Method for fermentation production of L-lactic acid by using surface immobilization technology

An immobilization, lactic acid technology, applied in the field of bioengineering, can solve the problems of low L-lactic acid fermentation yield, etc., achieve high economical value, increase cell density, and reduce the effect of substrate inhibition

Inactive Publication Date: 2017-06-30
NANJING UNIV OF TECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for fermenting and producing L-lactic acid using surface immobilization technology to solve the problem of low yield of L-lactic acid immobilized fermentation in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for fermentation production of L-lactic acid by using surface immobilization technology
  • Method for fermentation production of L-lactic acid by using surface immobilization technology
  • Method for fermentation production of L-lactic acid by using surface immobilization technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In this example, cornstarch is used as raw material, and the feed form of synchronous saccharification and fermentation is used for immobilization and repeated batch fermentation. The method steps are as follows:

[0040]Plate medium (g / L): glucose 5g / L, yeast extract 3g / L, peptone 10g / L, beef extract 3g / L, magnesium sulfate heptahydrate 0.5g / L, calcium carbonate 3g / L;

[0041] Fermentation medium (g / L): cornstarch 150g / L, yeast extract 15g / L, magnesium sulfate heptahydrate 0.5g / L, peptone 0.5g / L, anhydrous sodium acetate 3g / L.

[0042] (1) Plate culture: inoculate the frozen-preserved bacterial strains on the plate medium, and culture them statically at 50° C. for 48 hours in a constant temperature incubator to activate them.

[0043] (2) Seed culture: Pick bacteria from the activated plate and put them into 100ml seed culture medium, culture at 50°C and 100rpm for 24h to form a primary seed liquid. Then the primary seed solution was transferred to 500 ml of seed cult...

Embodiment 2

[0052] In this embodiment, immobilization and repeated batch fermentation are carried out in the form of single batch feeding, and the specific method steps are as follows:

[0053] Plate culture medium and seed culture medium are the same as embodiment 1.

[0054] Fermentation medium (g / L): glucose 150g / L, yeast extract 15g / L, magnesium sulfate heptahydrate 0.5g / L, peptone 0.5g / L, anhydrous sodium acetate 3g / L.

[0055] (1) plate culture: with embodiment 1;

[0056] (2) seed culture: with embodiment 1;

[0057] (3) Immobilized repeated batch fermentation:

[0058] ①Construction of the immobilization reaction system: Fill the corncobs in the stainless steel bracket 4 with mesh grooves and put them into the jacketed stainless steel column to construct the immobilization reactor, and other steps are the same as the step ① of Example 1.

[0059] ② The immobilization process is the same as the step ③ in Example 1.

[0060] ③Repeated batch fermentation process: after the immobi...

Embodiment 3

[0065] In this embodiment, cornstarch hydrolyzed sugar solution is used as raw material, and immobilization and repeated batch fermentation are carried out in the form of fed batches. The specific method steps are as follows:

[0066] Plate medium and seed culture are the same as in Example 1.

[0067] Fermentation medium (g / L): 60 g / L of corn starch hydrolyzed sugar solution, 15 g / L of yeast extract, 0.5 g / L of magnesium sulfate heptahydrate, 0.5 g / L of peptone, and 3 g / L of anhydrous sodium acetate.

[0068] (1) plate culture: with embodiment 1;

[0069] (2) seed culture: with embodiment 1;

[0070] (3) Immobilized repeated batch fermentation:

[0071] ①Construction of the immobilization reaction system: fix the activated carbon fiber on the periphery of the stainless steel support and put it into a stainless steel column with a jacket to construct the immobilization reactor. The other steps are the same as the step ① of Example 1.

[0072] ②Pretreatment of fermentation m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for fermentation production of L-lactic acid by using surface immobilization technology. The method comprises the following steps: (1) immobilization of a bacterial strain: inoculating a fermentation tank with Bacillus spp seed liquid, carrying out culturing for 48 to 108 h under the conditions of a temperature of 40 to 60 DEG C, the pH value of the fermentation broth of 5.5 to 7.0 and a stirring speed of 50 to 200 rpm, and then pumping a culture product in the fermentation tank into an immobilization reactor for circulation at a circulation flow velocity of 30 to 100 mL / min for 72 to 132 h; and (2) fermentation: pumping out culture mediums in the fermentation tank and the immobilization reactor, then pumping a novel fermentation culture medium and beginning immobilization fermentation. Compared with single-batch free cell fermentation in the prior, single-batch fermentation in the invention has the following advantages: a fermentation rate is increased by 0.62 to 0.88 g / L / h; fermentation time is shortened to 72 to 132 h from original 144 to 168 h; and product yield is increased by 1.74 to 15.42% (w / w).

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for producing L-lactic acid by repeated batch fermentation using surface immobilization technology. Background technique [0002] L-lactic acid is a precursor for the synthesis of various chiral substances, and it is widely used in food, medicine and cosmetics. The main function of L-lactic acid is that it can be used as a precursor for the synthesis of polylactic acid, which has similar gloss and processing properties to polystyrene. Products made of polylactic acid have good biocompatibility and can be used to make medical materials such as intraosseous fixation, surgical sutures, and drug-controlled release carriers; they can also replace polyethylene, polypropylene, and polystyrene, etc. Material production of new environmentally friendly packaging materials. [0003] At present, fermentation is the main method to produce lactic acid, but the pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P7/56C12N11/12C12R1/07
CPCC12N11/12C12P7/56
Inventor 应汉杰承豪陈勇邹亚男赵南柳东牛欢清吴菁岚庄伟
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap