An analysis and identification method for distinguishing different sea cucumber varieties

Abstract

An analysis and identification method for distinguishing different sea cucumber varieties is disclosed. The method includes a step of performing spicule identification by a microscope, namely a step including (1) immobilizing collected sea cucumbers with absolute ethanol, (2) cutting body wall tissues having a size of 1 cmZ X 1mm from the middles of backs of the sea cucumbers, with the tissues being adopted as analysis samples, (3) preparing three repeated analysis samples for each sample, cleaning the samples with double-distilled water, absorbing water with sterile water absorbing paper until the samples are dry, and adding the samples to small beakers having a volume of 5 mL (with each sample being provided with an separate beaker and a separate pipet so as to prevent mutual contamination, and 4) adding dropwise 1 mL of a NaClO solution having a concentration of 100o and digesting for 30 s; and a step of sample preparing for a scanning electron microscope. Identification is performed by a method of microscope spicule differences assistant by the scanning electron microscope. Under the microscope, spicules having different shapes can be found, the spicules are an important character gradually completed and stabilized along with sea cucumber embryonic development, shapes and sizes of the spicules are greatly different for different varieties, and the spicules are an important basis for sea cucumber classification and rapid identification.

Description

technical field [0001] The invention relates to the technical field of species identification of sea cucumbers, in particular to an analysis and identification method for distinguishing different species of sea cucumbers. Background technique [0002] Sea cucumber has always been a marine treasure widely praised by people, ranking first among the eight treasures of seafood. Apostichopus japonicus is currently the only species of sea cucumber that is cultivated on a large scale in my country. However, in recent years, a series of problems such as slow growth, increased mortality, and frequent occurrence of diseases have appeared in the cultivation of sea cucumbers, and the germplasm of sea cucumbers has declined significantly. Other countries have introduced red sea cucumbers, and introduced California sea cucumbers from Canada and the United States, in order to improve the genetic diversity of sea cucumber germplasm resources, meet the current growing market demand and the ...

AI Technical Summary

Problems solved by technology

However, in recent years, a series of problems such as slow growth, increased mortality, and frequent occurrence of diseases have appeared in the cultivation of sea cucumbers, and the germplasm of sea cucumbers has declined significantly. Other countries have introduced red sea cucumbers, and introduced California sea cucumbers from Canada and the United States, in order to improve the genetic diversity of sea cucumber germplasm resources, meet the current growing market demand and the sharply declining wild resource crisis, and increase the diversity of domestic sea cucumber breeding species sex