A preparation method of hippocampal polypeptide enzymatic hydrolyzate

The technology of polypeptide enzymolysis solution and hippocampus is applied in the field of preparation of hippocampal polypeptide enzymolysis solution, which can solve the problems of undisclosed preparation method of hippocampal polypeptide enzymolysis solution, waste of resources, inability to fully utilize nutrients, etc. Avoid residue and contamination, the effect of small molecular weight

Inactive Publication Date: 2016-01-27
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seahorse or sea dragon medicinal wine, sea horse or sea dragon dry goods cannot make full use of the nutrients of each part of sea horse or sea dragon, which will cause some waste of resources
At present, there are no relevant research reports on the preparation method of hippocampal polypeptide hydrolyzate at home and abroad.

Method used

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  • A preparation method of hippocampal polypeptide enzymatic hydrolyzate
  • A preparation method of hippocampal polypeptide enzymatic hydrolyzate
  • A preparation method of hippocampal polypeptide enzymatic hydrolyzate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of hippocampal polypeptide enzymatic hydrolyzate, comprising the following steps:

[0029] (1) Degreasing of hippocampal bone powder: Freeze-dry the raw material of hippocampus at -40°C for 55 hours, pulverize it with a drug grinder and pass it through a 60-mesh sieve, take the filtrate to obtain hippocampal bone powder, and then use CO 2 Degreasing by supercritical extraction technology to obtain defatted hippocampus bone powder;

[0030] (2) One-time enzymatic hydrolysis: put hippocampal defatted bone powder and water into a glass container at a ratio of 50g / L, place the container in a water bath at 55°C for 10 minutes, add trypsin with 3% weight of hippocampal defatted bone powder Finally, adjust the pH to 8.8, and adjust the pH to 8.8 every 25 minutes. After 5 hours of enzymatic hydrolysis, place the glass container in a boiling water bath to inactivate the enzyme for 10 minutes;

[0031] (3) Second enzymatic hydrolysis: continue to place the d...

Embodiment 2

[0034] With embodiment 1, its difference is:

[0035] (1) Trypsin enzymatic hydrolysis conditions: put hippocampal defatted bone powder and water into a glass container at a ratio of 40 g / L, place the container in a water bath at 40°C for 8 minutes, add 2% hippocampal defatted bone powder by weight Adjust the pH to 8.5 after trypsin, adjust the pH to 8.5 every 20 minutes, and enzymatically hydrolyze for 4 hours;

[0036] (2) Alkaline protease enzymatic hydrolysis conditions: continue to place the deactivated glass container in a water bath at 50°C for a certain period of time, add 2% alkaline protease by weight of hippocampal defatted bone powder, and then adjust the pH to 8.5. Adjust the pH to 8.5 once every 20 minutes, and enzymatically hydrolyze for 6 hours.

Embodiment 3

[0038] With embodiment 1, its difference is:

[0039] (1) Trypsin hydrolysis conditions: put hippocampal defatted bone powder and water into a glass container at a ratio of 60g / L, place the container in a water bath at 50°C for 12 minutes, add 4% hippocampal defatted bone powder Adjust the pH to 9 after trypsin, adjust the pH to 9 every 30 minutes, and enzymatically hydrolyze for 6 hours;

[0040] (2) Alkaline protease enzymatic hydrolysis conditions: continue to place the deactivated glass container in a water bath at 60°C for a certain period of time, add 4% alkaline protease by weight of hippocampus defatted bone powder, and adjust the pH to 9.5. Adjust the pH to 9 once every 30 minutes, and enzymatically hydrolyze for 7 hours.

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Abstract

The invention discloses a preparation method of hippocampus polypeptide enzymatic hydrolysis solution, which is characterized in that it comprises the following steps: a. mixing hippocampus bone powder degreased by CO2 supercritical fluid extraction technology and water into a glass container according to the ratio of 40-60g / L Put it in a water bath, keep it warm at 40-50°C for 8-12 minutes, then add trypsin at a ratio of 2-4%, adjust the pH to 8.5-9, enzymatically hydrolyze for 4-6 hours, then inactivate the enzyme for 10 minutes; b. Continue to place the inactivated glass container in a water bath and keep it warm at 50-60°C for a period of time, then add 2-4% alkaline protease, adjust the pH to 8.5-9.5, and inactivate the enzyme activity after 5-7 hours of enzymatic hydrolysis Centrifuge for 10 minutes under the condition of 5000r / min, and take the supernatant to obtain the product. The advantage is that the process is simple, the operation is convenient, and the polypeptide prepared by this method has a high degree of hydrolysis, and the molecular weight of the polypeptide fragment is small, which is conducive to human digestion and absorption.

Description

technical field [0001] The invention relates to the field of marine drug development, in particular to a preparation method of hippocampus polypeptide enzymatic hydrolyzate. Background technique [0002] The use of hippocampus as a traditional Chinese medicinal material has a long history in our country. In Liang Dynasty Tao Hongjing's "Compendium of Materia Medica", it has been recorded that hippocampus is warm in nature and sweet in taste, and returns to the liver and kidney channels. It can cure respiratory disorders, sexual dysfunction, insomnia, fatigue, sore throat, skin diseases, dystocia and other diseases." It is called "water horse". In 739 A.D., Chen Zangqi used the name of hippocampus for the first time in "Supplements to Materia Medica" and it is still used today. "Compendium of Materia Medica" records that "mainly dystocia and blood gas pain, warm water and dirty, aphrodisiac, eliminate lumps, and treat sores, swelling and pain." . Each edition of the modern ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCY02P20/54
Inventor 徐永健姜展志宿宇婷
Owner NINGBO UNIV
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