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Method for producing D-lactic acid through repeated batch fermentation by utilizing surface immobilization technology

A secondary fermentation, lactic acid technology, applied in the direction of fermentation, can solve the problems of limited laboratory-scale operation, single immobilization carrier, affecting the fermentation rate, etc., to reduce substrate inhibition, high economic utilization value, and improve cell The effect of density

Active Publication Date: 2014-02-05
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional immobilization method mainly has the problem of mass transfer, which will cause a large amount of dead cells to accumulate in the late stage of fermentation and affect the fermentation rate
Professor Yang Shangtian of Ohio State University in the United States invented a new type of cell immobilization reactor in 1992 - the fiber bed bioreactor is used for immobilized fermentation, mainly to make the bacteria adsorb on the surface of the carrier to form high-density culture, but The immobilization carrier is single, mainly cotton fabrics, and the scope of application at this stage is small and limited to laboratory-scale operations. There is no literature report on the application of this immobilization technology to D-lactic acid.

Method used

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  • Method for producing D-lactic acid through repeated batch fermentation by utilizing surface immobilization technology
  • Method for producing D-lactic acid through repeated batch fermentation by utilizing surface immobilization technology
  • Method for producing D-lactic acid through repeated batch fermentation by utilizing surface immobilization technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] In this example, cornstarch is used as raw material, and the feed form of synchronous saccharification and fermentation is used for immobilization and repeated batch fermentation. The method steps are as follows:

[0049] Plate medium (g / L): glucose 80g / L, yeast extract 8g / L, anhydrous sodium acetate 2g / L, anhydrous magnesium sulfate 0.8g / L, KH 2 PO 4 2g / L, ferrous sulfate 0.1g / L, manganese sulfate 0.1g / L; seed medium (g / L): glucose 30g / L, peptone 10g / L, anhydrous magnesium sulfate 0.4g / L, ferrous sulfate 0.1g / L, manganese sulfate 0.1g / L, CaCO 3 20g / L;

[0050] Fermentation medium (g / L): corn starch 150g / L, yeast extract 6.5g / L, anhydrous magnesium sulfate 0.8g / L, anhydrous sodium acetate 4g / L, ferrous sulfate 0.1g / L, manganese sulfate 0.1 g / L.

[0051] 1) Plate culture: inoculate the frozen-preserved bacterial strains on the plate medium, and culture them statically at 35°C for 60 hours in an anaerobic box to activate them.

[0052] 2) Seed culture: Pick bacteria ...

Embodiment 2

[0061] In this embodiment, immobilization and repeated batch fermentation are carried out in the form of single batch feeding, and the specific method steps are as follows:

[0062] Plate culture medium and seed culture medium are the same as embodiment 1.

[0063] Fermentation medium (g / L): glucose 150g / L, yeast extract 4g / L, corn steep liquor 15ml / L, anhydrous magnesium sulfate 1g / L, anhydrous potassium acetate 4g / L, ferrous sulfate 0.01g / L, Manganese sulfate 0.01g / L.

[0064] 1) plate culture and 2) seed culture are the same as step 1) and step 2) of Example 1.

[0065] 3) Immobilized repeated batch fermentation:

[0066] ①Construction of the immobilization reaction system: Fill the corncobs in the stainless steel bracket 4 with mesh grooves and put them into the jacketed stainless steel column to construct the immobilization reactor, and other steps are the same as the step ① of Example 1.

[0067] ② The immobilization process is the same as the step ③ in Example 1.

...

Embodiment 3

[0073] In this embodiment, cornstarch hydrolyzed sugar solution is used as raw material, and immobilization and repeated batch fermentation are carried out in the form of fed batches. The specific method steps are as follows:

[0074] Plate culture medium and seed culture medium are the same as embodiment 1.

[0075] Fermentation medium (g / L): 60g / L cornstarch hydrolysis sugar solution, 20g / L yeast extract, 1g / L anhydrous magnesium sulfate, 2.5g / L anhydrous potassium acetate, 0.04g / L ferrous sulfate, sulfuric acid Manganese 0.04g / L.

[0076] 1) plate culture and 2) seed culture are the same as step 1) and step 2) of Example 1.

[0077] 3) Immobilized repeated batch fermentation:

[0078] ①Construction of the immobilization reaction system: fix the activated carbon fiber on the periphery of the stainless steel support and put it into a stainless steel column with a jacket to construct the immobilization reactor. The other steps are the same as the step ① of Example 1.

[007...

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Abstract

The invention discloses a method for producing D-lactic acid through repeated batch fermentation by utilizing a surface immobilization technology. The method comprises the steps of immobilization reaction system construction, an immobilization process and a repeated batch fermentation process. The surface immobilization technology utilizes the fiber material as an immobilization carrier, has the characteristics of high void ratio and specific area, improves the fixed mode of the fiber material, enhances the cell density, enhances the fermenting rate and can prevent the problem of mass transfer of a common immobilization technology. The repeated batch fermentation technology can save the seed culture time, thereby greatly saving the time and labor force of seed culture. According to the invention, the fermentation liquid is unnecessary to retain to be used as the seed liquid of a next batch after each batch is finished and the thalli fixed by the fiber material can be directly utilized to carry out fermentation through the combination of the surface immobilization technology and the repeated batch fermentation technology, so that the process operation is further simplified, and the stability of long-term fermentation is ensured.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for producing D-lactic acid by repeated batch fermentation using surface immobilization technology. Background technique [0002] D-lactic acid is a precursor for the synthesis of various chiral substances, and it is widely used in polymer materials, pesticides and chemicals. The main function of D-lactic acid is that it can be used as a precursor for the synthesis of polylactic acid, which has similar gloss and processing properties to polystyrene. Products made of polylactic acid have good biocompatibility and can be used to make medical materials such as intraosseous fixation, surgical sutures, and drug-controlled release carriers; they can also replace polyethylene, polypropylene, and polystyrene, etc. Material production of new environmentally friendly packaging materials. [0003] At present, fermentation is the main method to produce lac...

Claims

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Application Information

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IPC IPC(8): C12P7/56
Inventor 应汉杰陈勇赵婷柳东任恒飞施欣驰赵南陈小春吴菁岚庄伟
Owner NANJING UNIV OF TECH
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