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Method for researching influence of turbidity on development and metamorphosis attachment of stichopus japonicus larvae

A technology of larvae and metamorphosis, applied in fish farming, application, animal husbandry, etc., can solve changeable and complex problems

Inactive Publication Date: 2017-07-04
QINGDAO QINGQUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the japonicus enters the wild environment from the early planktonic larval stage, it is more complex and variable compared with indoor cultivation conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention will be described in further detail below in conjunction with specific embodiments.

[0014] The collected fresh sea mud on the surface of Rongcheng Moon Lake was filtered twice through a 200-mesh standard sieve to remove large particles, and then centrifuged in a centrifuge at 3000 r / min for 10 min to discard the water, and stored at 4°C for later use. The inactivated sea mud was boiled for 30 min before centrifugation, and other processing steps were the same as fresh sea mud. The organic matter contents of fresh sea mud and inactivated sea mud were 5.36 ± 0.33% and 3.98 ± 0.23%, respectively. The experiment was carried out in an 8L transparent plastic bucket. The experimental larvae were in the gastrula stage, the distribution density was 0.5 ind / ml (about 4000 larvae per bucket), and the temperature was controlled in a water bath of 20 ± 0.5 °C. Five concentration gradients were set in SPM, and the experiment was carried out twice, with 3 para...

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PUM

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Abstract

The invention relates to a method for researching influence of turbidity on development and metamorphosis attachment of stichopus japonicus larvae. According to the method, specifically, larvae which are in a gastrula period are selected, the arrangement density is 0.5 ind / mL, the temperature is controlled to be 20+ / -0.5 DEG C through a water bath, SPM (suspended particulate matter) with five concentration gradients are set, experiments are carried out twice, three parallel experiments are carried out each time, the concentration of each group in the first experiment is as follows: a control group, 30 mg / L, 60 mg / L, 100 mg / L and 300 mg / L, and the concentration of each group in the second experiment is as follows: a control group, 50 mg / L, 150 mg / L, 350 mg / L and 550 mg / L. The method disclosed by the invention can be used for effectively and comprehensively researching the influence of turbidity on the development and metamorphosis attachment of the stichopus japonicus larvae. Suspended particulate matters in a surface layer sea mud simulated water body from the Yue lake in Rongcheng are used, the influence of suspended particulate matters with different concentrations on growth and development of the stichopus japonicus larvae is researched, and reference can be provided for breeding work of the stichopus japonicus ecological seedlings in the open sea area.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a research method for the influence of turbidity on the development and abnormal attachment of sea cucumber larvae. Background technique [0002] Sea cucumber ecological seed breeding is a method based on ecological principles, making full use of natural environmental conditions, adopting high-efficiency facilities and ecological breeding mode, and producing high-quality and healthy seedlings through scientific management. Since sea cucumber enters the wild environment from the early stage of planktonic larvae, it is more complex and changeable compared with indoor cultivation conditions. Affected by the alternation of day and night, tide, precipitation and other environmental factors, the changes of temperature, salinity, turbidity and plankton in the water body are more severe, which will inevitably affect the development and growth of A. japonicus larvae. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/30
CPCA01K61/00
Inventor 褚方强
Owner QINGDAO QINGQUAN BIOTECH CO LTD