, add trypsin with 3% weight of hippocampal defatted bone powder Finally, adjust the pH to 8.8, and adjust the pH to 8.8 every 25 minutes. After 5 hours of enzymatic hydrolysis, place the glass container in a boiling water bath to inactivate the enzyme for 10 minutes;
[0031] (3) Second enzymatic hydrolysis: continue to place the d...
Embodiment 2
[0034] With embodiment 1, its difference is:
[0035] (1) Trypsin enzymatic hydrolysis conditions: put hippocampal defatted bone powder and water into a glass container at a ratio of 40 g / L, place the container in a water bath at 40°C for 8 minutes, add 2% hippocampal defatted bone powder by weight Adjust the pH to 8.5 after trypsin, adjust the pH to 8.5 every 20 minutes, and enzymatically hydrolyze for 4 hours;
[0036] (2) Alkaline protease enzymatic hydrolysis conditions: continue to place the deactivated glass container in a water bath at 50°C for a certain period of time, add 2% alkaline protease by weight of hippocampal defatted bone powder, and then adjust the pH to 8.5. Adjust the pH to 8.5 once every 20 minutes, and enzymatically hydrolyze for 6 hours.
Embodiment 3
[0038] With embodiment 1, its difference is:
[0039] (1) Trypsin hydrolysis conditions: put hippocampal defatted bone powder and water into a glass container at a ratio of 60g / L, place the container in a water bath at 50°C for 12 minutes, add 4% hippocampal defatted bone powder Adjust the pH to 9 after trypsin, adjust the pH to 9 every 30 minutes, and enzymatically hydrolyze for 6 hours;
[0040] (2) Alkaline protease enzymatic hydrolysis conditions: continue to place the deactivated glass container in a water bath at 60°C for a certain period of time, add 4% alkaline protease by weight of hippocampus defatted bone powder, and adjust the pH to 9.5. Adjust the pH to 9 once every 30 minutes, and enzymatically hydrolyze for 7 hours.
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