Effective water chestnut virus detoxification method

Abstract

The invention belongs to the technical field of plant tissue culture, and relates to an effective water chestnut virus detoxification method, which comprises the following steps of carrying out tissue culture and rapid propagation to produce tissue culture proliferation seedlings, carrying out heat treatment on the proliferation seedlings, cutting stem tips of bud seedlings survived after heat treatment, and finally applying in agricultural production after determining the seedlings to be virus-free through detection. The effective water chestnut virus detoxification method has the advantages that a method of combining water chestnut tissue culture proliferation seedling heat treatment and stem tip cutting culture is adopted for detoxifying, the larger steam tips can be stripped and cut for culturing, and the stem tip culture survival rate is improved. The heat treatment has the effects of passivating virus activity, slowing down the speed of virus invading (upgoing) to the stem tips, and even enabling virus to lose the infection ability; in addition, the growth speed of the stem tips subjected to heat treatment is accelerated, the areas, not invaded by virus, of the stem tips are enlarged, the larger steam tips can be stripped and cut for culturing, plant buds with vigorous vitality are purified through survival of the fittest, and the success rate in detoxifying the water chestnut stem tips is improved by 10 to 15 times.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to an effective detoxification method for water chestnut virus. Background technique [0002] Water chestnut (Eleocharis tuberosa) is a perennial shallow water herb belonging to Cyperaceae. . The water chestnut skin is dark purple red, the flesh is white, the taste is sweet and juicy, crisp and refreshing, and has the reputation of "underground Sydney" since ancient times, and northerners regard it as "Jiangnan ginseng". Water chestnuts can be used as both fruit and vegetable. It is a fruit and vegetable dual-purpose economic crop. It has rich nutritional and health value, and also has the functions of clearing away heat, reducing phlegm and eliminating accumulation, diuresis, lowering blood pressure, and preventing and fighting cancer. Its extraction It has a strong inhibitory effect on bacteria, yeast and mold, and is a good health food. It can be processed into sta...

AI Technical Summary

Problems solved by technology

[0005] Water chestnuts are generally detoxified by cutting and culturing stem tips, but stem tip detoxification requires a cutting length of 0.3-0.5mm, which is difficult to cut for water chestnuts, because water chestnuts are different from other plants. It has degenerated. The clumps of green onions we see are not leaves, but leafy stems. The leaf primordium of water chestnuts is difficult to find, and the natural stem tip is also difficult to cut. Even if the cutting is successful, the cultivation process is very slow and easy to die. Success negligible