Method for measuring target component and device for measuring target component
A measurement method and a technology of a measurement device, which are applied in the direction of measurement devices, analysis materials, and material analysis through electromagnetic means, and can solve problems such as influence
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Embodiment 1
[0105] The oxidation potential was measured using the biosensor comprising the above-mentioned redox catalyst, the above-mentioned electron transport substance, and the above-mentioned electrode system, and it was confirmed that the target component could be measured by the measurement method of the present invention.
[0106] (1) Fabrication of biosensors
[0107] The aforementioned biosensors such as figure 1 Shown in the following order to make. First, if figure 1 As shown in (A), as the insulating substrate 11 of the glucose sensor, a PET (polyethylene terephthalate) substrate (50 mm in length, 6 mm in width, and 250 μm in thickness) was prepared, and one surface thereof was screen printed A carbon electrode system composed of a working electrode 12 and a counter electrode 13 each having a lead portion is formed.
[0108] Next, if figure 1 As shown in (B), an insulating layer 14 is formed on the above electrodes. First, an insulating resin polyester was dissolved in a...
Embodiment 2
[0134] According to the measurement method of the present invention, it was confirmed that the influence of the hematocrit in the measurement of the target component is reduced.
[0135] Glucose Sample 3 was prepared to contain a defined Ht value (0, 20%, 42%, or 70%) of 336 mg / dL or 600 mg / dL glucose. And, except that the above-mentioned glucose sample 3 was used instead of the above-mentioned glucose sample 1, in the same manner as the condition (1-2) of the above-mentioned Example 1 (3), the time of the above-mentioned second voltage application was used as a reference to measure The current value for 0.2 seconds. Then, the degree of influence of the Ht value when the current value of the glucose sample 3 having an Ht value of 42% was used as a reference was calculated based on the following formula (1). In addition, as a control, instead of the above-mentioned first voltage and the above-mentioned second voltage, as a condition of a comparative example, a voltage of 200 m...
Embodiment 3
[0142] When applying various first voltages and second voltages satisfying the conditions of the first voltage and the second voltage in the measurement method of the present invention, it was confirmed that the influence of the hematocrit in the measurement of the target component was reduced.
[0143] Using glucose sample 3 containing 336 mg / dL glucose, as the conditions of the example, the above-mentioned first voltage and the above-mentioned second voltage were applied under the voltage application conditions of the following (3-1) to (3-11), except that In the same manner as in Example 1 (3), the current value was measured for 0.05 seconds or 0.2 seconds based on the second voltage application. And, for each measurement time, the degree of influence of the Ht value was calculated in the same manner as in Example 2 above. In addition, regarding the control, the degree of influence of the Ht value was similarly calculated except that the voltage was applied under the condit...
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